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极性细胞
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  “极性细胞”译为未确定词的双语例句
     There are microvilli on the cell surface and tight connections between the adjacent cells.
     细胞存在极性,细胞尖端存在短的微绒毛,细胞之间连接紧密。
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  相似匹配句对
     Roles of Cell Wall in Cell Polarity Establishment and Embryogenesis
     细胞壁在细胞极性建立和胚胎发生中的作用
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     Tom Clancys Splinter Cell
     细胞分裂
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     The asymmetric distribution of Numb is controlled by the cell polarity molecules which accumulate in the appropriate pole of the cell before mitosis.
     Numb不对称分布由细胞极性分子控制。
短句来源
     ALBUMINOUS CELLS
     蛋白细胞
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     On the Thermoelasticity of Polar Material
     极性材料的热弹性力学
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  polarized cell
Results from epithelial model systems have shown that the polarized cell phenotype is generated by specific protein sorting and regulated protein trafficking between the trans-Golgi network and the cell surface.
      
Evidence for a branched pathway in the polarized cell division of Saccharomyces cerevisiae
      
Following intracellular acidification caused by withdrawal of NH4+, pHi recovery also was slightly slower than in the normally polarized cell.
      
Before fertilization, the synergid is a highly polarized cell with dense longitudinally aligned arrays of microtubules adjacent to the filiform apparatus at the micropylar end of the cell associated with major organelles.
      
Before fertilization, the synergid is a highly polarized cell with dense longitudinally aligned arrays of microtubules adjacent to the filiform apparatus at the micropylar end of the cell associated with major organelles.
      
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Objective To observe the bionomics of iris pigment epithelium (IPE)cells of rabbits,in order to establish basis for treating age-related macular degeneration through IPE transplantation method.Methods The ultrastructure of cultured IPE cells was observed by transmission electron microscopy and scanning electron microscopy,and the bionomics of IPE cells was investigated by measuring theirs plating efficiency,spreading rate,doubling time,population doubling and growth curve.Results Lots of fibrils of varied lengths...

Objective To observe the bionomics of iris pigment epithelium (IPE)cells of rabbits,in order to establish basis for treating age-related macular degeneration through IPE transplantation method.Methods The ultrastructure of cultured IPE cells was observed by transmission electron microscopy and scanning electron microscopy,and the bionomics of IPE cells was investigated by measuring theirs plating efficiency,spreading rate,doubling time,population doubling and growth curve.Results Lots of fibrils of varied lengths and diameters were present on the surface of the IPE cells,and plenty of organelles and pigment granules were found in IPE cells' cytoplasm.IPE cells in vitro were cubical-shaped and grew into an apical-basal polarized monolayer.Spreading rates of IPE was 81.5%±3.7%;The plating efficiencies of primary IPE cells and subcultured IPE cells were 32.18%±3.18% and 86.20%±2.84% respectively.The average doubling time was 2~7 days (mean 3.46 days±2.10 days) and their population doubling was 6~10 generations.Conclusion IPE cells cultured in vitro had similar bionomics to cells grown in vivo.Research of bionomics in iris pigment epithelium of rabbit in vitro could provide an important experimental basis for further functional research and autologous transplantation.

目的观察体外培养兔眼虹膜色素上皮(irispigmentepithelium,IPE)细胞的生物学特性,为IPE移植治疗老年性黄斑变性等疾病打下基础。方法应用扫描电镜、透射电镜对所培养IPE细胞的超微结构进行观察,并通过细胞的贴壁率、伸展率、倍增时间、总分裂次数的测定以及生长曲线的绘制对其在体外生长的生物学特性进行研究。结果体外培养的IPE细胞呈多角形,细胞内有色素颗粒和丰富的细胞器,细胞表面有长短粗细不等的微绒毛,生长成为具有尖基底极性的细胞单层。IPE细胞伸展率为81.5%±3.7%;原代IPE细胞的贴壁率为32.18%±3.18%;传代培养的IPE细胞的贴壁率为86.20%±2.84%;细胞倍增时间为2~7d,平均为(3.46±2.10)d;细胞总分裂次数为6~10代。结论体外培养IPE细胞与体内生长IPE细胞有着相似的生物学特性,通过对IPE细胞体外生物学特性的研究,为进一步探索其功能和自体移植提供了必要的实验基础。

The Rho family proteins are major members of the Ras superfamily small GTPases,conserved in the eukaryotic cells.The Rho GTPase family consists of subfamily members of Rho,Rac and Cdc42,which act as molecular switches in several signal transduction pathways,such as in the regulation of cell polarity,cell movement,cell shape,and cell-cell and cell-matrix interaction.To understand the function and evolution of the Rho family proteins in Magnaporthe grisea and other fungi,we searched the M.grisea genome database,and...

The Rho family proteins are major members of the Ras superfamily small GTPases,conserved in the eukaryotic cells.The Rho GTPase family consists of subfamily members of Rho,Rac and Cdc42,which act as molecular switches in several signal transduction pathways,such as in the regulation of cell polarity,cell movement,cell shape,and cell-cell and cell-matrix interaction.To understand the function and evolution of the Rho family proteins in Magnaporthe grisea and other fungi,we searched the M.grisea genome database,and retrieved more than 30 small GTPase homologous proteins,and 7 putative Rho family proteins were confirmed by analyzing the presence of a set of conserved G box GDP/GTP-binding motif elements(G1-G5).We further analyzed the enconding gene expression of these proteins during the growth and development stages in M.grisea by semi-quantitive RT-PCR,and results showed that these genes have different expression(patterns).We also constructed a phylogenetic tree of Cdc42,Rac1 and Rho1 of fungi based on the sequences(available) in GenBank,the tree well reflected the evolutionary relationship of fungi.We concluded that the structure and the function of Rho family proteins have co-evoluted through the long evolutionary process of fungi.

Rho族蛋白是Ras超家族小G蛋白最主要成员,在真核生物中极为保守,包括Rho、Rac、Cdc42等,作为分子开关,参与细胞极性、细胞运动、细胞形状、细胞间及细胞与其基质互作等多个信号转导途径。基因组数据库分析显示,稻瘟菌中含有30余个小G蛋白,其中7个可能是Rho族蛋白,包括Cdc42、Rac1及5个Rho亚家族蛋白。结构分析表明,7个推导的Rho族蛋白均具有典型结构域。进一步以半定量RT-PCR分析了稻瘟菌7个Rho族蛋白编码基因在菌丝、分生孢子、芽管以及附着胞等不同生长发育阶段的表达情况。结果表明,Rho族蛋白编码基因具有不同的表达模式。通过BLASTP搜索GenBank等数据库,获得迄今已公布的真菌Rho1、Cdc42和Rac1蛋白序列,建立了系统发育树,一定程度地反映了真菌进化关系,说明在真菌进化中Rho族蛋白的结构与功能也经历了共同演化的过程。

 
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