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    Intracellular cyclic GMP(cGMP) levels were measured by radioimmunoassay, and the effects of a sapogenin from Zhimu (ZMS) on cGMP formation stimulated by carbachol were investigated.
    目的:观察知母皂甙元(ZMS)对原代培养神经细胞M受体刺激后cGMP生成速率的影响。 方法:用放射免疫分析法测定细胞内cGMP水平,观察ZMS不同作用时间对Carbachol刺激下cGMP生成的影响。
    The effects of l3nbutylphthalide(lNBP) and d3nbutylphthalide(dNBP) on extracellular nitric oxide(NO) levels and intracellular cyclic GMP(cGMP) levels were studied in primary cultured rat cortical neuronal cells. Nitric oxide and cGMP levels were measured by using spectrometry and radioimmunological analysis(RIA), respectively.
    用分光光度法和放射免疫分析法分别检测NO及cGMP水平,同时观察l丁基苯酞(lNBP)和d丁基苯酞(dNBP)对原代培养的胎大鼠皮层神经细胞外液NO及胞浆内cGMP水平的影响。
    Circadian rhythms of cyclic AMP,cyclic GMP and cAMP/cGMP were measured in mouse blood T lymphocytes. Upon 5Gy of γ-irradiation,the level of cAMP increased and that of cGMP decreased,leading to an augmentation of the cAMP/cGMP,while the low dose of 0\^5Gy caused a decline in the ratio.
    测定了小鼠外周血T 淋巴细胞中cAMP和cGMP含量及其比值的昼夜节律, 各峰值时相分别位于12∶20 时、01∶12 时和13∶04 时, 经5-0 Gy γ- 射线照射后, cAMP含量升高,cGMP含量降低,cAMP/cGMP比值增大。 而0-5Gy 照射引起比值下降。
    This work is designed to investigate the change of serum ANF, cyclic AMP, cyclic GMP and left ventricular function in human heart failure.
    为研究心力衰竭患者血浆cAMP、cGMP、心钠素(ANF)的变化,同时与超声左心功能指标相比较以探讨它们的互相关系。
    RIA was used to analyse serum ANF, cyclic AMP, cyclic GMP of 65 congestive heart failure patients from 1994 to 1996, whereas control group contained 15 volunteers without any cardiac disease.
    收集了65例心功能不全患者,15例正常健康对照病例,静脉血用放射免疫法测定血浆中cAMP、cGMP、ANF。
    Serum ANF, cyclic AMP, cyclic GMP were highly related to cardiac function.
    结果:血浆cAMP、cGMP、ANF的升高程度与心衰严重程度密切相关。
    Serum concentration of ANF, cyclic AMP, cyclic GMP in patients with cardiac function NYHAⅠ ̄Ⅱ were statistically higher than those in patients with normal cardiac function, while in patients with cardiac function Ⅲ ̄Ⅳ it is the highest.
    NYHAⅢ~Ⅳ级心功能患者的血浆生化指标明显高于NYHAⅠ~Ⅱ级患者,而后者则明显高于无心功能不全的健康人。 血浆cAMP、ANF与EF、ΔD呈弱负相关而与LEDV成强正相关;
    Serum concentration of ANF, cyclic AMP, cyclic GMP had strong negative correlation with ΔD and EF, but strong positive correlation with LEDV. There is a good relation between ANF and cyclic AMP, but not ANF to cyclic GMP.
    血浆cGMP与EF成强负相关,与ΔD呈很弱负相关,与LEDV成较弱正相关,血浆ANF与血浆cAMP有很好的相关性,而与cGMP有较弱的相关。
    CONCLUSION:Nitric oxide might be involved in cardiac dysfunction by modulating cyclic GMP formation and Na +-K +-ATPase activity in myocardium.
    伴之心肌cGMP含量的升高及心肌细胞膜Na+-K+-ATP酶活性的下降。 结论:一氧化氮通过调控心肌cGMP的合成及肌膜Na+-K+-ATP酶活性而影响心肌功能。
    METHOD Rabbit corpus cavernosum(RCC) segments were incubated with various concentrations of sildenafil. The formation of cGMP was stimulated with 1 μmol·L -1 sodium nitroprusside(SNP), and the cyclic GMP concentration was measured by radioimmunoassy technology.
