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    A Comparative Study on the Variation of Peroxidase Isoenzyme in Seveval Susceptible Plants
    几种植物感病后过氧化物酶同工酶变化的比较研究
    STUDIES ON RESISTANCE OF RICE VARIETY, NJ14,TO BROWN PLANTHOPPER,NILAPARVATA LUGENS (Stal) Ⅱ. DIFFERNT UTILIZATION OF FREE AMINO ACIDS IN RESISTANT AND SUSCEPTIBLE RICE
    水稻品种南京14号对褐飞虱抗性的研究──Ⅱ.褐飞虱对抗感水稻品神叶鞘内游离氨基酸的利用差异
    Using real-time quantitative PCR, mRNA expression levels of CYP6AE12 and CYP9A18 were compared between the resistant strain YS-FP (derived from a susceptible strain YS through laboratory selection) and the YS strain respectively.
    用实时定量PCR技术分析了这2个基因在棉铃虫YS敏感品系和YS-FP抗性品系(由氰戊菊酯加辛硫磷混剂筛选YS品系而得)6龄幼虫脂肪体和中肠中mRNA的表达水平。
    RAPD markers OPAI17-1550 and OPAI13-900,linked to anti-black-spot disease gene locus,were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13292 were validated in the resistant、susceptible pools and F1 population.
    通过测序和引物设计,将与美洲黑杨抗黑斑病基因相连锁的RAPD标记OPAI17-1550和OPAI13-900成功地转化成显性SCAR标记(SAI17-1570)和共显性SCAR标记(SAI13-292),并对感、抗病池和F1代91个无性系进行了SCAR标记检测。
    Induced-resistance effects were investigated after treated 7days on resistant cultivar and susceptible cultivar,and the results showed that the effective percentage was 30.43% and 21.21% respectively.
    处理7 d后SA在抗病品种和感病品种上表现出的诱抗效果分别达30.43%和21.21%。
    With a F2 population derived from 66 ×A18 as materials,polymorphism between resistant and susceptible bulk of cucumber anthracnose were studied using BSA method and AFLP technology. A codominant AFLP marker,E24M48-251 bp/245 bp,was screened.
    以黄瓜抗炭疽病母本66和感炭疽病父本A18及其F2代分离群体为试材,采用BSA法和AFLP技术建立了对炭疽病的抗病组和感病组,AFLP引物组合E24M48在抗感组间表现多态性,且呈共显性。
    The resistant and susceptible F2 plants possessed the 251 bp and the 245 bp fragment,respectively,and the mid-plants owned the two fragments.
    经220个F2单株分析,在高抗单株和高感单株中分别仅扩增出251bp和245bp的特异片段,而在中间类型个体中同时扩增出了该两个特异片段。
    Therefore, PCA=10(INR=5.0) might be a threshold in determining "responders" to discriminate the resistant and susceptible rodent administrated by 4.0 mg/kg bromadiolone. Conclusion BCR might be applicable method to monitor the resistance against anticoagulant rodenticides of commensal rodents.
    结论以区分剂量4.0mg/kg溴敌隆作用后,PCA=10(或INR=5.0)作为阈值来区分黄胸鼠抗药性与敏感性个体,是准确、简便、易行的抗药性测定方法。
    While in same disease grade of the resistant and susceptible inbred lines,there was no obvious difference on RBSDV concentration(F=0.775,P=0.598).
    而相同病级的抗病、感病和中抗玉米自交系叶片内的RBSDV浓度无显著差异(F=0.775,P=0.598);
    Additionally,tricin(500μg/ml) significantly inhibited oviposition and feeding of the brown planthopper females in choice tests when spread on susceptible rice plant(TN1).
    用500μg/ml的麦黄酮溶液涂抹到对褐飞虱敏感的水稻品种TN1植株上对褐飞虱雌成虫有明显的拒食作用和忌避产卵作用。
    Lambda-cyhalothrin showed LD_ 50 value of 0.9672 μg/larva at F_1 generation and decreased gradually in onward generations,resulting in LD_ 50 value of 0.0325 μg/larva at F_ 43 generation by using topical application,and the resistance level of the non-selected strain decreased from 4 836.0-fold to 162.5-fold compared with the susceptible strain.
