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    Synthesis and Cloning of the Tandem Repeated Genes for Hammerhead Ribozyme and Antisense RNA Against Melon mRNA for ACC Oxidase
    蜜瓜ACC氧化酶mRNA的成串锤头型核酶和反义RNA基因的合成和克隆
    Construction of inducible expression vector of tandem repeated genes for antifreeze peptide
    成串抗冻肽基因的诱导表达载体的构建
    IFN-γ CA short tandem repeat gene polymorphism is associated with susceptibility to intrauterine HBV infection
    干扰素γCA重复序列基因多态性与宫内乙型肝炎病毒感染易感性的关系
    A Method of Construction of Tandem Repeat Gene of Small Peptide
    一种构建多拷贝串联小分子多肽基因的方法
    The DNA sequences of rRNA repeating gene family of Tetraoden nigrovirdis were gained by bioinformatics technique of relative organism method. Based on the sequence three pairs of primers (P1/P2、 P3/P4 and P5/P6) were designed to amplify three parts of the rRNA gene from genome DNA extracted from Siniperca chuatsi. Furthermore, the PCR products were cloned into pUCm-T cloning plasmid, and sequenced.
    本研究采用亲缘生物法的生物信息学技术,获得鲀鱼rRNA重复基因家族全序列,根据该序列设计3对引物(P1/P2、P3/P4和P5/P6),以鳜鱼基因组DNA为模板,进行PCR扩增,将扩增产物克隆到pUCm-T质粒,测序。
    Result The expected amplification product, thrombospondin-1 type Ⅰrepeat gene sequence, was acquired by RT-PCR, which had a consistency up to 99% with the sequence from the GenBank.
    结果获得了预期的扩增产物———凝血栓蛋白-1Ⅰ型重复序列基因,测序结果经GenBank分析,一致性为99%。
    Objective To explore the susceptibility of children to develop intrauterine hepatitis B virus (HBV) infection through studying the association between IFN-γ CA short tandem repeat gene polymorphism and intrauterine HBV infection.
    目的 了解宫内乙型肝炎病毒(HBV)感染儿童干扰素-γ(IFN -γ)CA重复序列基因多态性与宫内HBV感染易感性的关系,探讨宫内HBV感染的遗传易感因素。
    IFN-γ CA short tandem repeat gene polymorphism was tested in both groups by capillary electrophoresis. Results IFN-γ CA short tandem repeat gene polymorphism was tested successfully. (CA_(12))~+/(CA_(12))~+ of IFN-γ CA short tandem repeat polymorphism was 11.90% in the intrauterine HBV infection group, and 26.47% in the immunocompetent children group.
    结果 在42 例宫内HBV感染组儿童中IFN- γCA重复序列基因多态性(CA12 )+/(CA12)+减少,42 例中仅有5 例,占11.90%, (CA12 )+/(CA12 )- 占26. 19%, (CA12 )-/(CA12 )- 占61.91%;
    A significant difference was found in the frequency distribution between two groups(χ~2=7.548,P=0.0060). Conclusions IFN-γ CA short tandem repeat gene polymorphism was associated with intrauterine HBV infection.
    宫内HBV感染儿童IFN γCA重复12次的频率为25%,比正常免疫儿童IFN- γCA重复12次的频率43.38%明显降低,χ2 =7.548,P=0.0060,差异有统计学意义。
    METHODS: The clostridium difficile toxin A C-terminal repeated gene was amplified by PCR and cloned into the prokaryotic expression vector pET-22b(+),and the recombined plasmid pET-CDTAR was transformed into E.
    方法:PCR扩增CDTAR基因并将其克隆到表达载体pET-22b(+),重组质粒转化到E.
    After identified by restriction endonuclease digestion analysis and DNA sequencing, MUC1 tandem repeats gene and GM-CSF gene were cloned into eukaryotic expression vector pcDNA3.1(+) to construct recombinant plasmid pcDNA 3.1(+)-MUC1-GM-CSF.
    方法:将信号肽及编码MUC1基因片段合成、合成的片段经退火等方法获得MUC1基因重复序列串联体,酶切鉴定及序列分析后,与GM-CSF基因克隆入pcDNA3.1(+)真核表达载体中,构建真核共表达质粒pcDNA3.1(+)-MUC1-GM-CSF。
    Determination of Nucleotide Sequences of Long Terminal Repeat Gene in Provirus of Eight Stains of HTLV Ⅰ and Analysis of its Subtype in Fujin and Gluangdong,China
    福建广东8株HTLV-Ⅰ前病毒长末端重复序列核苷酸序列测定及亚型分析
 

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