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模板转换
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  template conversion
     Implementation of Template Conversion Technology in Networ k MIB
     网际管理信息库中模板转换技术的实现
短句来源
     fSAP makes control granularity of artifact in software production reduced to element grade of model, which provide the solid foundation for model conversions by template conversion engine and validity of model.
     fSAP柔性软件自动化生产线使得软件生产对于工件的控制粒度将细化到模型的元素级别,为使用模板转换引擎对工件所表述的模型进行转换以及模型的有效性验证提供了坚实的基础。
短句来源
  “模板转换”译为未确定词的双语例句
     Some template examples are used to explain the mapping patterns of State, View, Model and Persistent Model.
     详细分析了模板转换所需要的两个层次的模式,结合了具体模板实例加以说明在State、View、Model、Persistent Model 4个方面的映射模式;
短句来源
     METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly(A) +RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′ oligo as a short extended template was added to the 5′ end of mRNA to enrich the full length cDNAs.
     方法 :运用mRNA 5′末端的模板转换方法 ,即SMART技术 ,以纯化的Poly(A) + RNA为模板 ,用Powerscript逆转录酶进行转录 ,并在mRNA的 5′末端添加一段 5′ oligo作为延伸后的模板 ,从而富集全长cDNA .
短句来源
     Methods By using the template-switching mechanism at 5′end of mRNA technique to construct the library, a powerscript reverse transcriptase was used to transcribe, and a 5′-oligo fragment as an extended template was added to 5′ end of mRNA to enrich full-length cDNAs.
     方法 运用mRNA 5′末端的模板转换方法以powerscript逆转录酶进行转录 ,在mRNA的 5′末端添加一段 5′ oligo做为延伸后的模板 ,从而富集全长cDNAs。
短句来源
     By using switching mechanism at 5′ end of RNA transcript technology,a seedling full-length cDNA library of canola variety Huashuang 3 was constructed.
     利用RNA5′端模板转换技术,以甘蓝型双低油菜华双3号幼苗为材料,构建了其全长cDNA文库。
短句来源
     Sequence analysis showed that a contiguous segment of 2380 nucleotides was internally deleted from the WYMY RNA1 and flanked by direct repeats of six nucleotides CGTCTC, which supports the "copy choice" model as the possible mechanism of the generation of WYMV D-RNA 1.Associated with WYMV, D-RNA 1 appears to increase manual inoculation efficiency, shorten the latent period and exacerbate symptoms displayed in plants, which stabilized gradually in the late passages after the thirteenth (P13).
     据此对D-RNA1的缺失机制进行了讨论,认为由一种模板转换机制导致了缺失的发生。 在连续机械接种导致产生D-RNA1的同时,含有D-RNA1的WYMV能够明显加重发病症状,接种发病时间缩短,接种发病率也有所提高,并逐渐趋于稳定。
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  相似匹配句对
     A design method of yemtlet&bracket of yransfer story floor
     转换层楼板模板及支架设计
短句来源
     Implementation of Template Conversion Technology in Networ k MIB
     网际管理信息库中模板转换技术的实现
短句来源
     The Machining of the Die Plate
     模板的加工
短句来源
     The Formwork of Creation
     创造的“模板
短句来源
     Transforming Bridge
     转换
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  template switching
It was believed until recently that the only possible mechanism of RNA recombination is replicative template switching, with synthesis of a complementary strand starting on one viral RNA molecule and being completed on another.
      
Recombinant mitochondrial DNA molecules suggest a template switching ability for group-II-intron reverse transcriptase
      
The structure of these junctions led us to propose that they were most probably initiated by a RNA template switching of the reverse transcriptase encoded in COX1-i1.
      
The genes are fragmented into randomly sized pieces, and polymerase chain reaction (PCR) reassembly of full-length genes from the fragments, via self-priming, yields recombination due to PCR template switching.
      
This template switching may be involved in the retroelement integration process.
      
更多          
  template switch
The presence in the mammalian genome of not only "double" but also "triple" chimeric retrogenes indicates that not only a single but also a double template switch may occur during L1-catalyzed reverse transcription.
      
It was shown that the shuffling mechanism for transcribed genome components, which involves a template switch during the RNA reverse transcription using the L1 retroelement enzymatic machinery, is common in mammals.
      
Analysis of primer extension and the first template switch during human immunodeficiency virus reverse transcription
      
The template switch effect is applied to incorporate a primer containing both an anchoring primer site and a RNA polymerase binding site.
      
We speculate that a template switch in the A-tail region during the amplification is a possible mechanism for this expansion.
      


