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起始培养
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     The results showed that in primary culture on the medium MS+2.00-3.00 mg·L -1 6 BA+0.10 mg·L -1 NAA, percentage of axillary bud differentiation was higher than 63%.
     结果表明 :在 MS+2 .0 0 - 3.0 0mg· L-16 - BA+0 .10 mg· L-1NAA培养基上进行起始培养 ,其腋芽分化率达 6 3%以上 ;
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     The results suggested that the medium of MS + BA0. 1mg/L + NAA0. 1mg/L + 2,4 - D2mg/L had the best induction effects, especially in the bracteal leaf during the primary culture.
     结果表明,在起始培养中以Ms+BA 0.1 mg/L+NAA0.1mg/L+ 2,4-D 2 mg/L的效果最好,最易诱导出愈伤组织,其中苞片的诱导效果优于其它外植体。
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     With intact mature seeds as initial materials, callus was induced at hypocotyls after 3 weeks of incubation on MS medium containing TDZ 0.1 mg/L and NAA 1.0 mg/L.
     以完整的成熟种子为起始培养材料,在添TDZ 0.1 mg/L与NAA 1 mg/L的MS培养基上,暗培养三周后在下胚轴处形成愈伤组织,形成愈伤的平均频率为99%。
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     The optimal conditions for lipase production were: wheat flour as nitrogen source and soybean flour as carbon source in medium, initial pH9.4 - 9.5, culture temperature 24 - 26c℃for 34 h.
     产酶最适条件为:碳源小麦粉,氮源豆饼粉,起始培养pH9.4~9.5,培养温度24~26℃,培养周期32~34h。
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     The optimal pH was 7.4 in the range of pH experimented.
     在试验的pH值范围内,起始培养最适pH值为7.4。
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  相似匹配句对
     SITS OF ROOT BUD INITIATION AND ROOT CULTURE IN VITRO
     根芽的起始部位与根的离体培养
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     On the Cultivation of Esthetic Judgment
     审美能力的培养
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     LONG-TERM CULTURE OF HUMAN BONE MARROWCELLS ON FEEDERLAYER OF CELLSFROM MURINE ORHUMAN CELL LINES
     长期培养起始细胞在不同的基质细胞层上的培养
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     Dialysis culture
     透析培养
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  start culture
Today, 31 species (49strains) of microalgae, three species (nine strains)of rotifers, one cladoceran and one copepod are holdas start culture.
      


The present paper deals with the estab-

本文报道自安徽省和县大包村采集人恶性疟现症病人和带虫者3倒虫血,脱纤维蛋白后,直接进行体外培养。虫体生长良好,到目前为止其中1例培养已达116天,另2例培养达95天,并有配子体出现。实验表明用于起始培养的虫血原虫密度较低仍可培养成功。

This study was performed to observe the erythrocytic schizogony of P. vivax under several culture conditions in vitro. Five experimental groups included: 1) static cultivation in candle jar; 2) static cultivation in candle jar with candle relightened; 3) static cultivation under low oxygen tension (5-10%); 4) deep suspension cultivation in test tubes with cotton plunger; 5) deep suspension cultivation in closed test tubes with screw cap. Two different isolates of P. vivax collected from Jingshan County and Zaoyang...

This study was performed to observe the erythrocytic schizogony of P. vivax under several culture conditions in vitro. Five experimental groups included: 1) static cultivation in candle jar; 2) static cultivation in candle jar with candle relightened; 3) static cultivation under low oxygen tension (5-10%); 4) deep suspension cultivation in test tubes with cotton plunger; 5) deep suspension cultivation in closed test tubes with screw cap. Two different isolates of P. vivax collected from Jingshan County and Zaoyang County were used. Cultivation was initiated with two methods, i.e. direct inoculation from fresh patient blood with malaria parasites and retrieval cultivation from freezing malaria parasite blood. The suspension cultivation in test tube with cotton plunger could not support the schizogony of P. vivax, while other groups could at least complete two schizogony cycles. The best result was obtained with static cultivation under low oxygen tension, the growth of parasites appeared to be more normal. The results showed that cultivation of P. vivax under a low oxygen concentration of 5-10% is preferred and the selection of isolates of P. vivax might be important in in vitro cultivation.

在培养液成分基本不变的情况下,观察几种体外培养条件的间日疟原虫红内裂体增殖.实验组为:烛缸静止培养,重复点烛烛缸静止培养,低氧静止培养,两种试管深层悬浮培养。引种用了两个虫株,起始培养采用冷冻虫血复苏和现症疟疾病人新鲜虫血直接引种。除棉塞试管深层悬浮组不能支持裂体增殖外,其他组均至少不同程度地完成两个裂殖周期;在低氧下,原虫发育更正常。观察发现,间日疟原虫分离株在体外培养中是重要的。

The results of the research on cultivation of test-tube young plants of Morinda Officinalis How are reported in this paper, which provide a new way for rapid cultivation on a large scale and for resistence seedling selection of these plants. The results showed that: 1) The better sterilization method for explants (apical buds, axillary buds, segments of stem and leaflet) was that rinsing the explants with tap water→pretreatment with 70% ethanol for 5 seconds→sterilizing with 0.1% Hgcl_2 solution for 10 to 15...

The results of the research on cultivation of test-tube young plants of Morinda Officinalis How are reported in this paper, which provide a new way for rapid cultivation on a large scale and for resistence seedling selection of these plants. The results showed that: 1) The better sterilization method for explants (apical buds, axillary buds, segments of stem and leaflet) was that rinsing the explants with tap water→pretreatment with 70% ethanol for 5 seconds→sterilizing with 0.1% Hgcl_2 solution for 10 to 15 minutes→rinsing with sterilized distilled water 5 times. 2) The culture efficiency of apical buds or axillary buds was higher than that of segments of stem and leaflet. 3) The best procedure of test-tube plant cultivation was that sterilized apical buds or axillary buds were cultured on the multiplicating agar medium, clustered adventitious buds were differentiated from those explants after 40 days of culture. They were then subcultured on the rooting agar medium, and after 30 days of culture became the test-tube plants. The test-tube plants were cultivated in indoor sand culture for two weeks, and then transplanted to pot culture for about 1-2 months. Afterwards they were transplanted in the open-air for cultivation.

本文报道了对我国著名的南药之一的巴戟天(Morinda officinalis How)试管苗培养的研究结果,为这种名贵药材的优良种苗的快速大量繁殖和抗性良种的选育提供了新途径。结果表明:(1)对巴戟天外植体(顶芽、腋芽、茎段和叶片)的较好的消毒方法是:外植体经自来水洗净→70%乙醇预处理5秒钟→0.1%HgCl_2溶液中消毒10至15分钟→无茵水漂洗5次,其消毒效果为70~75%,对外植体基本无损伤; (2)顶芽和腋芽的培养效果比茎段和叶片的培养效果好,因此应优先择选顶芽和腋芽为巴戟天试管苗的起始培养物; (3)试管苗的最佳培养程序为:经消毒的顶芽或腋芽在增殖培养基(MS+NAA0.5+BA6.0+GA。1.0+3%蔗糖,单位:mg/L.下同。)中培养40天后可分化出丛生的不定芽(分化率为86.7%),将这些不定芽转入生根培养基(1/2MS+IAA0.2+NAA0.4+1%蔗糖)中培养30天即可分化生根形成完整的巴戟天试管苗,其根分化率为84.6%。试管苗经过两周的沙培室内炼苗后即可移栽盆植,约1~2个月后即可移栽至露天土地种植,移栽成活率大干90%。

 
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