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异位成骨
    Ability of heterotopic osteogenesis of composites of bone marrow stromal cells transfected with hBMP-2 gene and bioactive glass ceramics
    转染hBMP-2基因的骨髓基质细胞与生物活性陶瓷复合材料异位成骨能力观察
    The MSCs and BGC transfected with hBMP 2 gene were of good biocompatibility and ability of heterotopic osteogenesis.
    携带hBMP-2基因的MSC与BGC具有良好的生物相容性,且具有异位成骨能力。
    CONCLUSION:MSCs can be used as the recipient cells of hBMP 2 gene for expressing and secreting the hBMP 2 protein, and the composites of the tansfected cells and BGC were proved to have the ability of heterotopic osteogenesis, indicating that transfected MSCs by hBMP 2 protein can be served as seed cells for bone tissue engineering.
    结论:MSC可以作为hBMP-2基因的受体细胞,基因表达可分泌hBMP-2蛋白质,与BGC复合后具有异位成骨能力,认为转染了hBMP-2基因的MSC可作为骨组织工程学中的种子细胞。
    AIM:To investigate the ability of heterotopic osteogenesis of the composites of rabbits' bone marrow stromal cells(MSCs) transfected with hBMP 2 gene and bioactive glass ceramics(BGC) after transplanted into the muscle strand of rabbit's thigh.
    目的:观察转染了hBMP-2基因的兔骨髓基质细胞(bonemarrowstromalcells,MSC)与生物活性陶瓷(bioactiveglassceramicsics,BGC)复合后植入兔股部肌袋中生物复合材料的异位成骨能力。 方法:实验时间为2003-07/2004-05。
    The chimeric protein had the double bio-activities of BMP 2 and EGFP identified by the cellular activity and animal heterotopic osteogenesis tests.
    分泌的产物经细胞活性实验和动物体内异位成 骨实验证实具有BMP2和EGFP的双重蛋白活性。
    Results (1) Heterotopic osteogenesis was found after intramuscular injection in nude mice, most of which were endochondral ossification.
    结果1.裸鼠肌内注射后有异位成骨,其主要为软骨化骨。
 

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