This paper was to study the molecular character of HBV preS mutation in chronic hepatitis B patients and chronic HBV carriers and accordingly to discuss its possible mechanismHBV DNA extracted from sera of chronic hepatitis B patients and HBV carriers were subjected to polymerase chain reaction,and then cloning and sequencingThe mutational structural characters were analyzed,the possible mechanisms were put forwardThe PreS gene segment was ...

This paper was to study the molecular character of HBV preS mutation in chronic hepatitis B patients and chronic HBV carriers and accordingly to discuss its possible mechanismHBV DNA extracted from sera of chronic hepatitis B patients and HBV carriers were subjected to polymerase chain reaction,and then cloning and sequencingThe mutational structural characters were analyzed,the possible mechanisms were put forwardThe PreS gene segment was amplified from sera of 262 hepatitis B patients and 103 asymptomatic carriers,different kinds of mutations were found in 30 casesThe deletions were mainly located in 3′end of PreS1 and 5′end of PreS2Among these mutations,inframe 183bp deletion(nt3019nt3201)was found in 9 cases,this mutation coincided with mRNA splicing mechanism in mammalian cell and was highly conserved in all genotypes129bp deletion(nt3019nt3147)and 91bp deletion(nt3019nt3109)were also detected and also coincided with mRNA splicing mechanismTwentythree different kinds of deletions were found among repeat sequences,which coincided with template switch mechanism during reverse transcriptionThe secondary structures of pregenomic RNAs were predicted,only part of deleted sequences correlated to local RNA secondary structureWhen HBV is subjected to mutation under both the external factor,mRNA splicing mechanism,and the internal factor,the function of HBV polymerase,different mutations can be producedBesides mRNA splicing mechanism in mammalian cell,template switch mechanism during reverse transcription is one of the major mechanism for deletion mutation PreS nt3019nt3201, 183bp deletion may be genotypic nonspecific,but its high occurrence may result from selection pressure

检测慢性乙型肝炎病毒(HBV)携带者和患者外周血内HBV前S区基因缺失突变的分子结构特点,探讨其发生机理。用聚合酶链反应方法从慢性乙肝患者和携带者血清中扩增出前S区基因片段,克隆、测序,分析缺失发生的结构特点,从而推测这些前S区基因缺失突变的产生机制。从262例慢性乙肝患者和103例无症状HBV携带者体内扩增出前S区片段,共在30例患者和携带者中检测出多种前S区基因缺失突变,主要集中于前S1区的3′端和前S2区的5′端。其中有9例患者和携带者体内存在完全一样的nt3019~nt3201183bp的缺失突变,该缺失突变符合真核细胞mRNA剪接机制,在此位置上各基因型的序列高度保守。同时有另外两种缺失突变,即nt3019~nt3147129bp缺失、nt3019~nt310991bp缺失也符合该剪接机制。有23种缺失突变部分于重复序列之间,符合逆转录过程中的模板转换机制所导致的缺失。根据前基因组RNA预测出二级结构,仅部分缺失突变在RNA二级结构中对应于局部的结构。此结果表明:HBV在外界因素mRNA的剪接机制和内在因素聚合酶蛋白的功能特点的共同作用下,产生各种突变,不同的机制将导致不同类型的缺失突变...

检测慢性乙型肝炎病毒(HBV)携带者和患者外周血内HBV前S区基因缺失突变的分子结构特点,探讨其发生机理。用聚合酶链反应方法从慢性乙肝患者和携带者血清中扩增出前S区基因片段,克隆、测序,分析缺失发生的结构特点,从而推测这些前S区基因缺失突变的产生机制。从262例慢性乙肝患者和103例无症状HBV携带者体内扩增出前S区片段,共在30例患者和携带者中检测出多种前S区基因缺失突变,主要集中于前S1区的3′端和前S2区的5′端。其中有9例患者和携带者体内存在完全一样的nt3019~nt3201183bp的缺失突变,该缺失突变符合真核细胞mRNA剪接机制,在此位置上各基因型的序列高度保守。同时有另外两种缺失突变,即nt3019~nt3147129bp缺失、nt3019~nt310991bp缺失也符合该剪接机制。有23种缺失突变部分于重复序列之间,符合逆转录过程中的模板转换机制所导致的缺失。根据前基因组RNA预测出二级结构,仅部分缺失突变在RNA二级结构中对应于局部的结构。此结果表明:HBV在外界因素mRNA的剪接机制和内在因素聚合酶蛋白的功能特点的共同作用下,产生各种突变,不同的机制将导致不同类型的缺失突变。除真核细胞mRNA剪接机制外,逆转录过程中的模板转换是主要机制之一。

AIM: To construct the cDNA libraries of renal papillary adenocarcinoma tissue and its side tissue by SMART technology. METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly(A) +RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′ oligo as a short extended template was added to the 5′ end of mRNA to enrich the full length cDNAs. After amplification, the ds cDNA was digested by sfi I,...

