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    These replicase genes were cloned into plant expression vector pBI121. PCR and double digestion confirmed that the recombination plasmids contain the target genes.
    将这些目的基因片段定向克隆到植物表达载体pBI121中,PCR及酶切鉴定重组质粒正确。
    Southern blot analysis and PCR of target segment in the genome of tobacco showed that all of the plants generated were the transgenic plants.
    (4)转基因植株总DNA PCR和Southern blot分析表明目的基因已经整合到植物基因组中,但转基因的拷贝数与抗病性无明显的相关性。
    The results showed that the RNA quality extracted from the Trizol regeat accorded with the demands of RT-PCR amplification, and every target amplification was the same as expected.
    结果表明,模板RNA提取质量好,符合RT-PCR扩增要求,5个病毒基因模板RNA对每个目的基因的扩增都与预期结果一致,说明RT-PCR反应体系正常,对每个目的片段的直接测序结果与设计的引物扩增片段序列相符。
    As a result, the molecular weight of the target protein is 36kD, which is consistent with the deduced one from nucleotide sequence, 33540.5 daltons.
    经SDS—聚丙烯酰胺凝胶电泳检测,表达的目的蛋白的分子量为36kD,与由DNA序列推导的蛋白分子量(33540.5 daltons)基本相符。
    Acetolactate synthase (ALS) is the main target of herbicides and bispyribac sodium prevents biosynthesis of branched chain amino acids by inhibition of ALS.
    乙酰乳酸合成酶 (ALS)是除草剂主要作用靶标 ,新型除草剂农美利 (bispyribac sodium)即是通过抑制ALS的活性使支链氨基酸的生物合成受阻而达到除草目的
    The result of phage plaques bright selection experiment showed that the titers of the two cDNA libraries got to 4.2×10~6 and 3.1×10~6 mL~(-1) respectively,indicating that the libraries were of high quality for obtaining target genes.
    经LE392菌株平皿测定,两文库的库容量分别为4.2×106和3.1×106mL-1,包含了绝大多数低丰度的cDNA,表明所构建的文库达到了用于分离筛选目的基因全序列的建库要求。
    The results showed that the pattern of "65 % target trees+ 25 % non-host trees+ 10 % bait trees" was the best and the threshold value was 0.948,which can resist A. glabripennis disaster and assure optimized integrated benefit.
    研究结果表明:“65%目标树种+25%非寄主树种+10%诱饵树”模式的综合效益最佳,评价决策值为0.948,既达到了抗御光肩星天牛灾害的目的,又保证了综合效益的最大化;
    the positive rates were 9.6% and 0.AFLP primer pair E20M64 was found closely linked to target gene,genetic distance was 4.83 cM.
    在F2群体中找到了1个AFLP标记E20/M64,与目的基因的遗传距离是4.83 cM;
    SSR primer CSWCT02B was linked to target gene with genetic distance of 28.7 cM.
    1个SSR标记CSWCT02B,与目的基因的遗传距离是28.7 cM。
    The authors detected GLRaV-3 by immunocapture reverse transcriptase polymerase chain reaction(IC-RT-PCR) through the following steps from capturing virus,releasing RNA,transcribing cDNA to producing the target fragments of about 300bp.
    结合ELISA和反转录聚合酶链式反应(RT-PCR)的特点,引入了另外一种方法,即免疫捕捉反转录聚合酶链式反应(IC-RT-PCR)对葡萄卷叶病毒Ⅲ(GLRaV-3)进行了检测。 从免疫捕捉病毒、释放RNA到反转录成cDNA,最后扩增出了约300bp的预期目的片段。
 

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