Vitrified MII oocytes were fertilized with fresh spermatozoa and only 4.9% eggs cleaved after 48 culture,which was very significantly lower than the cleavage rate of control(49.5%,P <0.01).
The frozenthawed and fresh spermatozoa were used in this experiment from the proven fertility boars. Caffeine and hyaluronic acid were added as capacitation reagent and incubated with spermatozoa in 39.5℃ and 5% CO2 incubator. At different stages of incubation, motility was examined under a light microscope;
103 sows, which had farrowed severeal times, were used in the field trials, and divided into four groups: fresh semen and semen stored for one, two and three days.
The motility of goat and buffalo sperm decreased significantly as the sheath pressure increased from 10spi to 50psi, 69 %, 67%, 56% , 22%, 8% and 73%, 49%, 22%, 6%,3% at 10psi, 20psi, 30psi, 40psi, and 50psi respectively.
The insemination rates were 77.6%, 54.4% and 73.5%, embryo cleavage rates were 92.9%,94.0% and 92.5%, A-grade embryo rates were 65.4%, 45.7% and 46.8% and clinic pregnancy rates were 45.5%,25.0% and 29.3%, respectively.
The pretreatment before freezing of setting different time, different ratio and time of centrifugal, different remnant of liquid on boar novelty semen were made, compared the effect of sperm freezing result on the different pretreatment.
The number of positive cells and the intensity of the fluorescence were determined by flow cytometric analysis comparing fresh spermatozoa with cryopreserved ones.
Conclusions: Mouse spermatozoa cool-preserved in EFsolution possesses as much fertilizing capacity as fresh spermatozoa.However, prolonged preservation affects theembryonic development.
There was no difference in the proportion of fertilized ova or degenerated embryos resulting from the control or trypsin-treated samples of frozen-thawed semen, which is consistent with results obtained previously using fresh semen.
Fresh semen and blood samples from 20 Karan Fries bulls (4-6 years of age), maintained at the Artificial Breeding Complex of the National Dairy Research Institute, Karnal, were collected for one year.
The previous experiments conducted in 1962 on the storage of boar semen showed, that the sperm well survived in the milk-citrate-glucose-yolk medium at 13-18℃. This study presents the fertility results of sows inseminated with semen after various intervals of storage in this medium. 103 sows, which had farrowed severeal times, were used in the field trials, and divided into four groups: fresh semen and semen stored for one, two and three days. Sows were inseminated two times with interval of 8-10 hrs. durin...
Semen samples were taken from 110 normal fertile Chinese. After they were washed threetimes, sperm (15×10~6/ml) agglutination caused by lectins (WGA, SBA and ConA, 30μg/mlrespectively) was measured by spectrophotometric analysis (wavelength 500 nm) andobserved using SEM as well. The optical density difference between T 20 and To ofthe experimental groups was significantly different from tho control value. (p<0.01) It issuggested that the optical density difference of the lectin treated groups may serve as a...
15 samples of fresh semen from normal adults were used in this experiment and divided into two parts. One aliquot was incubated with Gossypol Acetate(25 ug/ml) and another aliquot was incubated with PBS as control. After 1 hour incubation sperms from both groups were investigated by electron microscopy (SEM and TEM) Wheat Germ Agglutinin (WGA) receptors on sperm membrane from both groups were also measured by spectrophotometry. The results showed that under the direct effect of Gossypol severe ultrastructur...