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    Primers were designed according to that, then two deletion fragments JP58B(1093 bp) and JP58S(462 bp) were obtained by PCR using Oryza sativa (japonica cultivar-group) etiolated seedling total DNA as template. The sequencing result showed that they possessed conservative elements of most higher plants promoter.
    在此基础上设计引物,以粳稻日本晴Oryza sativa (japonica cultivar-group)黄化苗总DNA为模板采用DNA聚合酶链式反应(PCR)的方法扩增出基因上游1093bp和462bp两个不同长度的启动子缺失片段(分别命名为JP58B和JP58S),测序结果显示其具有大多数高等植物启动子的保守元件。
    Polymorphism of the ACE gene was categorized into three genotypes: two deletion alleles (genotype DD), heterozygous alleles (genotype ID), and two insertion alleles (genotype II): Serum ACE levels were 28.34±9.88U/ml, 24.82±8.39U/ml, 19.18±4.8U/ml for DD, ID, II respectively.
    血清ACE水平在DD、DI、I型分别为28.3±9.9U/ml、24.5±8.4U/ml、19.2±4.8U/ml,即DD>DI>ⅠⅠ。
    The result showed that the I/D polymorphism in intron 16 of the ACE gene was categorized into three genotypes: two deletion alleles (genotype DD),heterozygous alleles (genotype ID),and two insertion alleles (genotype II).
    结果位于ACE基因内含子 16的I/D多态性经PCR扩增后呈三种基因型 :纯合子插入型 (II)、纯合子缺失型(DD)和杂合子插入或缺失型 (I/D)。
    Conclusion: There were two deletion sites in Fcgr2 b gene promoter region in NZB mice and the deletions could induce hypergammaglob ulinemia.
    结论 :NZB小鼠Fcgr2b基因启动子区存在碱基缺失 ,且该缺失突变可能与血清总IgG水平升高有关
    Conclusion:There were two deletion sites in Fcgr2b gene promoter region in NZB mice and the deletions could induce decreasing of the expression of Fcgr2b mRNA that lead to hyper-IgG.
    结论:NZB小鼠Fcgr2b基因启动子区存在碱基缺失,且该缺失突变可引起其表达的降低而导致高IgG血症
    Primers were designed, and then two deletion fragments, JP58B (1 093 bp) and JP58S (462 bp), were obtained by PCR using total DNA of O. sativa etiolated seedling as template. The sequencing result showed that they possessed conservative elements of most higher plants′ promoters.
    在此基础上设计引物,以粳稻Oryzasativa(japonicacultivar-group)日本晴黄化苗总DNA为模板,采用DNA聚合酶链式反应(PCR)的方法扩增出基因上游1093bp和462bp两个不同长度的启动子缺失片段(分别命名为JP58B和JP58S),测序结果显示,其具有大多数高等植物启动子的保守元件.
    Wild type and two deletion mutants of MP gene were cloned into pEmu-mcs-N and constructed the plant expression vector, then transformed into wheat cultivar Yangmai 158 by biolistic particles.
    分别把运动蛋白(MP)全长基回片段、缺失 N端门个氨基酸的基因片段(So)。 缺夫C端19个氨基酸的基因片段臼Q)和缺失两端36个氨基酸的基回片段门3Q入克隆到表达质粕pEmu-mesN上,构建植物表达质粒pEmuMP、pEmu5Q、pEmu3Q和 pEmu臼Q。
    So we carried out deletion to the three corresponding genes of wild type strain 8004, which are Xcc-1^ Xcc-224 and Xcc-3387. The results revealed that the deletion of Xcc-114 leaded to the deficiency in EPS production, and the other two deletion mutants still produce EPS. So we can conclude that Xcc-114 is a gene involved in EPS production.
    为了验证这3个ORF与胞外多糖的产生有关,我们成功地构建了这3个ORF的缺失突变体。 结果表明Xcc_114缺失突变株的表型与相应ORF的Tn5插入突变株的表型一致,表现为胞外多糖缺陷,而另外两个缺失突变株与野生型8004一致,未表现出胞外多糖缺陷。
    (2) The I/D polymorphism in intron 16 of the ACE gene was categorized into three genotypes: two deletion alleles (genotype DD), heterozygous alleles (genotype ID) and two insertion alleles (genotype II). There was no significant different in genotype pattern of DD, ID.
    (2) 位于ACE基因第16内含子的 I/D多态性经PCR技术扩增后分为三种基因型:纯合子缺失型(DD),纯合子插入型(Ⅱ)及杂合子插入/缺失型(ID)。
    In order to identify the key functional regions of the promoter, the authors insert R8PN's two deletion sequences into another vector pBI121 to take place of CaMV 35S promoter to get two expression vectors pRGUS1 and pRGUS2. Re-combinant plasmids were transformed into Agrobacterium tumefaciens EH 105,and the later were used to infect leaves and mature pollen of tobacco.
    四川大学硕士学位论文将二条长度不同的启动子5’端侧翼区缺失体(分别长471bP,260bP)定向插入载体pBll21中,取代原有的CaMV 355启动子,构建了驱动报告基因GUS的植物表达载体pRGUS了,pRG乙召2,通过农杆菌介导的瞬时表达法转化烟草叶片和花粉,快速鉴定启动子片段中起关键作用的区域。
    \ [WT5 HZ]Results\ The polymorphism of the ACE gene was categorized into three genotypes:two deletion alleles(genotype DD),heterozygous alleles(genotype ID),and two insertion alleles(genotype II). \ The frequencies of DD genotype and D alleles in ischemic stroke patients(including hypertensive patients) were significantly higher than that in hypertensives and control subjects.
    结果脑缺血伴发高血压组DD基因型频率(0.44)及D等位基因型频率(0.55)显著高于高血压组(0.22和0.43)及正常人组(0.15和0.37),脑缺血组DD基因型频率(0.39)和D等位基因型频率(0.54)显著高于高血压组及正常人组。
    Results The insertion/deletion poly morphism inintron 16 ofthe ACEgene was determined by PCRand categorized into three genotypes:two deletion alleles (genotype DD) , heterozygous alleles (genotype ID) ,andtwoinsertion alleles (genotype Ⅱ) .
    结果 位于ACE基因第16 内含子的I/D多态性经PCR技术扩增后可分为三种基因型:纯合子缺失型(DD) ,纯合子插入型( Ⅱ),杂合子插入/ 缺失型(ID)。
    The serum ACE concentration was measured by high performance liquid chromatography with the use of an artificial substrate. Results The I/D polymorphism in intron 16 of the ACE gene was categorized into three genotypes: two deletion alleles (genotype DD), heterozygous alleles (genotype ID), and two insertion alleles (genotype II).
    结果 位于 ACE基因第 16内含子的 I/ D多态性经 PCR扩增后呈三种基因型 :纯合子插入型 (II)、纯合子缺失型 (DD)和杂合子插入 /缺失型 (I/ D)。
 

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