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    RESULTS: Compared with blank control group, the contents of monoamines transmitter in cerebral contex, hippocampus and striatum tissues of doxepin 15, 10, 5 mg·kg -1 groups and nimodipine 3 mg·kg -1 group increased significantly ( P <0.05 or P <0.01). Activity of EEG was recovered significantly ( P <0.05 or P <0.01).
    结果 :与空白对照组比较 ,多塞平 15 ,10 ,5mg·kg- 1剂量组及尼莫地平 3mg·kg- 1组大脑皮层、海马、纹状体各单胺类递质含量显著升高 (P <0 .0 5或P <0 .0 1) ,脑电活动亦显著恢复 (P<0 .0 5或P <0 .0 1)。
    AIM: To observe the effects of trihexyphenidyl (THP) on the content of monoamine neurotransmitters in the cerebral cortex and striatum tissues in rats with acute cerebral ischemia-reperfusion injury, and take nimodipine as positive control.
    目的:观察中枢性抗胆碱药苯海索对急性脑缺血再灌注大鼠大脑皮质、纹状体单胺类递质含量的影响,并与尼莫地平进行阳性对照。
    ① Content of 5-HT: The contents of 5-HT in the cerebral cortex and striatum tissues were significantly lower in the ischemia reperfusion group than in the sham-operated group (P< 0.01), but significantly higher in the trihexyphenidyl 1.5, 1 and 0.5 mg / kg groups and nimodipine group than in the ischemia reperfusion group (P < 0.01 or 0.05).
    ①5-羟色胺含量:脑皮质和纹状体缺血再灌注组均显著低于假手术组(P<0.01),苯海索1.5,1,0.5mg/kg剂量组和尼莫地平3mg/kg组均显著高于缺血再灌注组(P<0.01或0.05)。
    ② Level of norepinephrine: The levels of norepinephrine in the cerebral cortex and striatum tissues were significantly lower in the ischemia reperfusion group than in the sham-operated group (P < 0.01), but significantly higher in the trihexyphenidyl 1.5, 1 and 0.5 mg / kg groups and nimodipine group than in the ischemia reperfusion group (P < 0.01 or 0.05).
    ②去甲肾上腺素水平:脑皮质和纹状体缺血再灌注组均显著低于假手术组(P<0.01),苯海索1.5,1,0.5mg/kg剂量组和尼莫地平3mg/kg组均显著高于缺血再灌注组(P<0.01或0.05)。
    ③ Content of dopamine: The contents of dopamine in the cerebral cortex and striatum tissues were significantly lower in the ischemia reperfusion group than in the sham-operated group (P < 0.01), but significantly higher in the trihexyphenidyl 1.5, 1 and 0.5 mg / kg groups and nimodipine group than in the ischemia reperfusion group (P < 0.01 or 0.05).
    ③多巴胺含量:脑皮质和纹状体缺血再灌注组均显著低于假手术组(P<0.01),苯海索1.5,1,0.5mg/kg剂量组和尼莫地平3mg/kg组均显著高于缺血再灌注组(P<0.01或0.05)。
    MethodsBrain water content of parietal cortex and striatum tissues are measured with specific gravity (SG).
    使用比重法(SG)检测顶叶皮层和纹状体的组织含水量。
    The results showed that the content of SS in hippocampus and striatum tissues increased markedly before rats were at unconvulsion, by the time rats developed convulsion,it had a significant increase in all of the brain areas observed.
    结果显示大鼠濒临氧惊厥时海马、纹状体中SS含量显著升高,氧惊厥时所观察脑区SS含量均显著升高;
    What is the regulating effect of acupuncture on nitric oxide (NO) content and nitric oxide synthase(NOS) activity in striatum tissues?
    针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶(nitricoxidesyn-thase,NOS)活性的调整作用如何?
    ③ Observed indexes: The contents of serotonin (5-HT), norepinephrine and dopamine in the cerebral cortex and striatum tissues were determined with fluorospectrophotometry. The neurologic impairment score was observed.
    ③观察指标:用荧光分光光度计测定大脑皮质、纹状体、5-羟色胺、去甲肾上腺素及多巴胺的含量,观察大鼠神经功能缺损评分。
    ①Primary culture of neural stem cells: Corpus striatum tissues of embryonic, fetal and adult mice were isolated and blew into single cell. Cells were cultured in D/F12 medium and passaged once every 6 to 7 days.
    ①神经干细胞的原代培养:分别将孕14d小鼠、新生小鼠和成年小鼠处死后取出纹状体组织,轻轻吹打制成单个细胞悬液,接种于无血清的D/F12培养基中,每六七天机械分离克隆传代1次。
 

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