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    An Experimental Study of Hypoxia Inducible Factor-1α and Its Target Genes Expressed in Spinal Injury
    Various reaction factors influencing the yield of the target product were discussed. The optimum reaction conditions were as follows:the molar ratio of O-chloronitrobenzene, hydrazine hydrate to PEG-800 was 1∶4∶0.03, the speed of agitator 500 r/min,the reaction time 5 h,the reaction temperature 110℃.
    coli BL21(DE3) and induction by IPTG and SDS-PAGE indicated that the target protein was expressed.
    转化宿主菌BL21(DE3),经IPTG诱导、SDS-PAGE分析表明目的蛋白得到表达; 且能与His-Taq单抗相结合。
    Results: The recombinant plasmids of pET-22b(+)-CYP2 B6,pET-28b(+)-CYP2 B6,and pET-32a(+)-CYP2 B6 were obtained and identified by PCR and sequencing. Commassie Blue staining method was used to detect the target protein expression.
    采用考马斯亮蓝染色法检测重组蛋白表达,pET-22b(+)-CYP2 B6未见目的蛋白表达;
    The seven plasmids were transfected into SP2/0 cell in vitro to detect the expression of target genes.
    Target genes are sieved from cDNA libraries,and are used directly for expression.
    Objective To construct stable RNA interference expression vector of human neuropathy target esterase(NTE) on the universal eukaryotic expression vecter.
    Objective To study the acute toxicity and the main target organs of subchronic exposure to hydroxylammonium nitrate(HAN).
    目的研究硝酸羟胺(hydroxylammonium nitrate,HAN)的急性毒性及亚慢性染毒大鼠后主要毒作用的靶器官。
    Routine genetic engineering technique was applied to construct the prokaryotic expression systems of genotypes ompL1/1 and ompL1/2, and Ni-NTA affinity chromatography was performed to extract the target recombinant products rOmpL1/1 and rOmpL1/2. Immune aurosol electron microscopy was selected to locate the position of OmpL1s on leptospiral envelope.
    采用常规基因工程技术构建ompL1/1和ompL1/2主要基因型原核表达系统,Ni-NTA亲和层析法提纯目的重组表达产物rOmpL1/1和rOmpL1/2。 采用胶体金免疫电镜技术,对OmpL1s进行膜定位。
    Objective:To evaluate the method of CR molybdenum target in breast lesions an d the factors of influence in take high quality imaging.
    CONCLUSION: The constructed cDNA library is up to the standard and suitable to be used to screen target cDNA clones.
    AIM: To decide the effect that selected siRNA degrades mRNA of IL-1α specifically and suppression of its expression after connected with target site with homology complementary sequence.
    pMSCV-mHCN4-EGFP and pMSCV-EGFP retrovirus vectors were constructed by enzymatic reconnection of target segments from vectors pUC18, pcDNA3-mHCN4, pIRES2-EGFP and pMSCV-puro.
    Objective To determine the potential value of ~(18)FDG PET-CT on target volume defini- tion of metastatic lymph nodes in patients with advanced esophageal carcinoma.
    目的分析~(18)FDG PET-CT诊断食管癌淋巴结转移的优势及确定淋巴结放疗靶区的可行性。
    Objective To construct the target siRNA vector to peroxisome proliferator-activated receptor-γ( PPAR-γ).
    Objective:To investigate the relationship between serum levels of C-respective protein(CRP) and matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 and acute coronary syndrome(ACS) and its significance as serum target of ACS.
    To construct the target cells that carry Ag85B antigen of Mycobacterium tuberculosis for the evaluation of the function of cytotoxic T lymphocytes(CTL) induced by vaccine for tuberculosis,the recombinant plasmid pTB30s carrying ag85B gene was transfected to mouse myeloma cell line SP2/0 cells with lipofectamine,and the positive clones were cloned with high concentration of G418.The transcription and expression levels of ag85B gene were identified by RT-PCR and immunohistochemical stain.
    目的构建表达结核分枝杆菌分泌蛋白Ag85B的靶细胞以便评定结核疫苗的特异性细胞毒(CTL)作用。 方法将携带有Ag85B基因的重组质粒pTB30s转入小鼠骨髓瘤细胞(SP2/0),用RT-PCR及免疫组化染色法鉴定阳性克隆细胞胞内的Ag85B基因转录和蛋白表达水平;
    The aim,target,subjectivity,objectivity,types,methods,steps and evaluation of specialty setting for peasant employment training are introduced in detail.
    The purity of target protein could reach 95% after purification.
    The genetic transformation of plants via agrobacterium tumefaciens is a natural transgenic system,with which the exogenous DNA is transferred to target plant by processing and transfering the DNA of expression vector.


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