    用点滴法测定不同世代3龄幼虫抗性结果为:室内F1代LD50值为0.9672μg/头,抗性倍数为4836.0倍,以后各世代逐渐降低,至F43代LD50值为0.0325μg/头,抗性倍数为162.5倍,抗性水平下降了29.8倍。
    In leaf dipping bioassay,lambda-cyhalothrin exhibited LC_ 50 value of 185.6 mg/L at F_1 generation and decreased gradually to onward generations with LC_ 50 value of 9.2 mg/L at F_ 43 generation. Correspondingly,the resistance level of the non-selected strain decreased from 964.7-fold to 47.8-fold compared with the susceptible strain.
    用浸叶法测定不同世代3龄幼虫抗性结果为:室内F1代LC50值为185.6mg/L,抗性倍数为964.7倍,以后各世代也逐渐降低,至F43代LC50值为9.2mg/L,抗性倍数为47.8倍,抗性水平下降了20.2倍。
    The results of this study showed that there were significant differences among the 9 lines,in which lines of 85198 and 203 were susceptible to bacterial wilt,whereas lines of 47254,51255,7585,and 85254 showed resistance to bacterial wilt.
    结果表明:这9个品系的抗性水平存在显著差异,85198和203为感病品系,47254,51255,7585和85254为抗病品系。
    Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) was employed as tools to detect molecular markers linked to Fusarium wilt resistant gene in the watermelon wild germplasm (Citrullus lanatus var. citroides). From screening the Fusarium wilt resistance of F_2 population consisted by the introgression of Fusarium wilt resistant gene from PI296341 into the susceptible inbred 97103 by using root- dip inoculation, the pools of Fusarium wilt resistance and susceptibility were estabhshed.
    运用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)与简单重复序列(Simple sequence repeat,SSR)技术,采用混合分组分析(bulked segregant analysis,BSA)方法进行了西瓜野生种质(Citrullus lanatus var.citroides)PI296341抗枯萎病基因连锁的分子标记研究.
    The results indicated: the mainly physiological races derived from tomato leaf mold are race 1,2,3. cf0,cf1 ,cf2 and cf3 are higher susceptible genes, cf4 is higher resistant gene and cf5, cf9 are immune gene.
    结果表明:辽宁省番茄叶病生理小种分化是以1、2、3为主。 cf0、cf1、cf2、cf3为高度感病基因,cf5、cf9 为免疫基因。
    Virus-inoculation results in Tm -22 transgenic tobacco showed the expression of Tm -22 gene was single-copy integration and inherence, in which encoded protein displayed resistant to isolate TMV, ToMV-0, ToMV-2 and susceptible to ToMV-2a.
    转Tm-22基因烟草接种的结果表明,Tm22基因在烟草上的表达为单基因整合和遗传,其编码蛋白对TMV、ToMV-0和ToMV-2三个毒株的分离物表现抗性和对ToMV-2a毒株的分离物表现感病,与Tm-22番茄对ToMV病毒不同株系的特异反应结果一致;
    Susceptible Helicoverpa armigera (Hubner) strain was used as the insect hosts to study the effect of transgenic Cry1 A+CpTI cotton on the development of the parasitoid, Microplitis mediator (Haliday).
    以多年饲养的敏感棉铃虫为寄主,在室内研究了转Cry1A+CpTI双价基因抗虫棉对中红侧沟茧蜂的影响。
    Successive generation screenings were conducted for the resistance of striped stem borer Chilo suppressalis (Walker) to cry1Ab (after 10 generations selection, cry1Ab-R) and cry1Ac (after 7 generations selection, cry1Ac-R) reared on synthetic diets separately containing sublethal dosages of the two toxic proteins. The results showed that, in comparison with the susceptible strain (reared on the diet without toxic protein), the resistance of C.
    取食分别用含亚致死剂量的cry1Ab和cry1Ac毒蛋白的人工饲料饲喂二化螟(Chilo suppressalis)进行继代抗性筛选,cry1Ac筛选了7代、cry1Ab筛选了10代后,与敏感品系相比,cry1Ab-R筛选品系对 cry1Ab的敏感性下降1.54倍,cry1Ac-R筛选品系对cry1Ac的敏感性下降3.38倍。
    as fed on cry 1 Ab diet, the larval survival of cry 1 Ab- R slrain was much more higher than that of susceptible strain, while there was no significant difference between the larval survivals of cry 1 Ac- R strain and susceptible strain, as fed on cry1 Ac diel.
    cry1Ab-R筛选品系取食含cry1Ab的饲料后,幼虫存活率与敏感品系相比明显增加,但取食含 cry1Ac饲料后,cry1Ac—R筛选品系与敏感品系的幼虫存活率差异不显著。
    RFLP Analysis of Wheat Varieties Resistant or Susceptible to Scab
    抗、感小麦赤霉病品种RFLP的初步分析
 

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