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可变基因
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  “可变基因”译为未确定词的双语例句
     The results indicated that the C1qVL belongs to VK gene family of mouse immunoglobulin,it also showed a high homology with the V_K gene of other species.
     对抗人C1q轻链可变基因(C1qV_L)的分析结果表明:C1qVL属鼠IgVK基因家族,但与其它种属的Ig基因也显示较高同源性。
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     A atypical class 1 integron without 3' conserved region was foundto prevail among Shigella flexneri isolates, and the excision models of it's gene cassette were charactered by inverse PCR.
     典型的Ⅰ类整合子包括有5’保守片段(intI1)、可变基因盒区(引物inF~inB)和3’保守片段(引物qacEΔ1~sul1)。 本文64株福氏和宋内志贺菌株中,三者均阳性浙江大学博士学位论文的菌株仅有6株,阳性率为9.4%。
短句来源
     Methods:The variable regions of the heavy and light chains of platelet specific antibody SZ 2 were amplified by reverse transcription and polymerase chain reaction.
     方法 :应用RT PCR技术 ,克隆SZ 2重链、轻链可变基因
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  相似匹配句对
     4 new genes were obtained.
     U基因
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     The NiCoT gene of S. aureus had been successfully expressed in E.
     外源基因在E.
短句来源
     Antibody variable genes cloning by in cell PCR
     细胞内PCR法克隆抗体可变基因
短句来源
     Current progress on alternative pre - mRNA splicing research in eukaryote genes
     真核基因可变剪接研究现状与展望
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  variable genes
Generation and characterization of an immune combinatorial cDNA library of variable genes of mouse immunoglobulins
      
For this purpose, cDNA of variable genes of heavy (VH) and light (VL) immunoglobulin chains amplified from splenocytes were joined by linker DNA to form single-chain antibodies (single-chain Fv-antibodies, or ScFv's).
      
Sequencing analysis determined that the isolated cells expressed functional immunoglobulin variable genes, consistent with an antitumor antibody response.
      
The human T-cell receptor gamma (TCRG) locus comprises 14 variable genes (TRGV), five joining segments (TRGJ), and two constant region genes (TRGC).
      
Restriction fragment length polymorphisms have been previously documented for the constant region genes, the joining segments, and the variable genes belonging to subgroups I and IV.
      
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omputer-aided sequence analysis plays an importapt role in molecular biology,which is being used increasingly to study the large volume of rapidly accumulating sequence date In the present work,the light chain variable gene of anti-human C1q(C1qV_L)was analysed by this approach.The results indicated that the C1qVL belongs to VK gene family of mouse immunoglobulin,it also showed a high homology with the V_K gene of other species.Among the similar segments,most are relevant to the variable gene of autoantibody...

omputer-aided sequence analysis plays an importapt role in molecular biology,which is being used increasingly to study the large volume of rapidly accumulating sequence date In the present work,the light chain variable gene of anti-human C1q(C1qV_L)was analysed by this approach.The results indicated that the C1qVL belongs to VK gene family of mouse immunoglobulin,it also showed a high homology with the V_K gene of other species.Among the similar segments,most are relevant to the variable gene of autoantibody ,suggesting that C1qVL may be interrelated with anti-C1q autoantibody The information is valuable for the understanding of the effect of ant-C1q antibody in the autoimmune.

运用计算机进行基因序列分析在分子生物学研究中起重要作用,已被越来越多地用于研究大量累积的序列数据,获得许多重要发现。对抗人C1q轻链可变基因(C1qV_L)的分析结果表明:C1qVL属鼠IgVK基因家族,但与其它种属的Ig基因也显示较高同源性。它的另─个显著特点是与自身抗体编码基因高度同源。研究结果对我们进─步认识抗-C1q抗体生理、病理作用具有─定价值。

The sequences of forty one human and mouse TCR Vβ s were aligned, and then HV4 residues in the Vβ s were compared and analyzed. Whether HV4 sequences of four Vβ s(mouse Vβ 3,15,17 and human Vβ 2) binding to superantigen TSST 1 contained any specific motif or not was examined. The results showed that HV4 sequences of mouse Vβ 3 and Vβ 17 contained the specific RFSAXCXSNS motif, while those of mouse Vβ 15 and human Vβ 2 contained unique...