    方法  37℃温孵兔海绵体组织(RCC) ,用放射免疫方法监测在硝普钠存在下 ,西地非尔10、10 0、10 0 0nmol·L-1对cGMP水平的影响 ;
    (3) It inhibits TXB2 release induced by AA and increases the platelet cyclic AMP level, while cyclic GMP level was not changed.
    (3)穿心莲黄酮可明显抑制AA诱导的血小板TXB_2的释放,并可明显升高血小板内cAMP的含量,对cGMP含量无明显影响。
    The effects of nitric oxide on modulation of pain in central nervous system of rats may be partly mediated by NO cyclic GMP pathway.
    (2 )中枢神经系统 NO对大鼠痛觉的调制作用至少一部分是通过 NO- c GMP途径实现的
    The effects of nitric oxide on modulation of pain in central nervous system of rats may be partially mediated by NO cyclic GMP pathway.
    中枢神经系统 NO对大鼠痛觉的调制作用至少部分是通过 NO- c GMP途径实现的。
    AIM: To study the effects of 2-[3-estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) in human os-teoblast-like cell line TE85. METHODS: [3H]Thymi-dine incorporation and cell count for cell proliferation, ra-dioimmunoassay for cyclic GMP (cGMP) content, and monitoring the conversion of [3H]arginine for inducible nitric-oxide synthase (iNOS) activity assay.
    目的:研究XW630对人的类成骨细胞株TE85细胞功能的影响。 方法:[~3H]胸腺嘧啶掺入法和细胞计数法测定细胞增殖、放射免疫法测定cGMP含量、精氨酸转化法测定iNOS活性。
    SIN-1- and SNP-in-duced increase of IK(Ca) was blocked by methylene blue 1 μmol· L -1. CONCLUSION: NO increases calcium-activated potassium currents in gastric antral circular my-ocytes of guinea pig, and the effect of NO on IK(Ca) be mediated by cyclic GMP.
    1μmol·L~(-1)亚甲蓝完全阻断硝普盐与SIN-1导致的增加效应。 结论:一氧化氮增加豚鼠胃窦环行肌钙敏感钾电流,该效应可能通过环磷酸鸟苷介导。
    Lot and zaprinast(Zap)enhanced the cyclic GMP level in rabbit corpus cavernosum in concentration dependent manner. This effect of Lot was much weaker than that of Zap.
    在家兔阴茎海绵体 ,Lot和扎普司特 (Zap)可剂量依赖性地增加海绵体cGMP含量 ,Lot的作用远弱于Zap。
    Objective To analyze the function of Nitric Oxide cyclic GMP (NO cGMP) pathway,which may relate to the effects mediated by nerve growth factor (NGF) to cerebellar granule cell (CGC) cultures.
    目的探讨神经生长因子 (NGF)对鼠小脑颗粒细胞 (CGC)存活的作用机制。
    Objective:To explore the effects of heme heme oxygenase(HO) carbon monoxide(CO) cyclic GMP(cGMP)and L Arginine nitric oxide synthase(NOS) nitric oxide(NO) cGMP on aortic vascular hyporeactivity in endotoxin shock (ES)rats.
    目的 :探讨血红素 HO CO cGMP和L 精氨酸 NOS NO cGMP通路对内毒素休克 (ES)大鼠主动脉血管张力的影响。
    Cyclic GMP (cGMP) level in myocardium was measured by radio-immunoassay.
    利用放免方法检测心肌中cGMP的水平.
    Then the rats were sacrificed and the serum obtained. The content of triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL C) and high density lipoprotein cholesterol (HDL C) were determined individually. Cyclic AMP、cyclic GMP and nitric oxide were detected in the liver homogenate and the light microscope and electron microscope preparations were made for certain liver tussue.
    实验结束后采血分离血清测甘油三酯 (TG)、总胆固醇 (TC)、低密度脂蛋白胆固醇 (LDL C)、高密度脂蛋白胆固醇 (HDL C) ,取部分肝脏左叶测一氧化氮 (NO)、环磷酸腺苷 (cAMP)、环磷酸鸟苷 (cGMP)并做光镜、电镜标本。
 

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