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大规模制备
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  large scale preparation
    A new method for large scale preparation of trichokirin (TCK),a single chain ribosome inactivating protein from the seed of Trichosanthes kirilowii was established.
    建立了从栝楼种籽大规模制备核糖体失活蛋白(TCK)的方法。
短句来源
    Results and Conclusion: An expression amount of 6×His FL fusion protein up to 15% of total bacterial protein was obtained. The purity of fusion protein just by one step metal chelating affinity chromatography could reach over 90%. All processes were proved simple and fast, and may give a foundation for the large scale preparation of recombinant FL in future.
    结果和结论 :6×组氨酸 FL融合蛋白的表达量约占菌体蛋白总量的 15% ,用Ni2 + 离子螯合亲和层析纯化表达产物 ,一次过柱的纯度可达 90 %以上 ,操作程序简便省时 ,是日后FL基因工程产品大规模制备的可行途径
短句来源
    CONCLUSION Compared with the past method, this method with high yield and activity can be used for laboratory research and large scale preparation.
    结论 本法与以往纯化 C2的方法相比 ,简便可行 ,产率及活性回收率较高 ,适用于实验室研究和大规模制备
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  “大规模制备”译为未确定词的双语例句
    pUDK-HGF has been submitted to Chinese SFDA for clinic trails.
    一、质粒pUDK-HGF的大规模制备和质量分析
短句来源
    MBL is synthesized by liver cell and secreted into the blood, but the concentration of MBL in blood plasma is very low (~1 mg/L), which makes preparing MBL in large-scale impossible and the scientific research using MBL expensive.
    MBL由肝细胞合成并分泌入血,但人血浆中MBL含量极低(~1mg/L),这使得科研和临床应用MBL不但花费高昂而且不可能大规模制备
短句来源
    Conclusion The above findings suggested that tdh ge ne had been successfully expressed i n Trc99A /JM109and paid the preliminary foundation for preparing anti -TDH poly -and /or monoclonal antibod ies used to the diagnosis,con-structing the vaccine candidate and elucidating pathogenesity of Vibrio parahaemolyticus.
    结论tdh基因在原核表达系统Trc99A/JM109中获得成功表达,为基因工程大规模制备TDH抗原,制备抗TDH的多克隆和/或单克隆抗体,构建基因工程菌苗,阐明该菌的致病机制打下了基础。
短句来源
    Conclusion The results suggested that trh gene was successfully expressed in pGEX - 3X/DE3 and laid the preliminary foundation for preparing anti- trh poly-and/or monodonal antibodies used to the diagnosis, constructing the vaccine candidate and elucidating pathogenecity of Vibrio parahaemdyticus .
    结论:trh基因在原核融合表达系统pGEX-3X/DE3中获得成功表达,为基因工程大规模制备高纯度TRH抗原、构建基因工程菌苗、阐明该菌的致病机制奠定了基础。
短句来源
    Conclusion TRH gene had been successfully expressed and pure protein was obtained through pGEX 3X/DE3. This paid the preliminary foundation for preparing anti TRH poly/monoclonal antibodies for diagnosis, constructing the vaccine candidate and elucidating pathogenesity of Vibrio parahaemolyticus.
    结论 trh基因在原核融合表达系统pGEX 3X/DE3中获得成功表达 ,并得到纯度较高的TRH蛋白 ,为基因工程大规模制备高纯度TRH抗原、制备抗TRH的多克隆和 (或 )单克隆抗体、构建基因工程菌苗、阐明该菌的致病机制奠定了基础
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  large scale preparation
Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out.
      
Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins.
      
Large scale preparation and characterization of mucopolysaccharase contamination free heparinase
      
From a large scale preparation of Euglena gracilis, strain Z, besides the acetylenic pigments diatoxanthin and diadinoxanthin and the allene neoxanthin, an additional acetylenic xanthophyll has been isolated.
      
Porcine erythrocytes may provide a good source for large scale preparation of ganglioside GD3 which recently was identified as a human melanoma-associated antigen.
      
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Objective: To prepare the high purified recombinant new type human tumor necrosis factor (nhTNF) with effective method. Methods: nhTNF expressed in E. coli was collected, disrupted and partially purified through ammonium sulfate preipitation. Then the crude sample was further purified by Q Sepharose, S Sepharose ion exchange chromatography sequentially. In SDS polyacrylamine gel electrophoresis the homogeneity of purified TNF was observed. Its specific activity reached 1.1×10 9 U/mg protein. Result:...

Objective: To prepare the high purified recombinant new type human tumor necrosis factor (nhTNF) with effective method. Methods: nhTNF expressed in E. coli was collected, disrupted and partially purified through ammonium sulfate preipitation. Then the crude sample was further purified by Q Sepharose, S Sepharose ion exchange chromatography sequentially. In SDS polyacrylamine gel electrophoresis the homogeneity of purified TNF was observed. Its specific activity reached 1.1×10 9 U/mg protein. Result: The purity of nhTNF was more than 98%, and the recovery rate 79%. Conclusion: A simple and effective purification method for nhTNF is established. It is the basis for large scale preparation and clinical application of the nhTNF.

目的:制备符合临床要求的高纯度新型人肿瘤坏死因子(nhTNF),建立简单、高效的纯化途径.方法:用本室构建的nhTNF基因工程菌,经发酵、收菌、裂菌、硫酸铵盐析,Q-Sepharose及S-Sepharose层析,得到高活性、高纯度的nhTNF单一色谱峰,比活性达1×109U/mg蛋白.结果:nhTNF纯度达98%以上,活性回收率为79%.结论:纯化方法简单,且达到较好效果,为nhTNF的大规模制备和临床应用打下基础.

Recombinant human interleukin 9(rhIL 9) expressed in E.coli was isolated and purified by washing and solubilization of inclusion body,gel filtration chromatography and ion exchange chromatography.The final product displayed a single band with a corresponding purity of 99.2% in SDS PAGE stained by Coomassie brilliant blue R 250,and the total recovery of rhIL 9 was 66%.Molecular weight of rhIL 9 determined by SDS PAGE was 15.5kD.Biological activity analysis showed that rhIL 9 increased CFU Meg formation...

Recombinant human interleukin 9(rhIL 9) expressed in E.coli was isolated and purified by washing and solubilization of inclusion body,gel filtration chromatography and ion exchange chromatography.The final product displayed a single band with a corresponding purity of 99.2% in SDS PAGE stained by Coomassie brilliant blue R 250,and the total recovery of rhIL 9 was 66%.Molecular weight of rhIL 9 determined by SDS PAGE was 15.5kD.Biological activity analysis showed that rhIL 9 increased CFU Meg formation of normal mouse bone marrow cells.This study provided the basis for large scale preparation and further research of rhIL 9.

表达重组人白细胞介素-9(recombinanthumaninterleukin-9rhIL-9)的大肠杆菌经破碎、包涵体洗涤、裂解提取、凝胶过滤和离子交换色谱分离,得到了电泳纯的rhIL-9,回收率66%,分子量与理论值相符,有刺激小鼠骨髓巨核系集落生成的活性.为rhIL-9的大规模制备及更深入的研究奠定了基础

A new method for large scale preparation of trichokirin (TCK),a single chain ribosome inactivating protein from the seed of Trichosanthes kirilowii was established.A comparative study on properties of TCK with trichosanthin (TCS) had been carried out.

建立了从栝楼种籽大规模制备核糖体失活蛋白(TCK)的方法。进行了TCK与天花粉蛋白(TCS)性质比较研究。

 
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