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靶rna
    Computer analysis of the interaction of ribozyme and target RNA
    抗HPV16E7-ribozyme和靶RNA相互作用的计算机分析
    We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program. The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme.
    应用计算机分析了抗HPV16E7-ribozyme的体外切割反应结果,发现ribozyme和靶RNA的二级结构及其能量变化能影响ribozyme的切割活性。
    After in vitro transcription, the cleavage reaction was shown to have cut off 79.3% target RNA in 1h.
    经体外转录出核酶RZ1及靶RNA后,体外切割反应1小时,79.3%的靶RNA即被切开。
    Objective To study the in vitro cleavage of mdr1 target RNA by ribozyme and its influencing factors.
    目的 探讨体外核酶对肿瘤多药抗性相关基因mdr1靶RNA分子的切割作用及其影响因素。
    The 32 P labled caspase 3 transcript being target RNA, cleavage reaction in vitro showed that pM1 GS716 and pM1 GS337 were active, the extent of pM1 GS716 cleavage was 93%.
    3 2 P标记的caspase 3基因片段体外转录物作为靶RNA ,体外切割实验表明 ,pM1 GS716和pM1 GS337均有切割活性 ,其中pM1 GS716的切割效率可达到 93% .
    Conclusions The anti-PDGFR- β ribozyme can be expressed stably in HSCs, cleave the target RNA effectively, inhibit HSCs proliferation and collagen synthesis, and induce HSC apoptosis.
    结论 抗PDGFR-β核酶的真核表达载体可在细胞内稳定表达,能有效切割靶RNA,抑制HSC增殖及胶原合成,并诱导其凋亡。
    Objective To investigate whether specific deoxyribozymes aimming at hepatitis C virus(HCV)5′-noncoding region(5′-NCR)have efficient inhibition effect on the target RNA in transgene HepG2.9706 cells.
    目的 观察特异性脱氧核酶 (DRz)在丙型肝炎病毒 (HCV) 5′ 非编码区 (5′ NCR)转基因肝癌细胞株中对靶RNA的抑制活性。
    Methods The fragments of the second exons of pro alpha 1Ⅰand Ⅲcollagen genes were cloned in-to the plasmids pT-Ⅰand pT-Ⅲand labeled with 32 P during transcription to obtain the target RNA.
    方法将含α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段的重组质粒(pT-Ⅰ、pT-Ⅲ),经体外32P标记转录后形成产物靶RNA
    CLEAVAGE ACTIVITY OF RIBOZYMES ON A TARGET RNA OF TOBACCO MOSAIC VIRUS IN E. coli
    核酶在大肠杆菌内对烟草花叶病毒靶RNA的切割作用
    PART I Selection of Binding Sites and Design of Specific DZ against Both Subgroups of RSVThree main basic factors should be considered for DZ cleavage site selection against the target RNA.
    靶RNA中DZ切割位点的选择主要考虑三个因素。
    Anti-sense RNA is a micromolecular transcript which can bind target RNA by base complementation and restrain its function.
    反义(anti-sense, AS)RNA是反义核酸技术的一种,是指能够通过碱基互补与靶RNA(主要是mRNA)特异性结合,抑制靶RNA的功能,从而控制其表达的一类小分子转录物。
    ammerhead ribozyme recognizes and cleaves target RNA by base pairing to sequences of both sides ofcleavage site on target RNA.
    锤头结构Ribozyme(Rz)通过与靶RNA上切卢、二狈帕5核普酸序列的碱基配对识别和切割靶RNA
    According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system.
    根据锤头结构ribozyme的能量变化模型,对抗HPV16E7-ribozyme和靶RNA在痘苗表达体系及稳定表达体系中的相互作用,进行计算机模拟分析。
    Methods: The c-erbB-2 mRNA was taken as the target RNA. Ribozymes were designed according to the "hammerhead structure" described by Symons.
    方法:根据Symons的“锤头结构”,以人癌基因c-erbB-2 mRNA为靶RNA,用计算机对其可能为核酶切割的位点进行分析以寻找最佳切点;
    METHODS Three single ribozymes against hepatitis B virus core gene were designed with computer, then transctiptional vector of three singleribozymes were constructed, HBV protein influence on the cleavage activity of single ribozyme on target RNA observed, and HBV protein interaction with target RNA further analysed.
    方法 利用计算机辅助设计针对乙型肝炎病毒 C基因的三个单一核酶构建 Rz1核酶自剪切转录载体 (p GEMRz1) ,观察单一核酶 (Rz1)对靶 RNA的切割作用及乙型肝炎病毒蛋白对核酶剪切作用的影响 .
    RESULTS Single antiviral ribozyme(Rz1) could cleave its target RNA efficiently, HBV proteins had no effects on ribozyme mediated cleavage on HBV. Further results indicated that HBV proteins did not interact with target RNA in our experiment.
    结果 构建的核酶自剪切转录载体体外转录后 ,在顺式核酶发生自剪切后可将目的核酶正确地释放出来 ,计算机设计的单一核酶体外可剪切靶 RNA; 乙型肝炎病毒蛋白对核酶剪切的无明显的抑制或增强作用 ,进一步的结果未观察到乙型肝炎病毒蛋白和靶 RNA的相互作用 .
    To acquire the transcription vector of ribozyme in vitro. Methods The TIMP-1mRNA was taken as the target RNA. Ribozymes were designed according to the "hammerhead structure" described by Symons.
    方法根据 Symons的"锤头结构 ",以人TIMP-1的mRNA为靶 RNA,用计算机对其可能成为核酶切割位点的部位进行分析以寻找最佳切点;
    BACKGROUND &OBJECTIVE: Human papillomavirus is related to cervical cancer. Ribozyme is special kind of RNA that can cleave target RNA.
    背景与目的:人乳头瘤病毒与宫颈癌的发生相关,核酶是一种能切割靶RNA的特殊RNA。
    Ribozyme is a spec ial kind of trans-acting RNA with endonuclease activity and sequence-specific catalytic RNA molecules, which can cleave target RNA.
    核酶是具有特殊核酸内切酶活性的反义RNA,可序列特异性地与靶RNA分子配对并切割靶基因RNA。
    Ribozyme and target RNA were incubated for 90 min at 37℃ in a reaction buffer to perform the cleavage reaction.
    设计合成针对小鼠Caspase-12 mRNA的核酶,通过PCR方法扩增核酶的转录模板,采用非同位素标记法行体外转录,核酶与靶RNA进行体外切割实验。
 

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