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    Callus Formation Enhanced by BMP-7 Gene Modified Autologous Bone Marrow MSCs in Rat Mandibular Distraction Osteogenesis
    BMP-7基因修饰的自体骨髓间充质干细胞促进大鼠下颌牵张成骨的实验研究
    The Effect of Bone Growth Factors on Distraction Osteogenesis
    骨生长因子对牵张成骨的作用
    Conclusion:0.8 mm/day may be the best distraction rate in mandibular distraction osteogenesis.
    结论:采用0.8mm/d的牵张速率进行牵张能最快促进新骨形成,提高成骨质量。
    Objective To analyze the elastic efficiency of two kinds of titanium nickle shape memory alloy(Ni-Ti-SMA) (C3020φ1. 0mm and B2025φ0.8mm), and choose the optimal one to use for distraction osteogenesis of jaw bones.
    目的分析C3020φ1.0和B2025φ0.8 NiTi合金丝的性能,选择更适合牵张成骨实验的镍钛合金材料,探索镍钛合金丝在高温定型过程中最合适的记忆温度。
    Conclusion The C3020φ1.0mm titanium nickle SMA is suitabele for distraction osteogenesis experiment,In treatment temperture(450℃,500℃,550℃,600℃) ,500℃was the optimal typing temperature.
    结论规格为C3020φ1.0mm的NiTi-SMA的性能更适合用于颌骨牵张成骨实验。 在450℃、500℃、550℃、600℃4个不同的记忆温度下,500℃定型温度效果最理想。
    Objective: To study the expression of vascular endothelial growth factor(VEGF) and its receptors during mandible distraction osteogenesis(MDO) through establishing a distraction osteogenesis experimental model in rabbit.
    目的:通过建立兔下颌骨牵引成骨实验动物模型,研究下颌骨牵引成骨过程中血管内皮生长因子(vascular endothelial growth factor,VEGF)及其受体的表达及意义。
    Six rabbits were chosen as control group. Samples were sacrificed at 7th days of latency, 2nd, 4th, 7th day of distraction, and at 1st, 3rd, 6th, 8th week of consolidation. Expression of VEGF and its receptors, fms-like tyrosine kinase (FLT-1) and fetal liver kinase(FLK-1), were detected by immunohistochemistry.
    在延迟期第7d,牵引期第2、4、7d,固定期第1、3、6、8周分别处死3只动物,采用免疫组化方法,检测VEGF及其受体FLT-1(fms-like tyrosine kinase-1)、FLK-1(fetal liver kinese-1)的表达变化。
    The specimens were retrieved at 2,4,6,8 and 12 weeks after completion of distraction.
    术后第2、4、6、8及12周分别处死3只动物,分析ALP在新骨组织中表达与分布。
    Three animals from each experiment group were sacrificed at 2,4,8 weeks at the end of distraction.
    牵张结束后2、4、8周每组各处死3只动物取材。
    At the end of 2 week's and 4 week's distraction,there were significant differences between the experiment group of injecting PRP at lengthening period and the other experiment groups through DEXA examination and histomorphology(P<0.05).
    牵张结束后2、4周时,牵张期注射PRP组骨密度测量和组织学观察与其余两实验组间差异有统计学意义(P<0.05)。
    Objective To study the expression of transformating growth factor-β1(TGF-β1) during mandible distraction osteogenesis(MDO) in a novel experimental model of rabbits.
    目的通过建立兔下颌骨牵引成骨实验动物模型,研究下颌骨牵引成骨过程中TGF-β1表达及意义。
    TGF-β1 and TGF-β1 mRNA began to express at early distraction period,peaking at D2,then decreasing slowly.
    TGF-β1mRNA及其蛋白表达在牵引早期达到高峰,此后逐渐减弱。
    Objective To study the effects of internal maxillary distraction osteogenesis(DO)on the velopharyngeal configuration of cleft palate patients.
    目的探讨内置式上颌骨牵引成骨术(distraction osteogenesis,DO)对腭裂患者腭咽部结构的影响。
    Ang-1 was slightly expressed at the latency phase,and highly expressed at the distraction period and end of1-2W consolidation phase,with the maturation of the new formed bone,at the end of 3-7W consolidation phase the expression of Ang-1 was weaken.
    Ang-1在延迟期末有轻微表达,在牵张期和牵张结束后1-2周牵张区中Ang-1的表达水平较高,随着新生骨组织的不断成熟,Ang-1的表达开始下降,在牵张结束后第3-7W,Ang-1染色较弱。
    Results A distraction rate of 2.0mm/d resulted in nonunion of the distraction gap. Aftermandibular lengthening to 15mm at 1.5mm/d,the regenerated bone was well formed,but the IAN showed irreversible injury.
    结果以2.0mm/d 速度牵引下颌骨,会产生骨不连接,以1.5mm/d 速度牵引颌骨15mm,虽可获得良好的成骨,但可造成下牙槽神经不可逆性的损伤。
    Conclusion Thesuitable rates of mandibular distraction osteogenesis should be limited between 0.5mm/d and 1.5mm/d.
    结论下颌骨牵引成骨术的速度宜控制在0.5-1.5mm/d 范围内;
    To simulate theorientation of distractors either parallel to the body of the mandible or parallel to the axis of distraction,the mandible was lengthened 5mm、10mm、15mm、20mm,analyze displacement and stress distribution on the distractors-bone interface.
    模拟牵引器放置平行于下颌骨体部及平行于牵引轴方向两种状况,分别分析下颌骨牵引延长5mm、10mm、15mm、20mm 四种工况下骨-牵引器界面位移、综合应力及应力分布情况。
    Starting with distraction,VEGF was appied in a dosage of 100 ng/d for7 day(twice daily),whereas group B was used as control.
    从牵张开始第1天在A 组动物牵张区每日注射VEGF100ng,每日2次,连续7d。 注射生理盐水的B 组动物用作对照。
    ②At the end of distraction,mierovessel density was decreased from microcolumn formation(MCF)zone to fibrous interzone(FIZ);
    ②牵张结束时,血管密度由骨小梁形成区带(MCF)向纤维区带(FIZ)递减;
    Intraoral distraction osteogenesis
    口内入路的颌骨牵引成骨技术
 

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