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    The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively.
    设计3对表达引物分别扩增侵染了百合无症病毒(lily symptomless virus,LSV),百合斑驳病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外壳蛋白基因,并在大肠杆菌BL21(DE3)plysS中原核表达。
    [OBJECTIVE]Fast identification and detection of Cucumber green mottle mosaic virus (CGMMV)is the key way to control its further spreading.
    【研究目的】黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的快速鉴定检测是控制该潜在危险性有害生物蔓延的有效途径。
    A multiplex RT-PCR method was developed to simultaneously detect three viruses of Lily by using 3 sets of specific primers designed according to CMV(Cucumber mosaic virus),LMoV(Lily mottle virus)and LSV(Lily symptomless virus).
    根据基因库中黄瓜花叶病毒(Cucumber mosaic virus,CMV)、百合斑驳病毒(Lily mottle virus,LMoV)、百合无症病毒(Lily symptomless virus,LSV)的外壳蛋白基因序列,设计了3对特异引物,通过对扩增条件进行优化,建立了同时检测CMV、LMoV和LSV的多重RT-PCR检测方法。
    Identification and detection of pathogen causing mottle leaf of pomelo
    柚类果树叶片斑驳病的病原鉴定与检测
    Antiserum Preparation of Carnation Mottle Virus in Hunming and Its Application
    香石竹斑驳病毒的抗血清制备及应用
    Identification and detection of carnation mottle virus on carnation by RT-PCR
    香石竹斑驳病毒的鉴定和RT-PCR检测
    Production of monoclonal antibodies to Carnation mottle virus and it s application in virus detection
    香石竹斑驳病毒单克隆抗体的制备及检测应用
    DETECTION OF BEAN POD MOTTLE VIRUS BY RT-PCR
    菜豆荚斑驳病毒的RT-PCR检测
    RT-PCR detection of Strawberry mottle virus based on internal control
    利用内标为基础的RT-PCR技术检测草莓斑驳病毒
    Detection of Lily symptomless virus and Lily mottle virus by Multiplex RT-PCR
    应用多重RT-PCR检测百合无症病毒和百合斑驳病毒
    Detection of Strawberry mottle virus and Strawberry mild yellow edge virus by Multiplex RT-PCR
    应用多重RT-PCR检测草莓斑驳病毒和草莓轻型黄边病毒
 

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