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放化纯度
    The labeled complex 131I-YP13, 131I-mPEG-YP13 and 131I-YR13 were isolated and purified by RP-HPLC. The radiochemical purity was estimated to be better than 95%.
    RP-HPLC分离纯化YP13、mPEG-YP13和对照随机十三肽YR13(YEDIKPKTSLAFR)131I标记产物,放化纯度大于95%.
    Results Antiserum affinity constant(Ka) was 1.33×1010L/mol. Iodination percentage of F protein was 34.8%,the specific activity of the product was 3548GBq/g,the radiochemical purity 92.0%,ED50(22.39±4.15)μg/L, NSB<5.0%. The sensitivity was 1.26μg/L, and the assay covered range 0~80μg/L.
    结果抗血清亲和常数Ka值为1.33×1010L/mol,F蛋白的标记率为34.8%,放化纯度为92.0%,比活度为3548GBq/g,标准曲线ED50为(22.39±4.15)μg/L,非特异性结合率(NSB)<5.0%,灵敏度为1.26μg/L;
    Fully automated synthesis of 18F-FLT was performed with the uncorrected radiochemical yield above 20% and radiochemical purity above 95% within the total synthesis time about 60 min.
    总合成时间约60 min,未校正放化产率和放化纯度分别大于20%和95%。
    Determined Method of 153Sm EDTMP Radiochemical Purity in Injection Liquor of Bone Therapeutic Radiopharmaceuticals
    骨肿瘤治疗剂注射液中 ~(153)Sm-EDTMP 放化纯度的测量方法
    Measurement of Radiochemical Purity of Na99mTcO4 with Thin_Layer Chromatography
    Na~(99m)TcO_4放化纯度的薄层色谱法测量
    Measuring of Radiochemical Purity of Myocardial Imaging Agent ~(99)Tc~mN(NOEt)_2
    心肌显像剂~(99)Tc~mN(NOEt)_2放化纯度的分析
    Analysis of the Radiochemical Purity of ~(18)F-FDG by HPLC
    HPLC法分析~(18)F-FDG放化纯度
    The Analytical of Radiochemical Purity of Tumor Receptor Imaging Agent ~(99)Tc~m-octreotide
    肿瘤受体显像剂~(99)Tc~m-octreotide的放化纯度分析
    Determination of Radiochemical Purity of ~(18)F-fluorodeoxyglucose Injection
    ~(18)FDG注射液放化纯度的测定
    (1) Sodium gluconate or tartrate were selected as chelate agent respectively, (2) The radiochemical purity was determined every an hour from Ih to 6h after labeling, (3) the pH value of acetate buffer from 3.8 to 5.8, (4) the volume of l88Re perrhenate from 50?
    (2)分别在标记后1、2、3、4、5、6h测定放化纯度; (3)乙酸缓冲液pH值从3.8至5.8;
    (2) When the keeping time of reaction was 5h, the radiochemical purity of 188Re-BSA was (91.6 ? 3.35)%, (3) When the pH value of acetate buffer was 5.0, the radiochemical purity of l88Re-BSA was (95.2 ?.6)%, (4) When the volume of l88Re perrhenate was 100?
    (3)乙酸缓冲液pH值5.0时~(188)Re标记牛血清白蛋白放化纯度达到(95.2±0.6)%; (4)淋洗液体积100μL时~(188)Re标记牛血清白蛋白放化纯度为(88.9±5.1)%;
    1. Detecting the labeling efficiency, radiochemical purity and immunoreactive fraction of 13lI-Rituximab at different reaction time (2,4,6,8rninutes) and different specific mass-radioactivity ratio (1:10, 1:15,1:20) respectively.
    1、测定不同标记时间(2、4、6、8分钟)和不同重量放射性活度比(1:10、1:15、1:20)时的标记率、放化纯度和标记物的免疫活性;
    2. Measuring the changes of radiochemical purity of 131I-Rituximab in 0-5 days at different storage conditions (stored as original solution at 4℃ or stored directly at 37℃ in CO2 fermentor with 1% hominine albumin) right after the label process.
    2、标记物以不同储存条件(原液4℃或加1%人血清白蛋白后置于CO_2孵箱37℃),在0~5天内测定标记物放化纯度的变化;
    And the radiochemical purity (92.0 ±0.35%) was significantly higher than that of 1:15(76.3 ±0.57%, P<0.01) after being kept in storage for 24 hours.
    储存24小时后放化纯度(92.0±0.35%)高于比例为1:
    131I-insulin was purified by Sephadex G15 with radiochemical purity more than 98%.
    经sePhade、G15柱分离后,13’I一胰岛素的放化纯度>98%。
    Radiochemical purity was 95.62%± 1. 78%, 94. 78%± 1. 56%(P>0.05) respectively measured by the two methods above after purification.
    经纯化后二者测得的放化纯度分别为95.62%±1.78%; 94.78%±1.56%(P>0.05)。
    As to the labeling condition: 1) when the study carried out under the temperature of 22℃ ,the highest radiochemical purity of (57.8±2.3)% was obtained.
    改变标记条件:①放化纯度随温度上升而下降,22℃时放化纯度最高,为(57.8±2.3)%,100℃时为(18.5±5.7)%。
    2) the pH value of ascorbic acid was 4.5, the radiochemical purity of 99Tcm-octreotide was 57.8±2.3%.
    ②pH为4.5时放化纯度最高,为(57.8±2.3)%。
    The radiochemical purity of labeled compound is still more than 90% after being put in 4C for 40 days.
    ‘25碘标记率为78.4%,比活度为86.34TB丫~d。分离纯化后的标记物,经40d4℃贮存放化纯度仍高于90%。
    Methyl orange was employed for the quantitative determination of [~3H] F-7302. The specific radioactivity was 59 ci/mM, and the radiochemical purity was 98%. [~3H] F-7302 binding experiments with opiate receptors of mouse brain showed a high affinity and specificity.
    反应产物经硅胶纸层析纯化后,用甲基橙比色法定量测定,得到N-{1-[β-羟基-β-氚-β-(对-氚苯基)乙基]-3-甲基-4-哌啶基}-N-丙酰苯胺(Ⅳ,[~3H]F-7302),其比放射性为59 Ci/mM,放化纯度为98%。
 

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