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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
All cell extracts were prepared by sonication with a Branson Sonifier in the cold.
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All antigens were subjected to 1 min of cavitation by use of a Branson Sonifier, model S75, prior to the serological determinations.
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At the end of 2 h, the solution was sonicated for 10 s at 40 watts with a Bransom sonifier microprobe at C.
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Briefly, the cells were sonicated on ice using a Branson sonifier and cell extracts were stored at 70 C.
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Cells were then sonicated for 10 pulses at setting 5, 50% output, with a Branson 450 sonifier.
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Comparison of Extraction Methods by Soxhlet, Sonicator, and Microwave in the Screening of Pesticide Residues from Solid Matrices
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Three types of solvent extraction methods (by soxhlet, sonicator and microwave) for pesticide recoveries in solid matrices were compared and evaluated using the standard addition method.
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aegerita mycelia were fragmented either with a Dangoumeau grinder, an X-press bomb or a sonicator and the efficiency of these three disruption methods were compared.
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A series of control experiments carried out using an ultrasonic cell sonicator probe gave comparable results to those obtained with ESWL.
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The same holds true for whole cells as well as equally prepared enzyme preparations of heterotrophically or autotrophically grown wildtype Chlorella vulgaris, provided the cells are cracked by a "French press" instead of a sonicator (Figs.
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| | The localization of chitinase in the Beauveria bassiana EU-120 is studied by means of ultrasonic oscillation,differential centrifugation of my- celial homogenates and preparation of protoplasts.In order to obtain stable protoplasts the mycelitrm is treated with an incubation mixture containing chitinase,cullulase and as an osmotic stabilize 0.4mol/L NaCl in 0.15mol/L phosphate buffer pH5.5.The activity of chitinase is found essentially in the supernatant from culture solution,and very little in the mycelial... | | 采用酶解球孢白僵菌菌丝细胞壁以获得原生质体,用超声破碎器破碎细胞,并用差速离心等方法,对此丝状病原真菌细胞中几丁质酶的定位进行了研究.采用球孢白僵菌 Eu-120株,分别用几丁质酶、蜗牛酶和纤维素酶,以不同比例混合进行脱壁处理.通过测定培养物上清液及原生质体溶解物的几丁质酶活性,发现此培养物上清液中的酶活性占其总酶活性的81.46%,而原生质体溶解物中酶活性极少.研究表明,球孢白僵菌几丁质酶主要存在于细胞的周质空间,并在菌生长过程中通过细胞壁分泌至细胞外. | | 文摘来源 | |   |
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