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    Microflora Analyse in Active Sludge of Sewage Treatment and Clone and Express Nap Gene Cluster in Pseudomonas
    城市污水处理系统的菌群分析和nap基因的克隆表达
    Objective: To clone and express human prothymosin α (huPTMAα) which has important biological functions.
    目的:克隆表达具有重要生物功能的胸腺素α原cDNA(hum an prothym osinα, huPTMAα)。
    Objective: To clone and express human monocyte chemoattractant protein-1 (huMCP-1 ) with important biologic functions.
    目的:克隆表达具有重要生物功能的人单核细胞趋化蛋白1(huMCP-1)。
    Objective: To clone and express Treponema pallidum (TP) specific antigens P15 and P47 and use them in clinical examination.
    目的 :克隆表达梅毒螺旋体特异抗原 P15、P47,用于临床检测梅毒感染。
    Objective To clone and express SARS-CoV S1 protein and to construct SARS gene vaccine.
    目的 克隆表达SARS CoVS1蛋白 ,构建SARS基因疫苗。
    AIM:To clone and express SARS CoV S protein and to construct SARS gene vaccine.
    目的:克隆表达SARS-CoVS蛋白,构建SARS全长基因疫苗。
    Objective To detect HPV 58, a common type of human papillomavirus (HPV), clone and express its E7 gene from biopsy specimens of cervical cancer.
    目的 检测宫颈癌组织中人乳头瘤病毒 5 8型 (HPV5 8)并克隆表达其E7基因。
    Objective: To clone and express the MAGE-3 gene fragment (210~623nt) for researching its biological effects on MAGE-3 positive malignant tumors.
    目的克隆表达人MAGE-3基因片段(210~623位碱基),以便研究其对MAGE-3阳性恶性肿瘤的生物学作用。
    Objective To clone and express the gene encoding the binding domain of human soluble complement receptor type 1 (sCR1).
    目的克隆表达人可溶性补体受体1型(sCR1)结合C3b结构域基因。
    AIM: To clone and express the Mr 16×103 and Mr 38×103 proteins of Mycobacterium tuberculosis in E. coli, and to characterize its antigenicity and specificity.
    目的:克隆表达结核分枝杆菌Mr16×103和Mr38×103重组蛋白,测定其抗原性和特异性.
    Objective To clone and express the gene encoding 38 kD protein of Mycobacterium tuberculosis and perform serological diagnosis using the protein as an antigen.
    目的克隆表达结核分枝杆菌38kD抗原基因,并以此蛋白为抗原,进行分枝杆菌的血清学诊断。
    Objective:To clone and express the E2 gene of eastern equine encephalomyelitis virus(EEEV) in order to study the antigenicity of recombinant protein and to lay a foundation for development of genetic engineering diagnostic reagents.
    目的 :克隆表达东部马脑炎病毒 (easternequineencephalomyelitisvirus ,EEEV)E2基因 ,研究重组蛋白的抗原性 ,为研制东部马脑炎病毒基因工程诊断试剂奠定基础。
    To clone and express the cagA genes of four different Helicobacter pylori strains, so as to provide materials for investigating the function of different CagA protein and their association with diseases, the cagA genes of four Helicobacter pylori strains were amplified by PCR, and cloned into pinpoint TM Xa-1 T vector to construct recombinant plasmids, of which generate in-frame recombinant protein were verified by PCR and digesting with restrict enzymes, SDS-PAGE and western blots of CagA fusion protein induced by IPTG.
    克隆表达 4株幽门螺杆菌的cagA基因 ,以方便地获得大量CagA蛋白和重组表达质粒 ,为临床诊断CagA阳性幽门螺杆菌感染 ,以及进一步研究不同类型CagA功能及其与疾病关系提供材料。 PCR扩增幽门螺杆菌的cagA基因 ,克隆至PinPointTM Xa 1T载体 ,酶切鉴定连接方向 ,IPTG诱导正向连接克隆表达CagA融合蛋白并进行SDS PAGE和Westernblots鉴定。
    Objective: To clone and express aroB gene which encodes dehydroquinate synthase in E. coli in order to improve the dehydroquinate synthase product.
    目的 :克隆表达大肠杆菌脱氢奎尼酸合成酶基因aroB ,并测定其生物学活性。
    Objective : To clone and express aroE gene which encodes shikimate dehydrogenase in Escherichia coli in order to improve the shikimate dehydrogenase product and confirm its new specific activity of quinate dehydrogenase.
    目的:克隆表达大肠杆菌莽草酸脱氢酶基因aroE,并测定其生物学活性。
    Objective To clone and express hbhA gene of M.tuberculosis,and observe production of anti-HBHA antibody and its immunogenicity from mouse serum.
    目的应用克隆表达技术从大肠杆菌中获得重组的结核分枝杆菌黏附素HBHA蛋白,并以HBHA免疫小鼠观察抗HBHA抗体的产生并探讨其免疫原性。
    Aim To clone and express the Spike protein of SARS-CoV.Methods In this work,clone a special gene fragment of S protein of SARS-CoV into vector pET32a.
    目的克隆表达SARS冠状病毒的主要结构蛋白(S蛋白)。
    To clone and express the virulence gene mviN of Leptospira interrogans serovar Lai strain 017 in E. coli. BL21(DH3) and to investigate the cytotoxicity of the expressed product on ECV304 and A549 cells.
    目的克隆表达问号钩端螺旋体毒力基因mviN并观察表达产物对血管内皮细胞ECV304及肺上皮细胞A549的毒性作用。
    Objective To clone and express the gene fragments coding for the antigenic determinants of pp150(ppUL32), gp52(UL44),pp65(ppUL83), and ppUL80a from human cytomegalovirus.
    目的克隆表达人巨细胞病毒(human cytomegalovirus,HCMV)特异性强的抗原决定簇基因,制备特异性抗原。
    Objective To clone and express thanatin mutant(ThT) in Escherichia coli(E.coli) and purify it. Methods Th-T cDNA synthesized by PCR was inserted into the BamHⅠ-XhoⅠsite of pET32a to construct Th-T prokaryotic expression vector(pET32a-Th-T) in E.
    目的:在大肠杆菌中克隆表达thanatin突变体(Th-T)蛋白并纯化。
 

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