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杀虫蛋白基因
    Studies on Cloning and Expression of Vegetative Insecticidal Protein from Bacillus Thuringiensis
    苏云金杆菌营养期杀虫蛋白基因的克隆与表达研究
    The Acquirement of Transgenic Bollworm Resistant Xinjiang Cotton Plants with the Synthetic Gene Coding Bacillus Thuringiensis Insecticidal Protein
    Bt杀虫蛋白基因导入新疆棉花获得抗虫转基因植株
    EXPRESSION AND SYNERGISM OF TWO CRY INSECTICIDAL PROTEIN GENES IN PSEUDOMONAS FLUORESCENS'
    双价杀虫蛋白基因在荧光假单胞菌中的表达及增效
    DYNAMICS OF EXPRESSION OF Bt INSECTICIDAL PROTEIN GENE IN THE TRANSGENIC RAPESEED AND ITS INSECT-RESISTANCE ACTIVITY
    Bt杀虫蛋白基因在转基因油菜中的动态表达与其抗虫性研究
    Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity
    转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究
    Characteristic Analysis of Vegetative Insecticidal Protein Gene from Bacillus thuringiensis
    苏云金芽胞杆菌营养期杀虫蛋白基因特性分析
    The results show that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.
    连续三代的研究结果表明 :Bt杀虫蛋白基因以单拷贝、杂合地整合到转基因植株 (T0 1,T0 2 ,T0 3,T0 5 ,T0 6 ,T0 7,T0 8)的基因组中 ,并稳定地遗传给后代 .
    By analysing F 2 of hybrid combination of homozygous transgenic plants, Bt insecticidal protein gene has been integrated into different locus of homologous chromosome of T 01 and T 05 or T 03 and T 08 transgenic rapeseed plants,but has been integrated into heterologous chromosome.
    纯合转基因株系杂交分析表明 ,在不同 T0 转基因植株中 Bt杀虫蛋白基因整合位点是不同的 ,T0 1和 T0 5或 T0 3和 T0 8的 Bt杀虫蛋白基因整合位点是同源染色体的不同座位 ,而其他转基因植株的 Bt杀虫蛋白基因整合位点是在非同源染色体上
    Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection.
    通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。
    The progeny of transgenic rapeseed plants with Bt insecticidal protein gene was detected by kana mycin sifting and PCR. Results showed that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.
    通过对转 Bt杀虫蛋白基因油菜植株的后代进行卡那霉素抗性分析和 PCR技术检测 ,结果表明 :Bt杀虫蛋白基因是以单拷贝、杂合地整合到转基因植株 (T0 1、T0 2、T0 3、T0 5、T0 6、T0 7、T0 8)的基因组中 ,并稳定地遗传。
    By ELISA Bt insecticidal protein gene can efficiently express in the transgenic rapeseed plants with the 3th~9th leaf stage, and the expressing quantity is 170~260ng/25mg fresh leaf. It amounts to 0.067%~0.105% of plant soluble protein,and the insecticidal activity is 72.0%~99.4%.
    EL ISA检测表明 :在第 3~第 9叶期 Bt杀虫蛋白基因在转基因油菜植株中高效地表达 ,Bt杀虫蛋白的表达量为 170~ 2 6 0 ng/ 2 5mg鲜叶 ,占植物可溶性蛋白的 0 .0 6 7%~ 0 .10 5 % ,其抗虫效果高达 72 .0 %~ 94 .4 %。
    In this paper, vegetative( vip83 ) and crystal(cry1Ac10 and cry1Ca) insecticidal protein genes from Bacillus thuri ngiensis were simultaneously electrospored into the plasmid-free strain BMB17 1. By the means of the specific P CR detection, the recombinant strains BMB2830-171 contained cry 1Ac10 and vip83, and BMB2 882-171 had cry1Ca and vip83 , were obtained respectively.
    将构建的营养期杀虫蛋白基因vip83表达质粒pBMB2 32 8和含杀虫晶体蛋白基因(cry1Ac1 0或cry1Ca)质粒同时电转化无质粒突变株BMB1 71并双抗筛选。 经PCR特异引物扩增验证 ,分别得到含cry1Ac1 0和vip83、cry1Ca和vip83的双基因重组菌BMB2 830 1 71和BMB2 882 1 71。
    A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized.
    根据植物基因的结构特征,合成了CrylAc活性杀虫蛋白的编码序列并与内质网定位肽编码序列组成嵌合杀虫蛋白基因Bt29K。
    Studies on Transferring Insecticidal Protein Gene from Bacillus Thuringiensis into Excellent Maize Inbred-line via Microprojectile Bombardment
    以基因枪法将Bt杀虫蛋白基因导入常规玉米自交系的研究
    Transfer of Insecticidal Protein Gene from Bacillus thuringiensis into Conventional Maize Inbred-line Mediated by Agrobacterium tumefaciens
    农杆菌介导将Bt杀虫蛋白基因导入优良玉米自交系的研究
    Transfer of Insecticidal Protein Gene from Bacillus Thuringiensis into Conventional Maize Inbred-line
    利用转基因技术将Bt杀虫蛋白基因导入优良玉米自交系的研究
    Through the mediation of Nodule Agrobacterium, the insecticidal protein gene (ppip) from pseudomonas pseudoalcalgenes has been transformed into the receptor materials to develop a new transgenic forage grass with the character of grasshopper resistance.
    由于大肠杆菌的启动子不能识别类产碱假单胞菌杀虫蛋白基因(ppip),因此ppip基因不能直接在大肠杆菌细胞中表达。
    Transgenic tobacco line Kangchong 931 expressing both Bacillus thuringiensis (Bt) insecticidal protein and cowpea trypsin inhibitor (CpTI) genes was used for evaluating the insecticidal activity on Helicoverpa armigera compared with transgenic tobacco with Bt insecticidal protein gene alone. Mortality of first to third instar larvae fed on transgenic two genes tobacco for three days was significantly higher than that fed on transgenic Bt tobacco.
    以含Bt杀虫蛋白基因(单基因)烟草和常规烟草为对照,系统测定了含Bt与豇豆胰蛋白酶抑制剂(CpTI)蛋白基因(双基因)的抗虫烟草对棉铃虫(Helicoverpaarmigera)不同龄期幼虫的杀虫活性。
    To select and breed a new variety of high yield and high resistance to insects,the expression of Bt insecticidal protein gene in the transgenic rapeseed plants was studied with ELISA.
    为选育高效抗虫油菜新品种 ,采用酶联免疫吸附法检测 Bt杀虫蛋白基因在转基因油菜植株中的表达 .
    The results show that Bt insecticidal protein gene variedly expresses in the transgenic rapeseed plants with its growth stage.
    结果表明 ,Bt杀虫蛋白基因在转基因抗虫油菜植株的表达随植株生长时期不同而发生变化 .
 

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