助手标题
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
共[7]条 当前为第1条到7条
 

相关语句
培养基
    Effect of macrophages conditioned media on interleukin-6 in human retinal pigment epithelial cells in vitro
    巨噬细胞条件培养基对视网膜色素上皮细胞产生白介素-6的影响
    Low Ca2+ culture media is used to culture human limbalstem cells. The cell expressions of E-cadherin are observed by immunocytochemistry.
    使用低钙(含Ca2+ 0.06mMol)培养基对人角膜缘组织进行组织块培养,并对培养出的细胞行免疫细胞化学染色,观察培养细胞E-cadherin的表达。
    Methods: Human bone marrow stromal cells line was grown on the adhesive substrate in the condition media including a-Modified Eagle medium (a-MEM) /10% fetal bovine serum.
    方法:人BMSC培养在含10%胎牛血清的(a-Modified Eagle Medium,a-MEM)培养基上扩增;
    INTERVENTIONS:HRCECs were cultured in media containing 5 mmol/L or 25 mmol/L glucose for 6 days.
    方法:HRCEC细胞在含有5mmol/L或25mmol/L葡萄糖的培养基中培养6d。
    Methods Lipopolysaccharide(LPS) activated and non activated human peritoneal macrophage conditioned media (MφCM) in three different concentrations were administered to purified cultured hRPE cells for 24,48 and 72 hours. The OD values of RPE cells were measured by MTT colorimetric assay.
    方法制备脂多糖(Lipopolysaccharide,LPS)激活的和未激活的人腹腔巨噬细胞条件培养基(MφCM),取3种不同的浓度,分别施加于纯化培养的胎眼RPE细胞,作用24、48、72h,用MTT比色法测定RPE细胞的OD值。
    Objective The study was designed to investigate the effect of arsenic trioxide (As 2O 3) on metastasis of nasopharyngeal carcinoma cell line HNE1 LMP1.Methods HNE1 LMP1 cells were cultured in the media containing 3 μmol/L of As 2O 3 for 48 hours and then recovered in the free drug media for following 48 hours.
    目的 观察三氧化二砷(As_2O_3)对人鼻咽癌细胞株HNE1 LMP1 侵袭、转移的影响。 方法 鼻咽癌细胞在含3μmol/L的As_2O_3培养基中培养 48 小时,然后在无含砷的培养基中继续培养 48 小时后,收集此时间点贴壁的细胞作为研究对象;
    Methods The third-forth passage human RPE were treated with TGF-β_(1) at different concentrations(0.01,0.1,1,10 ng/ml),and the conditioned media were collected after 36 h,or for different time(24,36,48 h),and the conditioned media were collected. The activities of MMP-2 in human RPE supernatant were quantitatively determined by gelatin zymography.
    方法3~4代培养的人视网膜色素上皮细胞,经不同浓度的TGF-β1(0.01、0.1、1、10 ng/ml)处理,36 h后收集条件培养基,或经TGF-β1作用不同时间(24、36、48 h)后收集条件培养基,采用明胶酶谱分析方法定量检测HRPE细胞上清液中MMP-2的活性水平。
 

首页上一页1下一页尾页 

 
CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社