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    The four RV P gene nucleotide homology was 83.6%-99.8%,the amino acid homology was 87.2%-99%; the sequence interacting! with LC8 was located on 143-148 amino acids remnant bases and was DKSTQT.
    四株病毒P基因核苷酸和氨基酸序列同源性分别为83.6%~99.8%和87.2%~99%,P蛋白与胞浆动力蛋白轻链LC8相互作用的序列位于143~148位氨基酸残基,均为DKSTQT,四株病毒P基因与L蛋白、N蛋白作用位点序列显示未发生影响其生物学功能的变异。
    High polymorphism at the human P gene locus in Chinese Han and Tibetan populations
    P基因在中国汉族及藏族群体中的多态性研究
    Detection of polymorphism of hepatitis B virus P gene by DNA microarray
    基因芯片检测HBV DNA聚合酶区基因多态性
    Significance of the YMDD motif mutation of P gene of hepatitis B virus
    乙型肝炎病毒P基因YMDD变异的意义
    The Construction of Recombination Baculovirus Expression Vector of Hantavirus H8205's G1P Gene Fragment and Its Expression
    汉坦病毒H8205株G1P基因片段重组杆状病毒表达载体的构建及表达
    Hepatitis B Virus P Gene's siRNA Inhibits the Expression of HBVP in Mouse
    乙肝病毒P基因siRNA对小鼠体内HBVP基因表达的抑制
    Analysis of M and P Genes and Their Coding Proteins of Four Chinese Rabies Viruses
    我国四株狂犬病毒M和P基因序列分析和所编码蛋白的分析
    Association Of Fibrinogen B P Gene Polymorphism With Plasma Fibrinogen Level In Coronary Heart Disease And Stroke
    心脑血栓性疾病纤维蛋白原Bβ链基因多态性与血浆纤维蛋白原的关系研究
    The RT-PCR product bands of TNF- a gene from EBO-WT and EBO-L97 transfected cells were identified clearly and in same level, while IFN- P gene expression did not be detected.
    RT一PCR法检测出EBO一WT和EBO一L97转染细胞内的TNF一Q基因mRNA表达,其cDNA条带强度相近,未测出IFN一p基因mRNA表达。
    ObjectionTo investigate the diversity of the mechanisms of rat GST-P gene expression regulation and the relation between rat GST-P gene expression and carcinogenesis. To search the trans-action factors binding to the enhancer element of rat GST-P (GPEI).
    研究不同化学物对大鼠GST-P基因表达的作用机理,筛选与大鼠GST-P基因强增强子元件GPEI相互作用的调控因子,探讨GST-P基因表达的调控机制及其与化学致癌的关系,为化学致癌机理的多样性、复杂性及其综合治疗和预防提供理论依据。
    Methods: P gene of HBV was amplified by one step PCR in serums beforetreatment, a year after lamivudine treatment and a year after adefovir treatment from apatient of CHB with HBV resistant to both lamivudine and adefovir, then cloned andsequenced and compared with the sequences in Genbank.
    方法一步法PCR 扩增出一例对拉米夫定和阿德福韦均耐药的慢性乙肝患者治疗前、拉米夫定治疗一年后、改用阿德福韦治疗一年后的P 基因序列,经克隆后测序,与Genbank 中已发表序列进行对比分析。
    2. In the serum one year after lamivudine-therapy, a G-to-Tchange at nucleotide 743(G743T) was found in P gene, leading to rtM204I resistant. Adouble mutation of rtP91L and rtC256S in domains A and E was also observed.
    2.拉米夫定治疗一年后HBV P 基因出现G743T 突变,导致rtM204I 变异,并伴随rtI91L、rtS256C 双突变,此外HBV 多聚酶区基因还有10 个氨基酸发生替代。
    Results:p gene,q gene and r gene frequency was 0 2292,0.2125 and 0.5683,respectively.
    结果:p基因频率0.2292,q基因频率0.2125,r基因频率0.5683。
    Methods: A pair of special primer(WU, WD) was designed to amplify a fragment of HBV DNA P gene by PCR, Other 2 similar pairs of primer (MU1, MD1, MU2, MD2) were obtained by knocking off 1 or 2 bases at the 3'-terminal of WU and WD.
    方法:在HBV DNA P基因区设计一对检测阳性率相对较高的引物(原型引物),在原型引物序列基础上将两根引物的3’末端分别截去1个或2个碱基,设计出两对参数与原型引物近似的突变引物。
    The mutation frequency of HBV P gene are 8.6% (nt528) and 9.6% (nt552 ) in chronic hepatitis B , and 0.00% (nt528/552) in asymptomatic carrier and severe hepatitis B , respectively .
    初步应用显示 :慢性乙肝组nt5 2 8和nt5 5 2位点突变率分别为 8.6%和 9.6% ,而无症状肝炎组和重症肝炎组突变率均为 0 ;
    Nt528 mutation frequency of HBV P gene are 10.5% in the patients with lamivudine treatment and 8.1% in the patients without lamivudine treatment. Nt522 mutation frequency of HBV P gene are 15.8% in the patients with lamivudine treatment and 8.1% in the patients without lamivudine treatment .
    拉米夫定治疗组与非拉米夫定治疗组比较 ,nt5 2 8突变率分别为 10 .5 %和 8.1% ,nt5 5 2突变率分别为 15 .8%和 8.1%。
    Finally, the DNA templates prepared by boiling method were used to nested PCR for amplifying segment of HBV P gene.
    并以煮沸法抽提样品为模板,进行HBV DNA P区部分片段巢式PCR扩增。
    Objective To study the heterogeneity of polymerase gene (P gene) within hepatitis B virus (HBV) genotypes based on a systematic analysis of 202 HBV P genes, providing some useful references for further studies on the relationship among HBV genotypes, P gene mutations, replication and nucleoside analogues drug-resistance.
    目的 对202株乙型肝炎病毒(HBV)聚合酶基因(P基因)序列进行系统分析,研究HBV基因型间P基因差异,为进一步研究HBV基因型、P基因变异与病毒复制及核苷类似物耐药的关系提供参考。
    Methods 202 HBV complete sequences containing P genes were obtained from GenBank and were analysed using computer softwares.
    方法采用计算机软件对GenBank中已发表的202株HBV全序列及P基因进行分析比较。
    The APAF-P gene was cut from T-APAF-P by KpnI and NheI, and then was cloned into pCATS basic, named pCAT3-APAF-P.
    以T-A克隆法,将APAF-P基因片段连入栽体pGEM-T。
 

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