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牙胚
    Smad1,5 expression and modulation in inner enamel epithelia of human teeth germs cultured in vitro treated by fluoride
    氟化物作用下体外培养人牙胚内釉上皮细胞中Smad1、5表达的变化
    METHODS:Human teeth germs were cultured in different densities (10 mg/L and 25 mg/L) of fluoride in vitro with Trowell′s method. To observe the result by immunohistochemical staining.
    方法 :Trowell法体外培养人牙胚 ,用 10mg/L和2 5mg/L的氟分别刺激培养的牙胚 ,免疫组化观察结果。
    Methods Human teeth germs were cultured in different densities (10mg/L and 25mg/L) of fluoride in vitro with Trowell's method. The result was observed by immunohistochemical staining.
    方法Trowell法体外培养人牙胚,分别用10mg/L和25mg/L的氟化物刺激培养的牙胚,免疫组化观察结果。
    Conclusion Fluoride may inhibit the expression of Smad1, 5 in inner enamel epithelia of human teeth germs cultured in vitro.
    结论氟化物能抑制体外培养的人牙胚内釉上皮细胞中Smad1、5表达。
    Effects of acute fluoride intoxication during pregnant on the teeth germs of fetus mice.
    孕鼠急性氟中毒对胚胎牙胚发育的影响
    In situ hybridization methodology study with teeth germs
    牙胚组织原位杂交方法学探讨
    Objective To investigate Smad1,5 expression and modulation in inner enamel epithelia of human teeth germs cultured in vitro treated by different densities of fluoride and to explore the probable mechanisms inside the cells of dental fluorosis.
    目的观察不同浓度的氟化物对体外培养的人牙胚内釉上皮细胞中Smad1、5表达的影响,从细胞内信号转导水平观察氟对骨形成蛋白(BMP)信号转导的影响。
    Objective To discuss the best method for in situ hybridization with teeth germs.
    目的探讨牙胚组织原位杂交的最佳方法。
    Methods Oligonucleotide probes labeled with digoxigenin were used in in situ hybridization to inspect DSPP mRNA expression in various teeth germs.
    方法用牙本质涎磷蛋白DSPP特异性寡核苷酸探针,检测不同时期牙胚DSPPmRNA的表达。
    Conclusion In situ hybridization method established with teeth germs showed sensitivity and specificity.
    结论所用的牙胚组织原位杂交方法敏感性高,特异性高。
 

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