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培养基
    A STUDY ON ANTHER MEDIA OF COMMON WILD RICE
    普通野生稻花药培养基研究
    In this study,6 media including different components were employed to analyze 6 maize(Zea mays L.)
    本研究选用了6种培养基配方对6种不同的玉米(Zea mays L.)
    MS media with BA2.0 mg/L+IAA0.2 mg/L improved callus' multiplication and differentiation.
    花药愈伤组织增殖和分化的适宜培养基为:MS基本培养基+BA2.0mg/L+IAA0.2mg/L;
    MS media with 2.0 mg/L IBA,0.4 mg/L NAA and 0.5 g/L active carbon were suitable for rooting.
    生根培养基为添加了IBA2.0mg/L、NAA0.4mg/L和0.5g/L活性碳的MS培养基
    Studied on tissue culture and rapid propagation of Ageratum houstonianum Mill. The results showed that the best explant in tissue culture was leaf. The best media for each stages were as follows: (1) Callus inducing medium: MS+6-BA1mg/L+NAA0.5mg/L;
    对藿香蓟组培快繁技术进行了研究,结果表明:叶片为组织培养的最佳外植体,各培养阶段适宜培养基为:诱导愈伤组织培养基:MS+6-BA1mg/L+NAA0.5mg/L;
    Callus was induced using seeds as explant on Dioscorea nipponica Makino,the best culture media is MS+6-BA3.0 mg/L+NAA2.0 mg/L and the highest frequency is 18.9%.
    以穿龙薯蓣种子为外植体诱导出了愈伤组织,最佳培养基为MS+6-BA3.0mg/L+NAA2.0mg/L,最高出愈率为18.9%;
    The results showed that NB was optimal media of callus induction on immature embryos in different maize inbred lines, concentration of 2,4-D 2 mg/L could obviously enhance inducing frequency, concentration of sucrose 20 g/L and 20 g/L glucose or sucrose 30 g/L and 10 g/L glucose was the better conditions of callus induction, but could not be used in long period.
    对于不同基因型玉米自交系2,4-D浓度为2mg/L时胚性愈伤组织诱导率均最高; 在以20g/L蔗糖和20g/L葡萄糖及30g/L蔗糖和10g/L葡萄糖作碳源的诱导培养基上愈伤组织诱导率最高,但不能长期继代使用。
    3. The results showed that using the modified corn media with 2 mg/L, KT 0.2 mg/L, CaCl2 5 mmol, Pro 600 mg/L and CH 500 mg/L as initiation medium, 78599 easy to be initiated the callus.
    结果表明:用改良的玉培培养基+2mg/L2,4-D+0.2mg/LKT+5mmol CaCl2+600mg/L脯氨酸+500mg/L水解酪蛋白+7g/L琼脂+30g/L糖进行诱导培养,3种基因型中78599最易诱导出愈伤组织。
    In both solid and liquid media, 1 mg·L-1 2,4-D supplement was suitable for embryogenic callus multiplication.
    而且1mg. L-12,4-D均适合于固体和液体培养基中胚性愈伤组织的增殖;
    Differentiation rate of callus induced on the media with low concentration of 2,4-D was higher than that from the media with high concentration.
    诱导培养基中低浓度的2,4-D有利于所诱导的愈伤组织的分化。
    The results indicated that the strong and green clump-buds could be induced on the MS media containing BA0.2mg/L,IAA0.05mg/L and NAA0.2mg/L.
    确定了杭白菊最佳繁殖培养基为MS+BA(6-苄基嘌呤)0.2 mg/L+IAA(吲哚乙酸)0.05 mg/L+NAA0.2 mg/L;
    The flower buds of 2-3 mm in length were the best explant. B5 medium with 6%-10% sucrose is the best media.
    取材以2~3 mm的花蕾效果最好,培养基则是含 6%~10%蔗糖浓度的B5培养基最好。
    Cinnamonum petrophilum grows exuberantly by Ms and wpm as basic media.
    香桂在MS.Wpm基本培养基中生长适宜。
    1/2 MS medium which could make hairy root grow longer and softer and produce more nicotine was more suitable for hairy root suspension culture than MS and 1/4 MS media.
    液体悬浮培养条件下,1/2 MS培养基中生长的发状根细长柔软,与MS、1/4MS培养基中的发状根相比,具有较高的烟碱合成能力;
    Culturing on half Strength Murashige and Skoog's(MS) medium containing 5.0mg/L 6-BA and 1.0mg/L NAA gave good result in inducing clustered shoots and the pH value of medium was kept in 5. 1-5. 4. Germinated clustered shoots grew well when cultured in half-strength liquid MS media contained 2. 0 mg/L BA for two months.
    丛生芽诱导的培养基为1/2 MS+6-BA 5.0 mg/L+NAA 1.0 mg/L,继代培养采用液体培养基1/2MS附加6-BA 2.0 mg/L,振荡培养两个月,可使其生长旺盛。
    Cultured in natural light, the pH value of culture media was 5.3-5.8, but culture media can not concrete whole on the level of 5.3, and result in reproduction inconvenience;
    日光条件下,培养基pH值适宜范围为5.3-5.8.但5.3水平培养基凝固程度稍差,接种不便;
    Cultured in lamp light, the pH value of culture media was 5.8-6.3.
    灯光条件下,培养基pH值适宜范围为5.8-6.3。
    Study on Selection of Culture Media for Polyporus umbellate
    猪苓液体培养基筛选研究
    A Preliminary Study on the Selection of Mother Culture Media of Grifola umbellata
    猪苓母种培养基筛选研究
    Experiments on the Culture Media and Compost of Ganoderma lucidum Strain G9
    灵芝G9菌种培养基配方和栽培料筛选试验
 

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