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大规模制备     
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  large scale preparation
     The Large Scale Preparation of Recombinant Human Bone Morphogenetic Protein-4 Mature Peptide
     重组人骨形成蛋白-4的大规模制备
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     The key technology for titanium resources utilization includes large scale preparation of titanium-rich material,the chlorination process for titanium tetrachloride from the titanium-rich materials containing high content of calcium and magnesium and the oxidation process for titania of rutile.
     中国钛资源综合利用的关键技术包括大规模制备富钛料技术、高钙镁富钛料直接氯化制备TiCl4技术及TiCl4氧化制备金红石型钛白粉技术。
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     Results and Conclusion: An expression amount of 6×His FL fusion protein up to 15% of total bacterial protein was obtained. The purity of fusion protein just by one step metal chelating affinity chromatography could reach over 90%. All processes were proved simple and fast, and may give a foundation for the large scale preparation of recombinant FL in future.
     结果和结论 :6×组氨酸 FL融合蛋白的表达量约占菌体蛋白总量的 15% ,用Ni2 + 离子螯合亲和层析纯化表达产物 ,一次过柱的纯度可达 90 %以上 ,操作程序简便省时 ,是日后FL基因工程产品大规模制备的可行途径
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     Primary studies showed that the fusion antigen could be specifically bind to and elute from anti preS1 antibody coupled Sepharose resin, suggesting that large scale preparation of the fusion antigen is feasible with an immunoaffinity resin.
     初步的分析显示 ,融合抗原能特异地和前S1抗体偶联的Sepharose结合 ,并能被顺利地洗脱 ,表明该免疫亲和介质能用于融合抗原的大规模制备
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     This method has advantages of mild reaction conditions, convenient operation, and is adaptable to large scale preparation.
     此法工艺简便,条件温和,适合大规模制备
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  large-scale preparation
     Comparison of large-scale preparation of recombinant human BMP-4 and BMP-2 mature peptide expressed in E.coli
     大肠杆菌表达的重组人BMP-2和hBMP-4成熟肽大规模制备方法的比较
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     Conclusion This simple method with high yield can be used for laboratory research and large-scale preparation.
     结论本法简单、产率较高,可用于实验室研究和大规模制备
短句来源
     A convenient procedure for the large-scale preparation of mitochondrial DNA (mtDNA) from Peking duck liver is described.
     本文报导了一个从北京鸭肝脏大规模制备线粒体DNA(mtDNA)的方便的方法。
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     This method can also satisfy the requirements of large-scale preparation.
     该法可以满足大规模制备的要求。
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     Purification of native PNGase F is a time-consuming, multistep process, which limits its utilization and large-scale preparation greatly.
     提取和纯化N-糖酰胺酶F是一个十分耗费和耗时的工作,因此市场价格十分昂贵,这大大限制了N-糖酰胺酶的广泛利用和N-糖链大规模制备
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  “大规模制备”译为未确定词的双语例句
     In this paper, the design, experiment and measurement results of ~(153)Gd production by irradiated Eu_2O_3 were described.
     本文介绍了由照射Eu_2O_3大规模制备~(153)Gd的设计、实验和测量结果。
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     Large-scale fabrication and magnetic properties of Ni_(80) Fe_(20) nanowire arrays
     大规模制备Ni_(80)Fe_(20)纳米线阵列及其磁学特性研究
短句来源
     Isolated Mn2O3 nanotubes/fibers were prepared at a large scale with liquid-phase catalysis method.
     采用简单的液相催化法实现了完全离散的Mn2O3纳米管/纤维的大规模制备.
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     Conclusions The findings suggested that HRP Ⅱ had been successfully expressed in pEP8c/BL21(DE).
     结论恶性疟原虫HRPⅡ在原核表达系统pET8c/BL21(DE3)中获得成功表达,为基因工程大规模制备生产HRPⅡ抗原奠定基础
短句来源
     Isolated carbon nanotubes with lengths ranging from 200 nm to 600 nm were produced directly with high yield of 15 g/h when alcohol was chosen as carbon source.
     该文以乙醇为碳源,直接大规模制备长度为200~600nm,以单根状态存在的离散碳纳米管,产量为15 g/h。
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  large scale preparation
Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out.
      
Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins.
      
