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培养传代
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     All passage cells were separated into 1×109 L-1 cell density, and then were inoculated in L-DMEM culture contained 0.1-volume fraction of fetus ox blood serum (FBS) to go on cultivation and passage.
     传代细胞部分以1×109L-1的细胞密度接种于含体积分数为0.1的胎牛血清的L-DMEM的培养基中继续培养,传代;
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     ④The cultured keratocytes were mixed with type Ⅰ collagen solution at 0.55, 1.1, 1.65, 3.3 g/L mass concentrations to form and culture collagen lattices.
     ④取培养传代的角膜基质细胞,混合于0.55,1.1,1.65,3.3g/L4组质量浓度Ⅰ型胶原溶液中,形成角膜基质凝胶块,在培养皿中培养,观察不同胶原质量浓度对凝胶块收缩的影响。
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     Methods Human lung adenocarcinoma A549 cells were cultured for 72 hours and divided into experimental group A, B, C, D and E based on the concentrations of matrine rang ed from low to high and one control group.
     方法:肺腺癌A549细胞进行培养传代,按苦参碱药物作用浓度由低向高分为A、B、C、D、E组及对照组,培养72h后,MTT实验检测苦参碱对肺癌细胞生长抑制作用;
短句来源
     All passage cells were separated into 1 ~ 106/ml cell density, and then were inoculated in conventional culture that was L-DMEM to be contained 100ml/L fetus ox blood serum (FBS) and went on cultivation and passage.
     传代细胞部分以1×106/ml的细胞密度接种于含100ml/L胎牛血消(FBS)的L-DMEM的培养基中继续培养,传代;
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     0.5×10~(-3)M Dibutyryl c-AMP (db-cAMP) for 3 days .
     用含10~(-8)M AT-RA、0.5×10~(-3)M db-cAMP的培养液诱导3天,然后在普通培养液中培养传代,连续观察21天。
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     Cultivation and passage of chicken primordial germ cells
     鸡胚胎原始生殖细胞的培养传代
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     Cultured cells could stably grew and passage.
     培养的细胞可以稳定生长传代
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     On the Cultivation of Esthetic Judgment
     审美能力的培养
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     Dialysis culture
     透析培养
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  transfer of culture
Documentation is timely not only because there has been great demand for the transfer of culture technology (spawning) to China, but also because the species may become extinct with the demise of the culture business through urbanization.
      
This involves a double filter-paper bridge with a liquid medium for root induction and allows the transfer of culture-grown plantlets to a glasshouse environment with minimal disturbance to the plant as a whole.
      


We succeeded in the establishment of three hybridoma cell lines secreting anti-human IgG monoclonal antibodies by means of the fusion between mouse myeloma cells SP2/O and spleen cells from BALB/c mice immunized with purified human IgG. One of the hybridoma cell lines was named SIBPII1. The McAb secreted by SIBPH1 showed high specific activity with immunodiffusion and hnmunoelectrophoresis techniques. SIBPH1 could produce ascitic fluids with high liter McAb after being inoculated into BALB/c mice I. P. (I. D....

We succeeded in the establishment of three hybridoma cell lines secreting anti-human IgG monoclonal antibodies by means of the fusion between mouse myeloma cells SP2/O and spleen cells from BALB/c mice immunized with purified human IgG. One of the hybridoma cell lines was named SIBPII1. The McAb secreted by SIBPH1 showed high specific activity with immunodiffusion and hnmunoelectrophoresis techniques. SIBPH1 could produce ascitic fluids with high liter McAb after being inoculated into BALB/c mice I. P. (I. D. 1: 256-512). The McAb of SIBPH1 is of mouse IgG1 subclass.This cell line was cloned 4 times successively by limiting dilution method. Karyotype analysis of the subclones showed that the number was 95 ±3.SIBPH1 is stable to secrete anti-human IgG McAb in tissue culture over six months and having been stored liquid nitrogen over twelve months. Some problems of the established cell line were briefly discussed.

