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    ObjectiveTo explore the method of co-culture of Schwann cell(SCs) with fascia and provide experimental basis for repairing transected nerve.
    目的探讨Schwann细胞与筋膜共同培养的方法 ,为神经缺损修复提供实验基础。
    Aim: We have studied the degeneration and regeneration of the Meissner corpuscles in order to estimate the clinical value of the delayed repair of peripheral nerve injuries. Methods: The specimens were taken in 8, 12, 30, 50 weeks after transections of the digital nerves respectively. Also we repaired the transected nerves 1 and 30 weeks after transection and took the specimens 50 weeks after repairment to observe the regeneration of Meissner corpuscles.
    目的:通过观察Meisner小体的退变与再生过程,以评价修复晚期周围神经损伤恢复感觉功能的临床价值.方法:将恒河猴的示,中,环,小指随机分成四组,切断指神经后第8,12,30,50周光镜观察Meissner小体退变过程;
    Making a muscular bridge between the transected nerve end and treating the proximal segment of the injured nerve with low power He Ne laser can significantly promote regeneration and functionnal reconstruction of the injured nerve.
    结果显示,在术后2周内神经干断端的近侧段用小剂量氦氖激光照射,有利于离断神经的再生和修复。
    In group A, a segment of the transected nerve was transplanted into the sciatic nerve of the same rat after freezing and thawing.
    A组为冷冻神经移植于自体原位中; B组为冷冻神经移植于异体中;
    In group B, a segment of the transected nerve treated with the same procedure was transplanted into the sciatic nerve of another rat. The grafts without cryotreatment in group C,D were transplanted in the same manner with A,B respectively.
    C组为未冷冻神经移植于异体中; D组为未冷冻神经移植于自体原位中。
    Results:Different from previous report,an enzymical reaction within 10 mm of the proximal end of the transected nerve was found in one week after injury,and the morphological change of axons could be found 6 hours after injury.
    结果 :与以往组织学观察所见不同的是 ,神经损伤平面近端 10mm范围内 ,1周内存在酶组织化学损伤性反应 ,6h起出现轴索的形态学改变。
    Conclusion:Phase of nerve degeneration and change in the proximal end of transected nerve could be found earlier by immunohistochemical study after injury. S 100 may be an important marker for normal or activated Schwann′s cell.
    结论 :采用免疫组织化学的方法能够更早的观察到神经变性的发生 ,神经损伤近端观察到更广泛的变化 ,S -10 0蛋白可能是标示雪旺氏细胞活化或正常雪旺氏细胞的重要观察指标
 

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