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    Study on the Inhibition of Hepatitis B Virus (HBV) by Hepatitis Delta Virus (HDV) Ribozyme in Cells
    丁型肝炎病毒(HDV)核酶对乙型肝炎病毒(HBV)抑制作用的研究
    Study on Hepatitis Delta in Some Areas of Fujian Province
    福建省部分地区丁型肝炎的研究
    EXPERIMENTAL STUDIES OF EASTERN WOODCHUCK INFECTED WITH HEPATITIS DELTA VIRUS
    丁型肝炎病毒感染东方土拔鼠的实验研究
    HIGH- LEVEL EXPRESSION AND SECRETION OF CHINESE HENAN HEPATITIS DELTA ANTIGEN IN PROKARYOTIC CELLS
    我国河南株丁型肝炎病毒抗原在原核细胞中的高效表达及分泌
    In Vitro Transcription of cDNA of Hepatitis Delta Virus Genomic RNA Ribozyme
    丁型肝炎病毒基因组RNA中核酶区的cDNA的体外转录
    ESTABLISHMENT AND APPLICATION OF DETECTIVE METHOD OF IgM ANTIBODY TO HEPATITIS DELTA VIRUS
    丁型肝炎IgM抗体检测方法的建立和应用
    THE PERINATAL VERTICAL TRANSMISSION OF HEPATITIS DELTA VIRUS INFECTION BETWEEN GRAVIDA AND FETUS
    孕妇丁型肝炎病毒感染及其围产期母婴垂直传播的研究
    DETECTION OF SERUM HDV RNA BY POLYMERASE CHAIN REACTION IN PATIENTS WITH HEPATITIS DELTA
    聚合酶链反应检测慢性丁型肝炎血清HDV RNA的实验研究和临床观察
    Methods Hepatitis delta virus' sequence was obtained from Inner Mongolian patient by using RT-PCR and PCR methods, PET43a was used and His-tag was added at the HDV L-Ag 5′ and 3′ to construct the recombinant expression plasmid, transform the plasmid into host bacterium BL21 and induce it with IPTG.
    方法从内蒙古某丁肝患者血清中提取丁肝病毒,经RT-PCR与PCR,使用PET43a表达质粒及HDVL-Ag5′和3′端引入His标签获得丁型肝炎病毒L-Ag重组表达质粒,转化BL21(Rosetta)宿主菌,IPTG诱导表达。
    The Molecular Biology of Hepatitis delta virus
    丁型肝炎病毒的分子生物学研究进展
    The Structural Features and Catalytic Acting Mechanism of Hepatitis Delta Virus Ribozymes
    丁型肝炎病毒核酶的结构特点与催化作用机制
    Establishment and application of recombinant hepatitis delta antigen
    丁型肝炎病毒重组抗原的制备及应用
    Ribozyme Delivered by Hepatitis Delta Virus Inhibits Hepatitis B Virus Replication in Mice
    丁型肝炎病毒载体携带核酶在小鼠体内抑制乙型肝炎病毒复制
    Investigation on activities of hammerhead ribozyme embedded in genomic RNA of hepatitis delta virus
    丁型肝炎病毒基因组RNA包埋锤头状核酶的活性研究
    Expression of the hepatitis Delta antigen in prokaryotic cell and evaluation of its application as an EIA diagnostic reagent
    丁型肝炎病毒抗原的原核表达及抗原性分析
    Expression of hepatitis delta antigen Inner Mongolian strain in prokaryotic cell and analysis of its antigenicity
    丁型肝炎病毒内蒙古株的原核表达及抗原性分析
    Hepatitis delta antigen (HDAg) from the liver of the woodchuck hepatitis virus (WHV) carrier (woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column.
    用丁型肝炎病毒(HDV)感染慢性携带土拨鼠肝炎病毒的东方土拨鼠(Woodchuck)后,取其肝组织制成匀浆,离心后经Sepharose 4B柱,免疫亲和层析柱,获得高丰度的HDAg。
    Chinese hepatitis delta antigen-coding cDNA was characterized by cloning and sequencing four fragments with size of 707bp, 414bp, 376bp and 274bp respectively, from a HBsAg carrier positive for HDAg and HDV RNA from Western China Sichuan Province, using reverse trans-cription and polymerase chain reaction.
    从我国四川一丁型肝炎病毒抗原(HDAg)、HDV RNA双阳性的HBsAg携带者血清中提取RNA,采用人工合成的引物进行4次逆转录和聚合酶链反应(PCR),获得了长度分别为707bp、414bp、876bp和274bp的4个HDV cDNA片段,并对其进行了克隆和序列分析。
    wo expression plasmids pETDAg and pETDAg -1 ,which contain the gene of Chinese Henan hepatitis delta antigen were constructed under the T7 Promoter,with and without terminator resepectively .
    将我国河南株丁型肝炎(丁肝)病毒抗原的编码基因,克隆到分泌性原核表达载体pET-12a的T7启动子下游,构建了保留和去除终止子(Terminator)的两个表达载体pETDAg和pETDAg-1。
    The serum of 151 cases of gravidas with positive Hepatitis B Virus Marker (HBV -M )and unchangable liver function was detected for Hepatitis Delta Virus Marker (HDV-M ) byEnzyme Immune Assay(ElA). Theresults demonstrated that 24 cases showed HDV-M (+)(24/151, 15. 89% ).
    本文采用酶免疫测定法(EIA),检测长春地区151例乙肝病毒标志物(HBV-M)阳性,肝功能无改变的孕妇血清中丁型肝炎病毒标志物(HDV-M),结果HDV-M(+)共24例,阳性率15.89%(24/151)。
 

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