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     Methods McAb clones against C34 and C46 were prepared by hybridoma technique and identified for epitopes by ELISA, for effect on cell proliferation by MTT, and for effect on cell fusion by molecular fluorescence probe.
     方法常规动物免疫、细胞融合、克隆化制备抗C34与C46的单克隆抗体,并鉴定其 特异性位点,还用ELISA法、MTT法及荧光分子探针技术对单抗的生物学活性进行研究。 结果获得了3株抗C34单克隆抗体、2株抗C46单抗。
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     Also such model is valuable in evaluation of effectiveness of vaccines.
     在评价肺结核病疫苗的有效性方面,是许多常规动物模型所不可替代的。
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  相似匹配句对
     Animal;
     动物;
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     Animals
     动物
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     ROUTINE PARAMETER ESTIMATION METHODS FOR ANIMAL GROWTH MODELS
     动物生长模型参数的常规估计法
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     Conventional Nutrient Examination for Some Laboratory Animal's Feed in Heilongjiang Proceinv
     黑龙江省部分实验动物饲料常规养分的检查
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     The Conventional Island
     常规
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  conventional animal
The benzodiazepine/GABA receptor coupled chloride ionophore was examined in brain membranes of rats maintained in either a conventional animal facility or a "protected" (low-stress) environment.
      
To reproduce the complex cellular interactions that occur in human patients is, however, difficult, and has not been achieved using currently available in vitro systems or conventional animal models.
      
Difficulties arise in the maintenance of these wild-derived mice in conventional animal facilities, however, and can be overcome by the use of a congenic strain for the region under study.
      
The predictivity of the in vitro results will be compared with the predictivity of conventional animal tests for the same chemicals.
      
The stress of crowding and exposure to inescapable electric shock increased both the incidence and the severity of dental caries in rats housed in a conventional animal facility.
      
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  conventional animals
In the cartilage (intercellular matrix, lacunar border and chondrocyte cytoplasm) and aorta (interelastic spaces) of germfree animals the reactions were less intense than in those of conventional animals.
      
The ratio of villous height to cryptal depth is distinctly higher in rats reared in germfree conditions than in conventional animals.
      
The reported work is the first direct ultrastructural comparison of resident peritoneal macrophages from germ-free and conventional animals.
      
Similar bacteria were also found when specific pathogen-free rats, lacking mucosa-associated populations, were inoculated with homogenized rat intestine from conventional animals.
      
Feeding the commercial diet reduced the number of argyrophil endocrine cells in the jejunum and serotonin immunoreactive cells in the colon of gern-free animals but increased the serotonin immunoreactive cells in the colon of conventional animals.
      
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The laboratory animal science is a new science. This article reports the relationship between standardization of laboratory animals and life sciences research. Biological background for the standardization of laboratory animals includs genetic background and microbiological background. The genetic background gives five different types: inbred strain animal, mutant strain animal, hybrid animal, closed colony animal and mongrel. The microbiological background gives four different types: germ free animal, gaotobiotic...

The laboratory animal science is a new science. This article reports the relationship between standardization of laboratory animals and life sciences research. Biological background for the standardization of laboratory animals includs genetic background and microbiological background. The genetic background gives five different types: inbred strain animal, mutant strain animal, hybrid animal, closed colony animal and mongrel. The microbiological background gives four different types: germ free animal, gaotobiotic animal ( or gnotophorics ) , specific pathogen free animal and conventional animal. These laboratory animals of standardization have specific value of use on the biomedical research, e. g. experimental oncology, molecular genetics, immunobiology, experimental pharmacology, bioengineering and toxicology etc.

实验动物科学是一门新兴学科.本文论述了实验动物标准化与生命科学(或生物医学)研究之间的相互关系.实验动物标准化的生物学背景,包括遗传学上的背景和微生物学上的背景两个方面.实验动物从遗传学上可区分为近交系动物(也叫纯系动物)、突变种纯系动物、系统杂交动物、封闭群杂种动物和纯杂种动物5种不同的类型;从微生物学控制上可区分为无菌动物、悉生动物(或已知菌动物)、无特殊病源体动物和常规动物4大类.这些标准的实验动物,在生物医学如实验肿瘤学、分子遗传学、免疫生物学、实验药理学以及生物工程学和毒理学等研究上,都具有特殊的应用价值.

A new technique of producing animal histological section was introduced in this paper. It was improved in such part as the staining of the organ piece being made before being embedded and sectioned with the same staining effect against the common method. This new method has many good characteristics such as economizing a great deal of ethonal and xylene, simplitying the staining procedure, shorting the time of producing.

常规的动物组织石蜡切片法,通常是把动物组织块先用石蜡包埋,然后切片染色,程序繁多。本文介绍一种新的制片法,是将组织的染色、改放在组织包埋切片之前进行,其效果与切片后染色基本相同。这种方法节约了大量的乙醇、二甲苯,简化了染色程序,缩短了制片时间,为教学实验、病理检查和科学研究提供了简便易行的制片方法。

Objective To generate monoclonal antibodies against C34, a polypeptide derived from HIV-1 gp41 core structure, and to characterize the epitope of gp41 recognized by McAb. Methods McAb clones against C34 and C46 were prepared by hybridoma technique and identified for epitopes by ELISA, for effect on cell proliferation by MTT, and for effect on cell fusion by molecular fluorescence probe. Results The three of anti-C34 McAb(1G1, 2F8, 2B7) and two of anti-C46 inhibited the binding of N46 and C34. The 1G1, an anti-C34...

Objective To generate monoclonal antibodies against C34, a polypeptide derived from HIV-1 gp41 core structure, and to characterize the epitope of gp41 recognized by McAb. Methods McAb clones against C34 and C46 were prepared by hybridoma technique and identified for epitopes by ELISA, for effect on cell proliferation by MTT, and for effect on cell fusion by molecular fluorescence probe. Results The three of anti-C34 McAb(1G1, 2F8, 2B7) and two of anti-C46 inhibited the binding of N46 and C34. The 1G1, an anti-C34 McAb with the highest titer, can enhance the proliferation of H9/HIV-1ⅢB cells , but can not influence the form of syncytium conformation of HIV-1 in-fected cells(H9/HIV-1ⅢB) with uninfected cells(MT-2). Conclusion These results suggested that the epitope recognized by 1 G1 may be an enhancive epitope which can stimulate the proliferation of HIV-1 infected cell.

目的制备针对C34和C46单抗, 并以此为工具研究gp41表位,进一步 了解HIV-1包膜蛋白的作用机理和病毒的感染机制,为寻找新的治疗靶点提供抗体工具。 方法常规动物免疫、细胞融合、克隆化制备抗C34与C46的单克隆抗体,并鉴定其 特异性位点,还用ELISA法、MTT法及荧光分子探针技术对单抗的生物学活性进行研究。结果获得了3株抗C34单克隆抗体、2株抗C46单抗。此5个单抗均与C34结合 , 并抑制N36肽与C34肽复合物的形成;其中效价最高的1G1对H9/HIVⅢB细胞的生长有刺 激作用 ,对H9/HIVⅢB细胞和MT-2细胞的融合无明显影响。结论得到5株 可与C34反应,并可抑制C34与N36多肽结合的单抗, 其中1G1所识别的表位可能为增强性或刺激性表位。

 
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