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    According to the specific sequence of DHBV P genes, the primers and fluorescent probe were designed and synthesized .
    根据鸭乙型肝炎病毒(duck hepatitis B virus,DHBV)P基因上的保守序列设计并合成引物和荧光标记探针,建立了荧光定量PCR检测方法。
    Cloning and Identifying of P Gene of Newcastle Disease Virus F_(48) E_9 Strain
    新城疫病毒F_(48)E_9株P基因的克隆及鉴定
    ClONING OF NP, P AND L GENE OF NEWCASTLE DISEASE VIRUS OF GOOSE ORIGIN AND IDENTIFICATION OF P GENE EXPRESSION
    鹅源新城疫病毒NP、P和L基因的克隆与P基因的表达鉴定
    Cloning and sequencing of NP gene,P gene and intergenic region in NP and P gene coding region junction of Newcastle disease virus V4 strain
    新城疫病毒V4株NP和P基因及其间隔区序列的克隆与分析
    Pathological Examination of the Chick Embryo Inoculated by NDV F_48E_9 Strain and Cloning,Expression of P Gene of NDV F_48E_9 Strain
    新城疫病毒F_48E_9株接种鸡胚的病理学观察及其P蛋白基因的克隆与表达
    The genomes of SF02 isolate and of NDV strains contain 6 common ORFs in the same order: 3' -NP-P-M-F-HN-L- 5', but SF02 genome has extra 6nt between NP and P genes. Moreover, an antisense ORF at the 1960 to 1412 is found in the genome of SF02, but it is absent in NDV genomes.
    全基因组与NDV一样,都由3' -NP-P-M-F-HN-L- 5'六个ORF组成,但在SF02基因组的1960-1412位还存在有一个反义ORF,该ORF在序列已知的NDV毒株中并不存在。
    The total RNA in cartilage cells was extracted, IGF- I -cDNA and TGF-β -cDNA were amplified, being ligated with PMD-18 vector, and the expression of IGF-I gene mRNA and TGF-P gene mRNA was measured with the RT-PCR.
    PCR产物经琼脂糖凝胶电泳,用β-actin作内参,用凝胶成像分析仪检测IGF-Ⅰ mRNA、TGF-β mRNA的相对表达量。
    In this study, the cDNA fragments containing phosphoprotein(p) gene and matrix mrotein(M) gene of three rabies virus strains in Guangxi were amplified by reverse transcription-polymerase chain reaction(RT-PCR) with primers NSM1/NSM2, respectively.
    本试验设计引物NSM1/NSM2对广西三株狂犬病病毒P和M基因同时一起进行了RT-PCR扩增、克隆和测序。
    The nucleotide homology in P gene ORF among three rabies virus strains inGuangxi were 87.2% (GXBM/GXLA), 98.4% (GXBM/GXNIU) and 87.5%(GXLA/GXNIU) and in M gene among these three street strains were 90.1%(GXBM/GXLA and GXLA/GXNIU) and 99.7%(GXBM/GXNIU), which aremuch high;
    广西三株野毒之间核苷酸同源性较高,P基因核苷酸同源性分别为87.2%(GXBM/GXLA),98.4%(GXBM/GXNIU)和87.5%(GXLA/GXNIU),M基因核苷酸同源性分别为90.1%(GXBM/GXLA和GXLA/GXNIU)和99.7%(GXBM/GXNIU);
    69.7% to 71.0% in P gene, and from 82.8% to 87.8% or from 75.0% to 77.8% inM gene, respectively.
    M基因的分别为82.8%—87.8%和75.0%—77.8%;
    a/P genes were digested and ligated with dual expression vector pVITRO-2 using restriction endonucleases and DNA ligase.
    α、β基因与载体pVITRO-2用限制性内切酶酶切、DNA连接酶连接,成功构建山羊FSHα、β双表达载体pVITRO-FSHαβ。
    IFNa/p gene encoding mature protein was subcloned into pGEX-KG and transformed BL21 (DE3) bacteria.
    在此基础上,通过PCR方法将编码成熟蛋白基因亚克隆到PGEX-KG表达载体,转化宿主菌BL_(21)(DE_3),经IPTG诱导后,得到高效表达,其表达的蛋白质大小约47kd。
    The sequences of three aldolase isozyme genes ( ALDOA, ALDOB and ALDOC) have been deposited in GenBank database with the respective accession numbers: AY359812, AY359813 and AY359811. The other genes isolated were selenoprotein P gene (SEPP1) and membrane protein expressed in epithelial-like lung adenocarcinoma gene (CT120).
    将其中分离的三个醛缩酶同工酶基因(ALDOA、ALDOB和ALDOC)片段提交GenBank数据库,获GenBank收录号,分别为AY359812、AY359813和AY359811。 分离的其余两个基因为硒蛋白P基因(SEPP1)和CT120膜蛋白基因(CT120)。
    As a result,we obtained complete P gene clone of NDV F 48 E 9 strain.
    至此 ,我们获得了F4 8E9株P基因的全长克隆
    These P genes had very high homologous about 98.9%-100%. The O_1 P ORF nucleotide sequence was 100% homologous with that of the O_~78 .There were only 2 bases different between O_2 and O_1,O_~78 .
    此3株菌的P型菌毛结构基因的同源性为98.9%~100%,其中O1株和O78株的P型菌毛基因的ORF序列100%相同,O2株有2个碱基与前两者不同。
    According to the comparative analysis between the nucleotide and amino sequence of NP and P gene in NDV V4 and the counterpart in 8 other NDV strains,the homology was highest with Queenland V4. Comparison of nucleotide sequence was carried out between intergenic region in NP and P gene coding region junction of NDV V4 strain and the counterpart of other 13 NDV strains respectively.
    NDV V4株NP和P基因与其他8个NDV毒株相应部分进行同源性比较,与Quuesland V4株的同源性最高。
    The ESR and FSH P gene were proved to be the major gene that control the litter size in swine. The methods of PCR-RFLP and PCR were used to analysis the polymorphisms of the ESR and FSH P in Dongbei miii pig (36) , Junmu I hao pig (40) and Sanjianbai pig (37).
    本研究采用PCR-RFLP和PCR的方法,通过对东北民猪(36头)、军牧Ⅰ号(40头)、三江白猪(37头)3个猪群共113头母猪进行ESR和FSHβ基因的多态性分析,并采用最小二乘法分析ESR基因型、FSHβ基因型和合并基因型影响头胎和经产胎次产活仔数的遗传效应。
    The saws with the gene type AB have more litter size than that with gene types AA as well as BB gene types in ESR gene. The same results can be obtained in FSH P gene.
    在ESR基因中基因型为AB的母猪要比基因型为AA和BB的母猪产仔数高,在FSHβ基因中得到相同的结果即杂合型的母猪比纯合型的母猪产仔数高;
    Based on the genetic polymorphism of LH P gene,we try to link this candidate gene with the high productivity of Xiaoweihanyang(XWHY),to further provide scientific evidence for the basic genetic research on the XWHY'S high productivity.
    本课题以促黄体素β亚基基因的多态性为研究内容,旨在探索该候选基因与小尾寒羊高繁殖力之间的关系,为我国开展小尾寒羊高繁殖力的遗传学基础研究提供科学依据。
    Applying the powerful PCR-SSCP technique and sequence to analyze the polymorphism of LH P gene,we divide our samples into 2 groups,one group is the blood sample of 243 XWHY with littering record;
    应用PCR-SSCP技术对两组样本进行促黄体素β亚基基因序列(1686bp)多态性分析:一组为243头有产羔记录的小尾寒羊的血样;
 

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