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基因序列
    Phylogenetic Analysis of Bluetongue Viruses in China and S10 Gene Expression of the Virus
    中国蓝舌病毒S10、L2基因序列分析及S10基因克隆表达
    Comparative Analysis and Molecular Phylogeny of Chinese Deer Based on Mitochondrial 12S rRNA Gene Sequences
    中国鹿类动物线粒体12S rRNA基因序列的比较分析及分子系统发育研究
    Study on Sequences, Molecular Evolution and Polymorphism of Horse Growth Hormone Gene
    马生长激素(GH)基因序列、分子进化及其多态性的研究
    Molecular Phylogeny of Pneumocystis Based on mt LSU rRNA and ITS1-5.8SrRNA-ITS2 Gene Sequence
    基于mtLSU rRNA和ITS1-5.8SrRNA-ITS2基因序列的肺孢子虫分子系统发育学研究
    Study on Detection Methods of Gene Mutation and Their Clinical Applications
    基因序列变异分析的方法研究及其临床应用
    Gene Sequencing of Recombinant Human IFN-α_1 after High Density Fermentation
    高密度发酵后的重组人α_1型干扰素基因序列分析
    Direct detection of HIV gene sequence by polymerase chain reaction
    应用聚合酶链反应直接检测HIV基因序列
    DNA Sequence of Xylose Isomerase Gene from Streptomyces diastaticus No. 7 Strain M1033
    7号淀粉酶链霉菌M1033木糖异构酶基因序列分析
    Sequence analysis of TuMV coat protein gene Hangzhou isolate
    芜菁花叶病毒杭州分离株外壳蛋白基因序列分析
    Sequence Analysis of TK Gene in 2.2kb Fragment of FowIpox Virus 282E4 Genome
    鸡痘病毒282E_4株2.2kbTK基因序列分析
    Analysis of Nucleotide Sequence and Pbylogeny on HA_1 Gene of Influenza B Viruses Isolated in Hebei
    乙型流感病毒河北株的HA_1基因序列及种系发生的分析
    PARTIAL 16S rRNA GENE SEQUENCING OF RHIZOBIAL STRAINS CA8561 AND JL84 ISOLATED FROM ASTRAGALUS
    黄芪根瘤菌CA8561和JL84的部分16SrRNA基因序列的测定
    SEQUENCE ANALYSIS OF THE ENDOPEPTIDASE GENE OF AN AVIRULENT EGG DROP SYNDROME VIRUS(EDSV)
    鸡减蛋综合征病毒(Egg Drop Syndrome Virus,EDSV)巯基内肽酶的基因序列分析
    A pair of primer was designed and synthesized according to nucleoprotein(NP) gene sequences of H5N1 subtype of avian influenza virus.
    根据已知H5N1亚型禽流感病毒NP基因序列设计、合成PCR克隆引物。
    According to the 16S rRNA gene sequence published in GenBank, which include Mycoplasmas gallisepticum of chicken and M.hyopneumoniae,M. hyosynoviae and M.flocculare of swine(submitted No.:Mg AY744942, Mhp AE017244, Mhs AY973563 and Mf X63377), and the PCR primers labled by Dig and the probe labled by biotin were designed by the software DNAstar and Primer 5.0. The system and condition of PCR-ELISA were optimized.
    实验依据GenBank中登录的鸡源支原体和猪源支原体16SrRNA基因序列(登录号:Mg,AY744942;Mhp,AE017244;Mhs,AY973563;Mf,X63377),运用DNAStar软件和Primer5.0软件设计PCR特异性引物和探针,其中引物用地高辛标记,探针用生物素标记。
    According to the GenBank published sequence of equine west nile virus(WNV)E protein gene,a pair of primer was designed in order to amplify equine WNV partly E gene by RT-PCR. The fragment was 318bp in length and was cloned into pMD18-T-Vector. The positive clone was named pMD-E and was sequenced.
    参考GenBank发表的西尼罗病毒(west nile virus,WNV)的E蛋白基因序列,自行设计合成一对引物,利用RT-PCR扩增出了WMV E基因318bp片段,将其克隆入pMD18-T-Vector载体中,阳性克隆命名pMD-E,并进行序列分析。
    Sequence comparison for MMOX with counterpart of other five strains showed that from 78% to 99% identity in protein level and from 71% to 97% identity in gene level, in the separate comparison of six components, only orfY component had a lower identical.
    与已发表的甲烷氧化细菌中甲烷单加氧酶进行了比较,结果表明MMOX组份中氨基酸序列的同一性为78%到99%,基因序列的同一性为71%到97%,6个组份中orfY片段的同一性相对较低。
    According to pmi gene sequence in genebank,the 6-phosphomarmose isomerase gene of Escherichia coli was amplified by PCR.
    根据genebank中公布的pmi基因序列设计引物,通过PCR扩增从大肠杆菌中分离出6-磷酸甘露糖异构酶基因。
    The content of A/T was comparatively higher in sequences of the cyt b gene of yellow cattle,yak and buffalo,compromising 56.6%,58.2% and 56.0% of the total nucleotides on average respectively.
    结果表明,中国黄牛、牦牛和水牛cyt b基因序列中碱基A和T的含量均比较高,A+T的平均含量分别为56.6%,58.2%和56.0%。
    Gene orders of p53 gene(exon 1-11)from all of 10 samples were tested.
    检测10份样本中p53基因外显子1~11的基因序列
 

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