Large scale preparation and characterization of mucopolysaccharase contamination free heparinase
      
From a large scale preparation of Euglena gracilis, strain Z, besides the acetylenic pigments diatoxanthin and diadinoxanthin and the allene neoxanthin, an additional acetylenic xanthophyll has been isolated.
      
Porcine erythrocytes may provide a good source for large scale preparation of ganglioside GD3 which recently was identified as a human melanoma-associated antigen.
      
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  maxiprep
Expression plasmids for transient transfections were all prepared by standard techniques using a QIAGEN Maxiprep kit.
      
One construct, pSDW12, was isolated from a bacterial pellet using a Qiagen maxiprep column and used as template for sequencing.
      
Plasmid preparations were performed using Qiagen maxiprep kits according to their protocol.
      
  large-scale preparation
Large-scale preparation of the Δ10 form of staphylokinase by in vitro processing of recombinant staphylokinase with purified hum
      
Coeliac active peptides from gliadin: large-scale preparation and characterization
      
During large-scale preparation in a fermentor, the bacteria enter the sporulation stage after 5 h culture, whereupon high larvicidal activity is obtained (LC50 48 h on Anopheles stephensi = 3.1 × 10-5).
      
In this report, a scalable automated process for large-scale preparation of plasmid is described.
      
Large-scale preparation of adherent lymphokine-activated killer (A-LAK) cells for adoptive immunotherapy in man
      
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An improved method for the preparation of transfer factor from human leukocytesis herein described.Pooled leukocytes were disintegrated in cold 40% ethanol with ahigh speed waring blender fitted with a perforated stainless steel cylinder.Thehomogenate was spun in a refrigerated centrifuge,and the supernatant fluid obtainedwas then lyophilised.The dry powder was dissolved in a desired volume of pyrogen-freedeionised water and the solution was then passed through an ultrafiltration membrane(impermeable to cytochromec)to...

An improved method for the preparation of transfer factor from human leukocytesis herein described.Pooled leukocytes were disintegrated in cold 40% ethanol with ahigh speed waring blender fitted with a perforated stainless steel cylinder.Thehomogenate was spun in a refrigerated centrifuge,and the supernatant fluid obtainedwas then lyophilised.The dry powder was dissolved in a desired volume of pyrogen-freedeionised water and the solution was then passed through an ultrafiltration membrane(impermeable to cytochromec)to obtain the ultrafiltrable transfer factor(TFu).Thismethod has several advantages over the dialysis procedure:(1)The procedure issimpler and strict experimental conditions can be maintained.(2)Tissue autolysis andcontamination with bacteria or pyrogen can be minimized.(3)After extraction ofTFu from the leukocytes,the residue may be used for the extraction of “immune” RNA.(4)It is suitable for large scale production of TF.Each unit of ultrafiltrable TFu so obtained was equivalent to 0.5 gm or 4.45±1.35×10~8 leukocytes,containing 6.86±0.72 mg of dry substance,183.24±26.0μgof ribose,797.22±197.07μg of polypeptides and 58.45±10.70 ng of cyclic adenosinemonophosphate.E_(250nm)=7.86±0.98,E_(260)/E_(280)=2.62±0.12.On Sephadex G-25 gelfiltration it reproducibly showed a characteristic elution pattern which was quitedifferent from that of TF_D prepared by the conventional method.The possible causesof this and other differences were briefly discussed.Our TF preparation were nontoxic,non-pyrogenic,non-anaphylaotic and non-antigenic.The delayed-type skin hypersensitivity reaction and cell-mediated immunityof patients treated with TFu were usually augmented.

本文介绍一种制备转移因子(TF)的改进方法。白细胞于冷40%乙醇溶液中被打碎,白细胞匀浆冷冻离心,上清液除去乙醇后再通过超滤薄膜,滤液即为TFu 制剂。此法操作简便,条件严格,可避免细胞内成分的自溶及细菌或热原的污染,又可综合抽提白细胞内的“免疫”核糖核酸,适合于大规模制备TF。每单位TFu 相当于0.5克或4.45±1.35×10~8白细胞的超滤物,含固体6.86±0.72毫克,核糖183.24±26.0微克,多肽797.22±197.07微克,环化腺嘌呤核苷酸58.45±10.70毫微克。E_(250毫微米)=7.86±0.98,E_(260)/E_(280)=2.62±0.12。具有特征性葡聚糖G-25凝胶过滤洗脱图谱。动物实验证明此制剂无毒性、热原、过敏性和抗原性。注射于病人能提高其迟发型皮肤变态反应及细胞免疫反应。讨论了由透析法及本法制备的TF 理化性质差别的原因。

