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转化率
    Else reaction conditions were also studies, as follows: 50 C, sugar to acid 1:5,pH7.5, initial water activity 0.75 , dosage of lipase 200mg, rotation speed 200r/min, after reaction for 32h, conversion of lauric can reach 69%.
    同时研究了其他反应条件,结果如下:温度50℃、底物摩尔比1:5(糖/酸)、初始水活度0.75、pH7.5、加酶量200mg、转速200r/min,反应32h月桂酸转化率达37.2%。
    Among 83 and 20 strain samples from polar soils and oceans, strain S1 was obtained for the highest trehalose conversion rate from maltose e of about 75.2%.
    按照确定的筛选方法,从两极地区采集的土壤菌样83株和极地海洋菌20株中最终筛选到一株产海藻糖合成酶转化率最高的一株菌S1,转化率为75.2%。
    Ethyl hexanoate synthesis mediated by Mutant 2113 lipase had 90% conversion at 15h reaction time that was 15.6% of contrast.
    诱变菌2113脂肪酶合成酶活的较大提高导致催化合成己酸乙酯能力明显增强,反应15h的转化率达到90%以上,所需时间只为出发菌的15.6%。
    The esterification conversion was about 90%.
    反应最终转化率可接近90%。
    At first, we got the optimum conditions for the preparation of fatty acid by the hydrolysis of rapeseed oil. For Lipase CL, the conversion rate reached to 71% under the condition of enzyme dosage 300HU/g substrate, mole ratio of water to oil 20∶1, reaction temperature 35℃ and reaction time 7h.
    确定了两种脂肪酶水解菜籽油制备脂肪酸的工艺条件:对Lipase CL,水/油摩尔比例20∶1,酶用量300HU/g底物,最适作用温度35℃,反应时间7h,水解转化率可达71%。
    However, for Novozym 435, the conversion rate reached to 85% under the condition of enzyme dosage 300HU/g substrate, mole ratio of water to oil 30∶1, reaction temperature 67.5℃ and reaction time 7h.
    对Novozym 435,水/油摩尔比例30∶1,酶用量300HU/g底物,最适作用温度67.5℃,反应时间7h,水解转化率为85%。
    At last, we study the magnifiying for cultivation by adopting Biostat? B 5L according to the optimum formula, and find the optimum aerat speed is 1.5wm, and the rate of circle is 500rpm and culture temperature is 28℃ ± 1.5℃, and initial pH is 6.5, so a high conversion rate of 60% was obtained from the light colour medium.
    最后,通过采用Biostat@B 5L发酵罐进行放大培养,发酵培养基为摇瓶所确定的最适配比,通过研究确定通气率为1. 5vvm, 搅拌速度为500rpm,培养温度为28℃±1. 5℃,初始pH值为6. 5,使得多糖转化率超过60%,发酵液颜色为浅乳黄色。
    On the conditions above,L-lactic acid productivity and the crude corn starch conversion rate were 86.80g/L and 65.17% ,respectively. The biomass concentration was 8.8g/L.
    在该最适发酵条件下,该米根霉菌株的摇瓶发酵L-乳酸积累量可达86.80g/L,对原淀粉的转化率可达65.17%,最高生物量可达8.8g/L。
    In this condition the conversion of lecithin to lysophospholipid was 86.3% determined by acid base titration and HPLC.
    在最佳反应条件下,得到的产物经酸碱滴定法及HPLC检测,大豆磷脂的转化率为86.30%。
    The yield of L-serine maintained at 7.36g/L with a26.27% molar conversion of glycine.
    菌株S63-31在优化条件下的L-丝氨酸产量(7.36g/L)比初始条件下(6.5g/L)提高了13.23% ,L-丝氨酸对甘氨酸的摩尔转化率为26.27%。
    The enzymatic conversion at the pilot scale of 2L was also studied. The substrate amountof 15g/L, the stirring rotation and aeration were adjusted to 1.0vvm and 200r/min repectively. The best production of vanillin from isoeugenol in 2L bioreactor was 2.17g/L for 60 h,corresponding to a molar conversion of 15.61%.
    进行了酶反应-分离耦合工艺的放大研究,用2L玻璃搅拌反应器, 15g/L底物浓度,1.0vvm的通气量,200r/min的磁力搅拌转速条件下,60小时香草醛量可达2.17g/L,摩尔转化率为15.61%。
    To get higher yield of L-Gln, feed-batch ofglucose and glutamate was determined. and the final yield of L-Gln is 33.8g/L and the conversion rateis67.6%.
    为了进一步提高产量,确定了葡萄糖、谷氨酸的分批三次补料转化工艺,得到谷氨酰胺较好产量为33.8g/L,转化率为67.6%。
    When the molar ratio of amine and epoxychloropropane was 1:2.5, after 1 hour reaction at 80 ℃, the conversion ratio of amine exceeded 99%.
    以环氧氯丙烷自身作溶剂,叔胺与环氧氯丙烷的摩尔比为1:2.5进行反应,80℃条件下加热1h左右,叔胺转化率可达99.6%。
    The conversion rate of L-Gln as to theanine being 48.22%.
    L-Gln转化率为48.22%。
    Compared with Suzuki' report, 20.90g/L theanine was synthesized by recombinant microbe, the conversion rate of L-Gln as to theanine being 60%.
    Suzuki等报道的用构建的重组菌催化谷氨酰胺(Gln)和乙胺合成茶氨酸的量为20.90g/L,Gln转化率为6096。
    The yield of lactic acid could exceed 90%, the current efficiency was about 60% and the electric energy consumption was between 2.5-3.5kWh/kg at 95% conversion factor of sodium lactate.
    在95%乳酸钠转化率条件下,电渗析的能耗在2.5-3.5kWh/kg左右,电流效率在60%左右,乳酸的回收率可以达到90%。
    On above conditions, L-lactic acid productivity and crude corn starch conversion rate were 88.90/L and 66.74% in 7L fermentor, respectively. The biomass concentration was 7.2g/L.
    在此条件下,该菌株的罐发酵L-乳酸浓度可达88.90g/L,对原淀粉的转化率达66.74%,最高生物量达7.2g/L,发酵周期60h。
    The system could be operated for 40 d without significant decline and the average productivity was 1.85g/L/h and the conversion yield was above 85% throughout the 40 days.
    在此条件下,填充床反应器连续运转40天,苯并酮酸的转化率均在85%以上,L-苯丙氨酸平均体积产率为1.85g/L/h,显示了固定化细胞填充床反应器较好的操作稳定性。
    The process conditions in shake flask were obtained as follows:inoculum volume 10%(seed age 36~38h), cultivate temperature 28℃, rotation speed of shake flask 220r/min, hypha age 22~24h,(pH down to about 3.6), concentration of substrate 20g/L(dissolved by 0.03% tween), conversion temperature 30℃, conversion period 72h. The conversion rate of substrate reached 70%.
    考察了一步转化工艺的培养基组成、投料时间、溶料方式等因素,确定了摇瓶反应工艺:28℃培养36~38h的种子以10%的接种量接种二级,培养22-24h(pH降至3.6左右)投入20g/L浓度的底物(0.03%吐温分散),转化温度30℃,摇床上转化72h转化率可达70%.
    The dissolved oxygen was bettered under 10L, 20L stirred-tank bioreactor and the conversion rate is closed to 60% after 45h.
    该工艺在10L、20L罐上溶氧得到改善,在底物浓度20g/L条件下转化45h转化率接近60%,可以进一步放大和推广。
 

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