AIM: To construct the cDNA libraries of renal papillary adenocarcinoma tissue and its side tissue by SMART technology. METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly(A) +RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′ oligo as a short extended template was added to the 5′ end of mRNA to enrich the full length cDNAs. After amplification, the ds cDNA was digested by sfi I, size fractionated by columns (CHROMA SPIN 400) and then recombined to the λTripIEx 2 vector. After package, the libraries were tittered, the rate of recombination ( blue/white) was determined and then the cDNA libraries were amplified. RESULTS: The titer of the cDNA libraries of renal papillary adenocarcinoma tissue and its side tissue were 1.4×10 6 pfu·mL -1 and 2.6×10 6 pfu·mL -1 , respectively. After amplification, the rates increased to 6×10 11 pfu·mL -1 and 9×10 11 pfu·mL -1 , respectively. CONCLUSION : Our successfully constructed cDNA libraries are full length libraries with high efficiency and can be screened by probes and antibodies to find the associated genes of the renal papillary adenocarcinoma.

目的 :运用SMART技术构建肾乳头状腺癌及癌旁组织的cDNA文库 .方法 :运用mRNA 5′末端的模板转换方法 ,即SMART技术 ,以纯化的Poly(A) + RNA为模板 ,用Powerscript逆转录酶进行转录 ,并在mRNA的 5′末端添加一段 5′ oligo作为延伸后的模板 ,从而富集全长cDNA .cDNA扩增后 ,经sfiI酶切、CHROMASPIN 4 0 0洗脱 ,与λTripIEx2载体连接并用载体蛋白包装后 ,测定滴度和重组率 ,并扩增 ,建成cDNA文库 .结果 :构建的肾乳头状腺癌及癌旁组织cD NA文库滴度分别为 1.4× 10 6pfu·mL-1和 2 .6× 10 6pfu·mL-1,重组率均 >98% ,扩增后滴度分别达 6× 10 11pfu·mL-1和 9×10 11pfu·mL-1.结论 :我们构建的人肾乳头状腺癌及癌旁组织cDNA文库为高效、全长cDNA文库 ,可以用做探针、抗体等免疫学筛选 ,进一步探寻与肾乳头状腺癌相关的基因

Wheat leaves total RNA was reverse transcripted into cDNA using SMART (the Switch Mechanism At the 5'end of RNA Templates) technique. The resulting cDNA was ligated to SfiA and SfiB sites in the vector λTriplEx2. Then the mixture of ligation was packed in vitro and the host strain E.coli XL1 Blue was infected . 1.0056×10 6 recombinants had been obtained. 100 clones were chosen randomly from the library and were screened cDNA inserts using PCR. 81 percent of products were over 500bp. The results showed that...

Wheat leaves total RNA was reverse transcripted into cDNA using SMART (the Switch Mechanism At the 5'end of RNA Templates) technique. The resulting cDNA was ligated to SfiA and SfiB sites in the vector λTriplEx2. Then the mixture of ligation was packed in vitro and the host strain E.coli XL1 Blue was infected . 1.0056×10 6 recombinants had been obtained. 100 clones were chosen randomly from the library and were screened cDNA inserts using PCR. 81 percent of products were over 500bp. The results showed that the cDNA library of wheat leaf had been constructed.

提取小麦辉县红的三叶期幼叶总RNA ,利用RNA5’端模板转换技术 (SMART ,theSwitchMechanismAtthe 5’endofRNATranscript)合成cDNA的第一链 ,再经 2 1个循环的长距离PCR(LD PCR)合成双链cDNA ,经蛋白酶K消化、纯化cDNA、SfiI(A和B)酶切、过CHROMASPIN 4 0 0柱分级分离除去 4 0 0bp以下的小分子 ,与经过 5’端脱磷的λTriplEx2 Sfi(A和B) CIP载体进行连接 ,经体外包装和感染XL1 BLue宿主菌 ,得到了 1.0 0 5 6× 10 6 个独立的噬菌体克隆 .从中随机挑选 10 0个克隆进行PCR反应 ,电泳结果显示PCR产物大于 5 0 0bp的片段占 81% ,由此认为小麦幼叶cDNA文库已建立

 
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