The venom of Agkistrodon halys Pallas (from Chekiang)was fractionated on DEAE-Sephadex A-50 into fourteen different fractions. The following enzymatic activities, i. e., Phosphodiesterase, 5'-nucleotidase, proteinase, amino acid esterase, L-amino acid oxidase and phospholipase A, as well as hemorrhagic, anticoagulant and plasmin-like principles have been determined for each fraction. A simple and economic method of the purification of the phosphodiesterase on a large scale is described. The quality of the product...

The venom of Agkistrodon halys Pallas (from Chekiang)was fractionated on DEAE-Sephadex A-50 into fourteen different fractions. The following enzymatic activities, i. e., Phosphodiesterase, 5'-nucleotidase, proteinase, amino acid esterase, L-amino acid oxidase and phospholipase A, as well as hemorrhagic, anticoagulant and plasmin-like principles have been determined for each fraction. A simple and economic method of the purification of the phosphodiesterase on a large scale is described. The quality of the product has been characterized and compared with the same product from other countries. Satisfactory results have been achieved in the analysis of the ribose and deoxyribose oligo-nucleotides by this enzyme.

应用二乙胺基乙基葡聚糖A-50和盐浓度梯度洗脱的方法,对浙江产蝮蛇毒进行了柱层析分离,获得14个蛋白峰,测定了磷酸二酯酶、磷酸单酯酶、5'-核苷酸酶、蛋白水解酶、氨基酸酯酶、L-氨基酸氧化酶、磷脂酶A、出血毒素、抗凝血活酶组份以及血纤溶酶样物质在蛋白质峰中的分布。介绍了一种大规模制备磷酸二酯酶的简便、经济的纯化方法,对用这种方法制备的酶制剂的某些主要质量标准进行了分析鉴定并与国外同类产品进行了比较,该酶用于人工合成核糖和脱氧核糖寡核苷酸的顺序分析获得了较为满意的结果。

A convenient procedure for the large-scale preparation of mitochondrial DNA (mtDNA) from Peking duck liver is described. The crude DNA was extracted from mitochondria with 0.8% SDS, then deproteinized with 1M NaClO4 and chloroform, and the macromolecular RNAs were removed with 2M NaCl. Finally the mtDNA was purified by gel filtration on a column of Sepharose 4B. The properties of the mtDNA were identified by uv absorption spectrum, gel electrophoresis, chemical analysis and electron microscopy. The mtDNA of...

A convenient procedure for the large-scale preparation of mitochondrial DNA (mtDNA) from Peking duck liver is described. The crude DNA was extracted from mitochondria with 0.8% SDS, then deproteinized with 1M NaClO4 and chloroform, and the macromolecular RNAs were removed with 2M NaCl. Finally the mtDNA was purified by gel filtration on a column of Sepharose 4B. The properties of the mtDNA were identified by uv absorption spectrum, gel electrophoresis, chemical analysis and electron microscopy. The mtDNA of duck liver consists of circular DNA molecules. The contour length of 63 opea circular mtDNA molecules was measured by electron microscopy and the mean value is 5.2±0.20μm. The mean molecular weight of 37 mtDNA molecules was measured to be 16,660×350 base pairs or (10.7±0.22) × 105 daltons using the pBR322 DXA as internal standard.

本文报导了一个从北京鸭肝脏大规模制备线粒体DNA(mtDNA)的方便的方法。从线粒体用0.8%SDS提取粗制的DNA后,用1M NaClO_4、氯仿脱蛋白,用2M NaCl除去大分子RNA,最后用Sepharose 4B柱凝胶过滤纯化mtDNA。对制得的产品用紫外吸收光谱、凝胶电泳、化学分析和电镜等方法进行了鉴定。鸭肝mtDNA为环形分子。对63个开环型mtDNA分子用电镜测量了长度,其平均周长为5.2±0.20微米。用质粒pBR322 DNA作为内部标准,测得37个mtDNA分子的平均分子量为16,660±350碱基对或(10.7±0.22)×10~6道尔顿。

 
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