The sequences of forty one human and mouse TCR Vβ s were aligned, and then HV4 residues in the Vβ s were compared and analyzed. Whether HV4 sequences of four Vβ s(mouse Vβ 3,15,17 and human Vβ 2) binding to superantigen TSST 1 contained any specific motif or not was examined. The results showed that HV4 sequences of mouse Vβ 3 and Vβ 17 contained the specific RFSAXCXSNS motif, while those of mouse Vβ 15 and human Vβ 2 contained unique KFXIXH motif. The data indicate that there are possibly more than one superantigenic T cell epitopes in TSST 1 molecules.

首先对41种人和小鼠的T细胞受体β链可变基因编码肽段(Vβ)的氨基酸序列进行多序列对准,就Vβ之第四高变区(HV4)片段进行比较,分析与超抗原毒素休克综合征毒素-1(TSST-1)结合的四种Vβ(小鼠Vβ3、Vβ15、Vβ17和人Vβ2)之HV4序列内是否存在特定的氨基酸残基排列模式。结果发现:小鼠Vβ3和Vβ17的HV4具有特异的RFSAXCXSNS模式,而小鼠Vβ15和人Vβ2的HV4则含独特的KFXIXH模式。提示:与TSST-1结合的四种Vβ所对应的T细胞识别表位可能不止一个。

Objective:To express the recombinant single chain Fv(scFv) in E.coli and reduce immunogenicity and molecular weight of a monoclonal antibody specific for human platelet.Methods:The variable regions of the heavy and light chains of platelet specific antibody SZ 2 were amplified by reverse transcription and polymerase chain reaction.VH and VL gene segments were cloned into pUC Tm and joined together with a (gly 4ser) 3 linker.The resulting scFv was expressed in PET expression system.The expressed recombinant...

Objective:To express the recombinant single chain Fv(scFv) in E.coli and reduce immunogenicity and molecular weight of a monoclonal antibody specific for human platelet.Methods:The variable regions of the heavy and light chains of platelet specific antibody SZ 2 were amplified by reverse transcription and polymerase chain reaction.VH and VL gene segments were cloned into pUC Tm and joined together with a (gly 4ser) 3 linker.The resulting scFv was expressed in PET expression system.The expressed recombinant protein was characterized by its size on SDS PAGE,by Western blot,by flow cytometry and its functions.Results:The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.The recombinant scFv was expressed mostly in the form of inclusion bodies,and the yield was up to 25% of the total cell proteins.Functional studies showed that SZ 2 scFv could bind to platelet and could suppress platelet aggregation induced by ristocatin and thrombin.Conclusion:A recombinant SZ 2 scFv specific against platelet was developed and characterized.

目的 :降低鼠源性抗体的免疫原性及分子量 ,为其进一步研究、应用奠定基础。方法 :应用RT PCR技术 ,克隆SZ 2重链、轻链可变基因。应用基因重组技术构建SZ 2单链抗体表达载体pET2 2 2scFv ,导入大肠杆菌BL2 1(DE3)Plys ,诱导表达。流式细胞术、ELISA、Westernblot检测SZ 2scFv与血小板结合能力 ,瑞斯托霉素、凝血酶和ADP诱导血小板聚集试验对表达产物功能进行研究。结果 :克隆基因序列符合小鼠轻、重链可变区基因特征 ;pET2 2 2scFv表达质粒拼接正确 ;表达产物以包涵体形式为主 ,表达量占菌体总蛋白的 2 5 % ;纯化、复性后证明表达产物具有与血小板结合的活性 ,并可抑制瑞斯托霉素、凝血酶诱导的血小板聚集。结论 :成功表达了SZ 2单链抗体 ,该小分子抗体具有与血小板结合的活性 ,并可抑制瑞斯托霉素、凝血酶诱导的血小板聚集。

 
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