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前期基因
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  “前期基因”译为未确定词的双语例句
     METHOD Using the different expression genes MMP-2 and FN, with flurence-quantitative RT-PCR and western blot methods, analysis the mRNA expression relations of MMP-2 and FN with clinical prognostic factors in 116 clinical samples and protein expression in 7 breast cancer cell lines.
     方法:采用荧光定量RT-PCR方法和Western blot方法,在前期基因芯片筛选得到的可疑转移基因MMP-2和FN的基础上,对116例临床标本和病例资料以及7个乳腺癌细胞系检测MMP-2和FN在mRNA水平和蛋白水平的表达,分析MMP-2和FN在mRNA水平和蛋白水平的表达与乳腺癌转移的关系。
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     Methods Four genes,which expressed significantly different between SLE patients and healthy controls,were found by gene chips' screening.
     方法前期基因芯片工作中筛选得到4个在55例SLE患者及正常对照之间表达差异显著的基因,染色体定位发现其位于SLE连锁的染色体区段。
短句来源
     Expressed Sequence Tags (EST) Analysis of the Heading Leaf of Chinese Cabbage (Brassica rapa L. ssp. pekinensis ) at the Early Heading Stage
     结球白菜结球前期基因表达序列标签(EST)分析
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     Differential Gene Expression between Reciprocal Cross-fertilized Kernels and Their Parents during the Early Stages of Seed Development in Wheat
     不同优势小麦正反杂交种子与亲本自交种子发育前期基因表达差异
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     Based on the gene chip's study, we focused on the analysis of nine genes related to the cell cycle regulation.
     在前期基因芯片工作的基础上,对细胞周期调控相关的9个基因进行了更深入的分析。
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     4 new genes were obtained.
     U基因
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     coli lac Z gene.
     colilacZ基因
短句来源
     Advance of Study on Effects of Chfr Gene of Mitosis Prophase Checkpoint——Review
     有丝分裂前期检查点Chfr基因作用的研究进展
短句来源
     The former studies showed that this protein was a minor structural protein.
     前期的研究结果表明,该基因产物是病毒粒子的结构蛋白。
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     Lu Xu’s Translation in His Early Period
     鲁迅的前期翻译
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  early gene
Hypobaric Hypoxia Affects Rat Behavior and Immediate Early Gene Expression in the Brain: the Corrective Effect of Preconditionin
      
The antibiotic anisomycin, a well-known stress factor, has been found to induce the transcription of immediate-early gene c-fos, which is under negative control in cells transformed by oncogenes E1A and cHa-ras.
      
Immediate-early gene product ICP22 inhibits the trans-transcription activating function of P53-mdm-2
      
As a product of HSVI immediate-early gene, ICP22 is capable of interacting with various cellular transcriptive and regulatory molecules during viral infection so as to impact the normal cellular molecular mechanism.
      
The Borf1 protein is encoded by an immediate-early gene of the bovine foamy virus (BFV) and plays a key role in the viral life cycle.
      
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fter studying of familial leukemias, Myelodysplastic Syndrome (MDS) and aplastic anemia (AA), we observed and analysed bone marrow (BM) cells hematologically and molecularcytogenetically in 36 persons who are first degree relatives (FDRs) of patients with acute leukemias (AL), MDS and AA. The peripheral blood (PB) lymphocyte chromosome fragility sensitive to folic acid and unstability was also analysed in 18 FDRs. The abnormal BM megakaryocystic/erythroid cellularity and the rearrangement of cerbB were...

fter studying of familial leukemias, Myelodysplastic Syndrome (MDS) and aplastic anemia (AA), we observed and analysed bone marrow (BM) cells hematologically and molecularcytogenetically in 36 persons who are first degree relatives (FDRs) of patients with acute leukemias (AL), MDS and AA. The peripheral blood (PB) lymphocyte chromosome fragility sensitive to folic acid and unstability was also analysed in 18 FDRs. The abnormal BM megakaryocystic/erythroid cellularity and the rearrangement of cerbB were found in 66%~86.1% of parents and siblings of patients. The associations of dysplastic megakaryopoiesis, including the presence of lymphoid small megakaryocytes, with the chromosomal monosomy or/and the rearrangement/amplification of cerbB, were found in a few parents and siblings. These results were consistent with those of MDS、 Fanconi Anemia (FA) and AL. The normal karyotype and SCD positive of BM cells and PB lymphocytes, and PB lymphocyte chromosomal fragility and unstability were found in most of patients' parents, while familial chromosomal monosomy of BM cells and PB lymphocyte chromosomal fragility were found in parents and siblings of familial leukemia patients. Based on the studies of a large family with 7 cases of acute erythroleukamia and relative myeloleukemias in three consecutive generations and a family with 3 CAA and1 AML, the rearrangement of cerbB might be inherited. The rearrangement/amplification of cerbB and its PCR detected results could be the indicators of gene diagnosis of preleukemia and might be useful in genetic conselling of leukemias. The common origin of AL, MDS and AA was discussed.

