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    After cleavage and sequencing, the S1 gene fragment was connected to pcDNA3.1(+) in the correct orientation. Recombinant plasmids was prepared and transinfected into Hela cells.
    结果:扩增的SARS-CoVS1基因片段801bp亚克隆至pcDNA3.1(+)/S1后,免疫细胞化学试验与Western-Blotting结果表明pcDNA3.1(+)/S1可以在HeLa细胞中表达大小约为32KD的S1蛋白。
    After cleavage and sequencing, the NspA gene was subcloned to pcDNA3.1(+) in the correct orientation. Then the constructed plasmid was transfected into RAW264.7 and COS-7 cells by liposome-mediated gene transfer method.
    结果:成功构建了pcDNA3.1(+)/NspA真核表达载体,以脂质体转染RAW264.7和COS-7细胞后,用RT-PCR检测NspA mRNA的水平,免疫细胞化学法检测蛋白表达,表明重组质粒能在真核细胞有效转录和表达NspA。
    TNFR1 gene was subcloned into pEGFP-N2 vector,and then restriction endonucleases assays showed the correct orientation.
    将pMD-TNFR1中TNFR1基因亚克隆至pEGFP-N2载体,酶切鉴定无误;
    Adhering to the Correct Orientation Pressing Close to the Teachers and Students and Improving the School Newspaper's Propaganda Ability
    把握导向贴近师生──校报提高宣传水平之管见
    Probe into the Correct Orientation in the Journals of Higher Vocational and Technical Colleges
    试论高职院校学报的定位
    Sticking to the Correct Orientation Deepening the Reform Continuously-On the Ideological and Moral Education in Chinese Teaching Materials in Secondary Schools
    坚持正确方向 不断深化改革——谈中学语文教材的思想道德教育
    We recommend peaceful rise as our choice for contemporary Chinese global strategy on the base of the correct orientation of the position of Chinese global sustainable development.
    在正确定位中国的全球可持续发展位的基础上,我们提出当代中国的全球战略选择是和平崛起。
    The macroscopiclevel means that the goal of the reform of the state-owned enterprise isto offer the correct orientation to the state wned enterprise in order toadj ust the organization of the state-owned enterprise strategically fromthe function of the state-owned enterprise in order to satisfied thedemands of socialist market economy.
    从宏观层次即是国企改革的目标应该按照社会主义市场经济的要求,从国有企业的功能出发,给国有企业以正确的定位,对国有经济的布局进行战略性调整。
    The fourth chapter brings forth a model of Clustering Analysis to illustrate the ports group of the Yangtze Delta, from which the author draws conclusion about the correct orientation and assignment of ports in the ports group, hence further establishes Shanghai's status as an international shipping center.
    第四章利用聚类分析模型对长江三角洲地区的港口群进行聚类分析,利用模型的结论得出了港口群各个港口的正确定位与分工,进一步确立了上海的国际航运中心的重要地位。
    finger out the correct orientation and road the intellectual should be on;
    指明了当代知识分子成长的正确方向和道路;
    The recombinant vector, pFast-P450nor, was constructed by inserting the cytochrome P450nor gene from Cylindrocarpon tonkinense into the BamH I/Xba I sites of the transposing vector pFastBacl in the correct orientation with respect to the polyhedrin promoter. After transformation, pFast-P450nor was introduced into the competent cells (E.coli DHlOBac) containing a shuttle vector, Bacmid.
    再将其转化进入含穿梭载体Bacmid的受体菌DH10Bac中发生转座作用,得到含P450nor基因的重组穿梭载体rBacmid pAc-P450nor。
    Transport Planning is one of the government important instructions for the development of the transport industry. The scientific nature of the Transport Planning defines the correct orientation of the transport industry and is the prerequisite and foundation for its surmounting development.
    交通规划是政府指导交通行业发展的重要依据之一,交通规划的科学性决定了交通行业发展的正确取向,是交通行业跨越性发展的前提和基础。
    The forth chapter draws conclusion about the correct orientation and assignment of port in the ports group.
    太仓港、嘉兴港、台州港、南通港四港要逐步形成协同发展的内支线港和喂给港。
    improve thetransparency of government's information, and ensure that the correct orientation is maintained in public opinion;
    提高政府信息的透明度,加强舆论监督;
    Therefore, how to take the correct orientation of China papermaking industry in the worldwide scope and how to increase the international competitiveness of China papermaking industry is of crucially strategic importance and realistic emergency.
    波特的“五因素钻石模型”理论,通过:生产因素、需求条件、支持与相关产业、企业的战略、结构与竞争状况、政府等五大要素的对比分析,来说明中国造纸化学品产业的国际竞争力水平,得出的结论是我国造纸化学品行业是一个发展空间较大的产业,但综合产业竞争力还很薄弱。
    In order to provide theoretic and positive support to the correct orientation of company' s marketing strategy for the next phase, bases on marketing strategy theories on environment analysis, strategy selection, plan supporting and other series of academic foundation, this thesis makes a positive research on the marketing strategy of ND Corporate, a corporate in furious market reform of communication industry.
    其结合战略理论中关于营销方面环境分析、战略选择、策略支撑等一系列理论基础对身处通信行业剧烈市场变革中的ND公司市场营销战略进行实证研究,以期对该公司下一阶段市场营销战略的准确定位提供理论和实证支持。
    In this pa-per, we described the correct orientation coordinate system for LiNbO_3 and LiTaO_3. Thedetailed standard pole figures marked with indices of the poles of crystallographic direc-tions and the typical X-ray Laue patterns and the data of diffraction angles of various cuttype plates for the precise determination of orientation were also given.
    本文根据国际上通用的三方晶系转换为六方晶系时的标准正取向坐标系,给出了供晶体劳埃定向用的标有伪对称坐标系及劳埃斑点强度的LiNbO_3和LiTaO_3的详细标准晶面极图及晶向极图。 给出了典型的X射线背射劳埃图谱和不同切型晶片的方位及精密取向测定用的衍射数据。
    The orientation of reformation and achievements for the last three years were affirmed, and it was put forward as well that all practicality should be attached importance to in deepening reformation in order to keep to the correct orientation, To win the victory of the reformation, the principle of materials and interests should be implemented and regulating economic relationship should be regarded as motive force of reformation.
    既肯定了改革的方向及三年来取得的成就,又提出了深化改革应重视多种实践性的研究,才能把握住改革的正确方向; 而争取改革的成功,又必须贯彻物质利益原则,把调节好经济关系做为推动改革的动力。
    coli JM103. When placed downstream from a hybrid trp-lac promoter of vector pKK233-2, in the correct orientation, the cDNA encoding the human clotting factor Ⅸ programs the synthesis of the factor-like-protein, as judged by SDS-PAGE and by ELISA.
    实验结果,我们得到重组表达质粒pKK233-FIX1和pKK233-FIX2,以IPTG诱导,在大肠杆菌JM103中表达出一特定的、与人凝血因子Ⅸ基因cDNA表达相关的蛋白质。 ELISA定性实验表明:这一特定的蛋白质具有人凝血因子Ⅸ抗原性。
    Without a dear understanding of the essential characteristics of “socialization of production” and its ob- jective demands, it is impossible to point out the correct orientation of socialist ownership reform.
    不摘清“生产社会化”的本质特征、客观要求,就不可能对社会主义所有制改革指出正确的发展方向。
 

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