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    4. The results of P16 mRNA and P16 protein using in situ hybridization and immunohistochemistry methods were closely related to the results of P16 methylation in HCC patients,HCC patients with positive P16 methylation showed negative expression of P16 mRNA and P16 protein.
    4、P~(16) mRNA原位杂交,P~(16)蛋白免疫组化证实P~(16)甲基化的肿瘤组织呈现P”帖NA阴性与P”蛋白缺失。
    2. P16 methylation were closely related to P16 mRNA and P16 protein expression in HCC patients.
    2、HCC月瘤组织 P”甲基化与 P‘’mRNA转录抑制,P”蛋白表达缺失密切相关。
    6. Western-blot assay for expression change of COX-2 cyclinD cyclinE bcl-1 bax erk erk-p before and after treatment with NS398.
    6.Western一blot实验:观察NS398对PC一3细胞的COX一、cyelinD,、cy-elinE、bel佗、bax、ERK、ERK一p蛋白表达的影响。
    Cathepsin E,Maspin,S100P was analyzed with immunohistochemical analysis ,PCR,ELISA ,Western blotting in the specimens obtained by EUS-FNA.
    免疫印迹法检测胰腺癌EUS-FNA活检物中Cathepsin E、Maspin、S100P蛋白含量。
    The expression of Cathepsin E (83.3%, p=0.029) 、 Maspin (79.2 %,p=0.023)、 S100P(91.7%,p=0.036)was observed in specimens of EUS-FNA but less in serum in cases with pancreatic carcinoma.
    胰腺癌EUS-FNA活检物中Catheps in E蛋白(p=0.029)、Maspin蛋白(p=0.023)、S100P蛋白(p=0.036)表达的阳性率都明显高于胰腺癌血清。
    The data were analyzed by software SPSS 10.0, Chi-square Test , and exact probability method were used to compare the difference of COX-2 and P53 expression between groups, Mest was used to compare the difference of MVD between groups.
    结果用SPSS10.0郑州大学2004年硕士毕业论文COX一、P”蛋白在非小细胞肺癌中的表达及与血管生成的关系统计软件分析,COX一2、P53蛋白阳性率的分析采用x’检验和确切概率法,MVD的分析采用t检验,相关分析采用直线相关分析和t检验,以。 =0.05作为统计学检验的显著性水准。
    Polyclonal antibody against S100P was generated by immunizing mice with purified S100P recombinant fusion protein.
    将纯化的S100P蛋白免疫BALB/C小鼠,制备抗S100P蛋白的多克隆抗体;
    Western blotting indicated that the antiserum against S100P could bind with the expressed recombinant protein specifically and as well as make a foundation of validate the relationship between S100P and gastric cancer.
    进而通过Western印迹,在验证抗S100P蛋白多克隆抗体特异性的同时,即可为验证S100P基因在蛋白水平上是否与胃癌的发生、进展密切相关提供研究基础。
    Objective To observe the expression differences of S100A4, S100A6, S100P mRNA in human pancreatic carcinoma, and to explore the distributions of S100 subtypes and the relationship between the subtypes and the genesis and development of pancreatic carcinoma.
    目的 观察S100A4、S100A6、S100A8、100P在胰腺癌中表达的差异,研究S100P蛋白在胰腺癌中的表达,探讨S100亚型的分布规律及与胰腺癌发生、发展的关系。
 

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