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病毒壳抗原
    Significance of anti EB viral capsid antigen antibodies (VCA-IgM/IgG) for serodiagnosis of infectious mononucleosis
    抗EB病毒壳抗原抗体(VCA-IgM、IgG)测定对传染性单核细胞增多症诊断意义的探讨
    Purification and application of viral capsid antigen of Epstein Barr virus
    Epstein-Barr病毒壳抗原的纯化及其在检查血清抗体中的应用
    Several monoclonal antibodies(McAbs) to Epstein—Barrvirus(EB) antigen—Viral capsid antigen (VGA), membrane antigen(MA), early antigen(EA), early antigen—diffuse (EA—D), early antigen—restricted (EA—R), EB unclear antigen (EBNA), EBNA—2 and latent membrane protein (LMP) were tested using a 3 steps amplified indirect immunoperoxidase method on 5 EB cell lines.
    5种EB病毒细胞系通过三步放大间接免疫过氧化物酶方法,检测抗EB病毒抗原的单克隆抗体(McAb)—病毒壳抗原(VCA)、膜抗原(MA),早期抗原(EA)、早期弥漫性抗原(EA—D)、早期限制性抗原(EA—R)、核抗原(EBNA)、核抗原第2型(EBNA—2)及潜在性膜蛋白(LMP)。
    Expression of the Epstein-Barr Virus P150 Viral Capsid Antigen in Escherichia Coli for the Use as Antigen in Diagnostic Tests
    EB病毒壳抗原在大肠杆菌中的表达及纯化
    INHIBITORY EFFECT OF SOME MEDICINES ON THE EXPRESSION OF VIRAL CAPSID ANTIGEN OF EPSTEIN-BARR VIRUS IN B95-8 CELLS
    几种药物抑制EB病毒壳抗原在体外细胞中表达的作用
    EXPRESSION OF EPSTEIN-BARR VIRAL CAPSID ANTIGEN IN INSECT CELLS AND DETECTION OF ANTIBODIES ID THE ANTIGEN
    在昆虫细胞中表达EB病毒壳抗原及其在诊断中的应用
    The expression of Epstein-Barr virus-associated viral capsid antigen(VCA) in E.coli and application in diagnosis
    EB病毒壳抗原在大肠杆菌中的表达与应用
    INHIBITORY EFFECT OF THE ROOT OF MEMBRANOUS MILE VETCH ON THE EXPRESSION OF VIRAL CAPSID ANTIGEN OF EPSTEIN-BARR VIRUS IN B_(95-8) CELLS
    黄芪抑制EB病毒壳抗原在体外细胞中表达的作用
    Study of the Inhibiting Effect on the Expression of Viral Capsid Antigen of Epstein-Barr Virus in B95-8 Cell by Chinese Medicinal Herbs
    中草药抑制EB病毒壳抗原在体外细胞中表达的实验研究
    The viral capsid antigen of Epstein Barr virus was purified from sf9 cells infected with recombinant Baculoviruses and the purified protein was used to establish ELISA and immunno strip method for detection of VCA/IgG and VCA/IgA antibodies in human sera.
    为了建立纯化Epstein-Barr(EB)病毒壳抗原(EBV-VCA)的方法,用于酶联免疫吸附试验(ELISA)检测人血清中的相关抗体,我们用重组昆虫病毒在感染的sf9细胞中表达EBV-VCA。 感染的细胞经裂解后,层析纯化表达产物。
    Aim:To obtain sufficient Epstein-Barr viral capsid antigen and apply it to the diagnosis of Nasopharyngeal Carcinoma based on ELISA.
    获得足够的EB病毒壳抗原,建立ELISA方法用于鼻咽癌的诊断。
    Methods:Using the recombinant DNA technique,a fragment of gene BALF_4 was cloned into vector plasmid pUR291 and a fusion protein consisting of β-galactosidase and the major polypeptide of Epstein-Barr viral capsid antigen (VCA)was expressed in E. coli. After determining the specificity of the fusion protein,the product was purified by DEAESepharose Fast Flow ion-exhange column chromatography.
    方法:将 BALF4基因克隆进入 载体pUR291,在大肠杆菌中表达EB病毒壳抗原与β-半乳糖苷酶的融合蛋白,并通过DEAE-Sepharose Fast Flow离子 交换柱层析对融合蛋白进行纯化,将纯化的蛋白用于 ELISA方法检测人血清中的IgG/VCA和 IgA/VCA抗体,用带 有pUR291质粒的宿主菌裂解液吸收待检血清中的抗β-半乳糖苷酶抗体。
 

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