在长期研究家族性白血病及其前期的基础上,应用相同的血液学和细胞遗传学指标及Southern印迹杂交技术,对20例急性白血病及其前期患者(儿)父母或兄弟姐妹等一级亲属骨髓和外周血进行研究,发现散发性白血病及其前期同家族性白血病及其前期一样,与患者(儿)父母骨髓巨核系、红系或/和粒系异常增生相关。家族性白血病及其前期患者(儿)父母一方或双方还有家族性骨髓细胞染色体单体性即非随机丢失或外周血显示淋巴细胞叶酸敏感型染色体脆性部位的高表达;而散发性白血病及其前期患者(儿)父母多数骨髓细胞核型正常,但其外淋巴细胞则表现出多种类型的染色体异常。鉴于以往已证明家族性骨髓巨核系和红系异常增生及其相关的cerbB重排的可遗传性,以及骨髓病态造血与cerbB重排扩增的相关性,除了cerbB重排扩增以及VerbBPCR检测结果可作为白血病前期基因诊断指标外,cerbB重排及其相关的骨髓异常增生看来还可用于婚前检查、产前诊断和遗传咨询服务等,以便对白血病进行预防。

In order to understand the molecular basis of heterosis, modified differential display of mRNA was used to characterize the difference in gene expression between cross-fertilized and self-fertilized kernels at 2, 4, 6, 8, 10 and 12 d after pollination (DAP) by using 3 wheat (Triticum aestivum) hybrids with different levels of heterosis. The results indicated that the patterns of gene expression altered obviously in cross-fertilized kernels as compared to self-fertilized kernels. Both quantitative and qualitative...

In order to understand the molecular basis of heterosis, modified differential display of mRNA was used to characterize the difference in gene expression between cross-fertilized and self-fertilized kernels at 2, 4, 6, 8, 10 and 12 d after pollination (DAP) by using 3 wheat (Triticum aestivum) hybrids with different levels of heterosis. The results indicated that the patterns of gene expression altered obviously in cross-fertilized kernels as compared to self-fertilized kernels. Both quantitative and qualitative differences were observed. Major difference of gene expression occurred in the qualitative level. The further analysis showed that higher percentage of differentially expressed cDNA was observed in highly heterotic hybrid. As far as MADS-box concerned, with the development of seed, there were gradually higher percentage of differentially expressed cDNA in Nongda3159 × Jingdong6, Nongda3159 × Yuandong8790 and Nongda3159 × Nongda3214 as compared to self-fertilized Nongda3159, but decreased at 4 DAP, and the cross-fertilization effect was maximum at 10 DAP. As for Ser/Thr gene family,there were gradually higher percentage of differentially expressed gene at the 2 DAP, 4 DAP and 6 DAP, then decreased, and the cross-fertilization effect was maximum at 6 DAP.

为了探讨小麦(Triticumaestivum)杂种优势形成的分子机理,选用杂种优势不同的3个杂交组合,以授粉后2~12 d的杂交和自交的种子为材料,采用mRNA差异显示技术分析了MADS-box和Ser/Thr蛋白激酶两个基因家族在不同优势组合的杂交和自交种子发育前期的基因表达差异。结果表明,所有6个时期杂交和自交种子之间存在明显的基因表达差异,表现为量和质的两类差异。进一步分析发现,强优势组合差异表达比例较弱优势组合高。3个杂交组合农大3159×京冬6号、农大3159×原冬8790和农大3159×农大3214与农大3159自交相比,MADS-box家族基因的差异表达比例随发育时期呈一定的递增趋势,但在授粉后4和12 d时,差异表达的比例明显减少;Ser/Thr蛋白激酶家族基因差异表达比例在授粉后2 、4 和6 d时随发育时期呈递增趋势,6 d时差异表达达到最大,后逐渐减小。

To understand the molecular basis of heterosis, modified differential display of mRNA was used to characterize the difference in gene expression between reciprocal cross-fertilized kernels and their parents seeds at 2, 6 and 12 days after pollination (DAP) by using 3 wheat hybrids with different level of heterosis.The results indicated that gene expression altered obviously in reciprocal cross-fertilized kernels as compared to their parents.The patterns of differential expression included:(1) Gene specifically...

To understand the molecular basis of heterosis, modified differential display of mRNA was used to characterize the difference in gene expression between reciprocal cross-fertilized kernels and their parents seeds at 2, 6 and 12 days after pollination (DAP) by using 3 wheat hybrids with different level of heterosis.The results indicated that gene expression altered obviously in reciprocal cross-fertilized kernels as compared to their parents.The patterns of differential expression included:(1) Gene specifically expressed in cross-fertilized kernels but not in both parents; (2) Gene expressed in both parents but not in the cross-fertilized kernels and gene expressed in only one parents but not in the reciprocal cross-fertilized kernels; (3) Gene Identically expressed with one parent.The further analysis showed that higher percentage of sliencing expressed cDNA and lower percentage of identically expressed cDNA were observed in highly heterotic hybrid, which might be responsible for the observed heterosis.

为探讨小麦杂种优势形成的分子机理 ,本研究选用优势不同的 3个杂交组合 ,以授粉后 2、6和 12d的正反杂交种子与亲本自交种子为材料 ,采用mRNA差异显示技术分析了不同优势正反杂交种子与亲本自交种子之间发育前期的基因表达差异。结果表明 ,所有 3个时期正反杂交种子与亲本自交种子之间存在明显的基因表达差异 ,差异可归纳为特异型 (SPE)、沉默型 (SIE)和单亲表达 (IEP) 3大类型 ,且随着优势的减弱 ,沉默表达所占比例增大 ,而单亲表达所占比例减少。

 
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