本文报道用人IgG免疫BALB/c 小鼠的脾细胞与骨髓瘤细胞系SP2/O融合,获得 3株分泌抗人 IgG单克隆抗体的杂交瘤细胞株,命名为 SIBPH1、SIBPH2和 SIBPH3。现重点报道SIBPH1株。用琼脂双扩散和免疫电泳等方法,证实该株杂交瘤细胞所分泌的单克隆抗体,对IgG具有良好的专一性,注入同系小鼠腹腔可诱生含较高效价抗IgG的腹水(双扩散1:256~512),单克隆抗体属小鼠IgG_1亚类,该杂交瘤细胞株经组织培养传代逾半年,冻存12个月,复苏良好,分泌抗IgG性能稳定。本文还对杂交瘤细胞建株的若干问题进行了讨论。

The N-79 colone-selected LaSota strain of Newcastle Disease virus (abbreviating N-79) can be adapted in the chicken embryo fibroblast (CEF) culture. The CPE appeared and the hemagglutination could be detected at the first and second passages. With the passages increasing, the hemagglutination disappeared, however, the hemoadsorption(HAd)arised obviously. After being inoculated into the chicken embryos which were incubated for 9—11 days, the CEF cultures could lead to death of the embryos. The HA titers of allantoic...

The N-79 colone-selected LaSota strain of Newcastle Disease virus (abbreviating N-79) can be adapted in the chicken embryo fibroblast (CEF) culture. The CPE appeared and the hemagglutination could be detected at the first and second passages. With the passages increasing, the hemagglutination disappeared, however, the hemoadsorption(HAd)arised obviously. After being inoculated into the chicken embryos which were incubated for 9—11 days, the CEF cultures could lead to death of the embryos. The HA titers of allantoic fluid could reach to 1:1280~1:2560.15 passages of the N-79 strain were carried out in CEF. The satisfactory effect of vaccination was obtained after the 18-day-old chickens were vaccinated through 2 times of drinking water (the second vaccinating after a 60 day's interval) by using the passaged cell cultures.

鸡新城疫(N-79型)LaSota毒株(简称N-79)能适应于鸡胚成纤维细胞培养,在第1、2代可引起细胞病变,并能测出血凝现象。随着病毒在鸡胚细胞培养传代次数增加,血凝现象也消失,但出现明显血吸附(HAd)现象。鸡胚细胞培养物接种于9—11日龄鸡胚内,能致死鸡胚,鸡胚尿囊液的血凝价可达1∶1280—1∶2560。 N-79毒株在鸡胚细胞培养连续传15代,对18日龄雏鸡经2次饮水免疫(首免后60天进行再次免疫),获得良好免疫效果。

In this paper, we report our achievements in the estabilishment of one hybridoma cell lines secreting anti-human group A erythrocyte monoelonal antibodies(MC Ab)by means of fusion between mouse myeloma cell NS1 and spleen cells from BALB/C mice immunized withhuman group ARh(+) erythrocyte. The bybridoma cell lines have been named ZMC_9. The result of blood-agglutination test showed that MC Ab secrted by ZMC_9 was of specificity. No Cross reaction was detected in 548 cases of blood sample. ZMC_9 has been proved...

In this paper, we report our achievements in the estabilishment of one hybridoma cell lines secreting anti-human group A erythrocyte monoelonal antibodies(MC Ab)by means of fusion between mouse myeloma cell NS1 and spleen cells from BALB/C mice immunized withhuman group ARh(+) erythrocyte. The bybridoma cell lines have been named ZMC_9. The result of blood-agglutination test showed that MC Ab secrted by ZMC_9 was of specificity. No Cross reaction was detected in 548 cases of blood sample. ZMC_9 has been proved to be in seeretion of MC Ab in tissue culture for 4 months. Inoculation and transplanted ation of ZMC_9 into BALB/C mice could produce ascitic fluids with high titer (1: 262144) and solid tumors.

本文报道了用人Rh(+)红细胞免疫BALB/C小鼠,取其脾细胞与骨體瘤细胞系NS/融合,获得了一株能分泌抗人A型红细胞单克隆抗体的杂交瘤细胞株,定名为ZMC9。血凝试验结果表明,ZMC_9具有较好的特异性。经临床548例血型测定,未见交叉反应。该杂交瘤细胞株经组织培养传代四个月,分泌抗人A型红细胞的性能稳定。注入BALB/C小鼠腹腔产生含高滴度(1∶262.144)的腹水及肿瘤。

 
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