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正常口腔
    The Distribution of Normal Oral Flora in 49 Healthy Children and Juvenile
    49例健康儿童和青少年正常口腔菌群的分布
    Methods The expression of EGFR was observed in 10 cases of normal oral mucosa, 16 cases of normal OLP,10 cases of OLP with dysplasia and 14 cases of oral squamous cell carcinoma(OSCC) by immunohistochemical evaluation.
    方法采用免疫组化法检测10例正常口腔黏膜,16例扁平苔藓,10例扁平苔藓伴不典型增生,14例口腔鳞癌上皮组织中表皮生长因子受体(EGFR)的表达水平。
    Methods: SP immunostaining technique was used to examine the expression of TGF-β1,smad4 and cyclinD1 in oral squamous cell carcinoma and normal oral mucosa.
    方法:采用免疫组化SP法分析TGF-β1、smad4、cyclinD1在正常口腔黏膜和口腔鳞癌组织中的表达情况。
    Results: TGF-β1 expressed positively in 35.7% of normal oral mucosa while 69.0 % of oral squamous cell carcinoma(P<0.05).
    结果:TGF-β1蛋白阳性表达率在正常口腔黏膜组织为35.7%,口腔鳞癌组织中为69.0%(P<0.05);
    Methods:The expression of TNF-α was assessed by immunohistochemical method of SABC in paraffin tissue sections of 41 cases of OSCC and 10 of normal oral epithelia.
    方法:应用免疫组化SABC法检测41例口腔鳞癌组织中TNF-α的表达,并用10例正常口腔黏膜组织作为对照。
    Results The positive rate of PTEN protein was 72.2%(52/72)、93.3%(14/15)、100%(10/10) and 100%(10/10) in OSCC,oral leukoplakia,oral epithelial hyperplasia and normal oral mucosa,there was significant correlation among them(P<0.05).
    结果PTEN基因在口腔鳞癌、口腔粘膜白斑、口腔上皮单纯增生和正常口腔粘膜组织中的阳性率分别为72.2%(52/72)、93.3%(14/15)、100%(10/10)和100%(10/10),口腔鳞癌组与其他各组间有显著性差异(P<0.05);
    Results: Positive expression ratios of Hpa and Hpa mRNA in leukoplakia were significantly higher than those in normal oral mucosa, while were significantly lower than in OSCC(P<0.05). Muscle-invading OSCC cells showed higher levels of Hpa mRNA and protein than those of mucous-invading and submucous-invading OSCC(P<0.05).
    结果:在口腔白斑组织中Hpa蛋白、Hpa mRNA的阳性表达率均显著高于它们在正常口腔黏膜组织中的表达(P均<0.05),并显著低于它们在OSCC中的表达(P均<0.05)。
    MethodsThe expression of hMLH1and hMSH2 in 69 cases of OSCC and 40 normal oral mucosa was examined by immunohistochemical methed. The data was analyzed by Chi-square test.
    方法采用免疫组化法检测69例口腔鳞状细胞癌和40例正常口腔粘膜组织中hMLH1、hMSH2的表达情况,资料的统计学处理用SPSS软件完成,比较用卡方检验。
    ResultsPositive rates of hMLH1 and hMSH2 were lower in OSCC(47.8% and 55.1% respectively)than in normal oral mucosa(77.5% and 80.0% respectively). These differences between OSCC and normal oral mucosa were statistically significant(P<0.05).
    结果口腔鳞状细胞癌组织中hMLH1、hMSH2的阳性表达率(47.8%,55.1%)低于正常口腔粘膜组织(77.5%,80.0%),P<0.05,有显著差异。
    Methods Immunohistochemical staining with SABC method and methyl green-pyronine staining were used to detect the expressions of Ki-67 in 30 cases of OLP and 20 cases of normal oral mucosa specimens (the controls).
    方法采用甲基绿-派诺宁染色法及SABC免疫组化方法检测30例OLP及20例正常口腔黏膜(对照组)中Ki-67蛋白表达及细胞凋亡情况。
    Methods Expression of VCAM-1 and CD31 in 48 cases of OSCC and 10 cases of normal oral mucosa was detected by immunohistochemistry.
    方法用免疫组化二步法检测48例口腔鳞状细胞癌组织和10例正常口腔粘膜组织中VCAM-1和CD31表达并计数微血管密度(MVD)。
    VCAM-1 was detectable in 31 out of 48(64.58%)cases of OSCC and none in 10(0.00%)cases of normal oral mucosa(P<0.01).
    VCAM-1在口腔鳞状细胞癌组织中的表达率显著高于正常口腔粘膜组织(P<0.01);
    OBJECTIVE: To study the relationship of the expression of new apoptosis-related gene TFAR19 and p53 in normal oral mucosa, oral leukoplakia and oral squamous cell carcinoma.
    目的:研究凋亡相关新基因TFAR19与p53在正常口腔黏膜、口腔白斑和口腔鳞癌中表达的相关性及其意义。
    METHODS: The expression of TFAR19 and p53 was observed separately in 17 specimens of normal oral mucosa, 60 specimens of oral leukoplakia, and 30 specimens of oral squamous cell carcinoma by immunohistochemical assay.
    方法:采用免疫组织化学方法检测17例正常口腔黏膜、60例口腔白斑、30例口腔鳞癌组织标本中TFAR19及p53的表达。
    METHODS: Immunohistochemical staining was used to detected the expression of c-myc and hTERT protein in 48 cases of OLK, 41 cases of OSCC and 10 cases of normal oral mucosa organizations.
    方法:采用免疫组化方法检测48例OLK、41例OSCC和10例正常口腔黏膜组织中c-myc蛋白和hTERT蛋白的表达,并进行统计学分析。
    In the comparisons between the numbers of infiltrating mast cells (five 400 sights)in the oral lichen planus (51.00±11.41 )and in the normal oral mucosal tissue (39.89±5.99),there were significant differences (P<0.05).
    口腔扁平苔藓黏膜固有层肥大细胞的数量为(51.00±11.41)个细胞/5个视野,与正常口腔黏膜(21.21+4.27)个细胞//个视野比较,差异有显著性(P<0.05)。 TNF-α的表达与肥大细胞的数量具有相关性(r=0.399,P<0.05)。
    METHODS: Immunohistochemicalassay and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 oral lichen planus cases and 10 normal oral mucosa specimens.
    方法:采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、免疫组化技术检测50例口腔扁平苔藓及10例正常口腔黏膜中TNF-α、肿瘤坏死因子受体I(TNFRI)的表达及细胞凋亡情况。
    Expression and subcellular localization of XIAP and XAF1 in human normal oral keratinocytes and Tca8113 cells
    XIAP、XAF1在正常口腔黏膜角化细胞和Tca8113细胞中的表达与定位
    The Expression and Localization of XIAP and XAF1 in Human Normal Oral Keratinocytes and Tca8113 Cells
    XIAP与XAF1在正常口腔黏膜角质形成细胞和Tca8113细胞中的表达与定位
    Objective: By observing and analyzing the different expressions of E-cad MMP-9 or CD44V6 at normal oral mucous oral atypical hyperplasia and oral squamous cell carcinoma(OSCC), to investigate the relationship of their abnormal expressions cancerate and metastasis.
    目的:通过观察E-Cadherin(E-cad)、MMP-9和CD_(44)V_6在正常口腔粘膜、口腔粘膜不典型增生和口腔粘膜鳞癌的不同表达,分析它们在口腔粘膜鳞癌及其癌变过程中的表达规律,探讨它们的异常表达与口腔粘膜癌变、转移的关系。
 

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