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肿瘤学
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S细胞
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  s cells
     After the transfection of MDA-MB-435S(human) breast carcinoma cells with STAT3 decoy oligodeoxynucleotides(Decoy ODNs),expressions of HSP27 and c-MYC of MDA-MB-435S were semi-quantified by Western blot,the apoptosis and the cell cycle distribution of MDA-MB-435S cells were analyzed with flow cytometry and proliferation of MDA-MB-435S cells was analyzed by MTT assay.
     在体外应用诱骗寡核苷酸(Decoy ODNs)抑制乳腺癌细胞MDA-MB-435S STAT3活性,W estern b lot检测MDA-MB-435S细胞HSP27,c-MYC蛋白表达,MTT检测肿瘤细胞增殖力,FCM检测MDA-MB-435S细胞周期和凋亡.
短句来源
     Hodgkin′s disease: Ig gene rearrangements in single H/Rs cells
     Hodgkin病单个H/R-S细胞Ig基因重排的研究
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     Results:MTT assay that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration (IC_50 ) was 0.128mg/L.
     结果:ADR抑制MCF7/S细胞增殖,呈剂量依赖性,IC50为0.128mg/L;
短句来源
     ConclusionWith the toxic dose, PVC can inhibite the proliferation of MCF - 7/S cells and MCF - 7/R cells in the manner of dose/time - dependent and induce the apoptosis of MCF - 7/S cells and MCF - 7/R cells;
     MTT法检测不同浓度PVC对MCF-7/S细胞和MCF-7/R细胞增殖的影响以及非细胞毒剂量PVC对MCF-7/R细胞阿霉素耐药性的影响。
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     Results The expression of ABC gene family was positive in MCF 7/ADM cells compared to MCF 7/S cells;
     结果 ABC家族基因在MCF 7/ADM细胞中均过表达 ,而在MCF 7/S细胞中均未见表达 ;
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  sternberg cells
     Immunohistochemistry for CD30 and CD15 was performed with SABC method, and the positive expression rate of Reed Sternberg cells for CD30 and CD15 was investigated in different subtypes as well as in each case.
     将 32例 c HL全部按 WHO新分类标准进行病理分型 ,采用免疫组织化学 SABC法进行 CD30和 CD15染色 ,观察每一病例及不同亚型 R- S细胞阳性表达率。
短句来源
     The different expression of Reed sternberg cells for CD30 or CD15 might be related to the heterogenicity of neoplastic cells.
     CD15和 CD30可作为诊断 c HL的参考依据 ,在 R- S细胞中的表达差异可能与肿瘤细胞的异质性有关
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     To study the different expression of CD30 and CD15 of Reed Sternberg cells in classical Hodgkin's lymphoma (cHL) and its subtypes, all 32 CHL cases were divided into four subtypes according to the new pathological classification.
     探讨经典型霍奇金淋巴瘤 (c HL )及其亚型的 R- S细胞 CD30、CD15蛋白表达的差异性。
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  “S细胞”译为未确定词的双语例句
     2.The appearance of VH1,VH2,VH3,FR2a,FR3 gene rearrangements suggested that the H/R-S cells from classical HL(NSHL and MCHL)originated from B lineage cells at various stages of differentiation.
     2.VH1、VH2、VH3、FR2a、FR3基因重排的出现,提示NSHL和MCHL的H/R-S细胞来源于不同分化阶段的B细胞,NSHLa同时出现FR1、FR3基因重排;
短句来源
     2.VH2,VH3 and FR2a gene rearrangements were revealed in one case of mixed cellarity HL(MCHL).
     1例混合细胞型HL(MCHL)的H/R-S细胞有VH2、VH3、FR2a基因重排。
短句来源
     Research of H/R-S cells chacter with 5′-labeled direct in situ PCR
     5′-标记引物直接原位PCR对H/R-S细胞性质的研究
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     Objective To study whether shRNA-CXCR4 could inhibit the CXCR4 gene expression in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells.
     目的探讨shRNA-CXCR4对人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435 s细胞CXCR4基因表达及蛋白表达的影响。
短句来源
     Analysis of TCR-γ gene rearrangement in single H/R-S cells of Hodgkin disease
     霍奇金病单个H/R-S细胞TCR-γ基因重排的研究
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  s cells
Ultrastructural Changes in Interstitial Cajal's Cells and Myocytes of the Small Intestine of Suckling Rabbits with Experimental
      
12S cells grown on the substrate mixture were 1.1 to 1.7 times lower than they were during growth on ethanol alone.
      
Since this chromosome was present in only a proportion of patient's cells studied and no chromosome aberrations were revealed in cells of his parents, the der(3)t(3;10) is suggested to appear de novo.
      
This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry.
      
Fractional shortening examined at 0.25 Hz was 14.67±0.51 % in S cells and was not significantly different from U cells.
      
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  secretin cell
Ultrastructural and immunofluorescent investigations on the secretin cell in the dog intestinal mucosa
      
These targetoid granules represent a distinctive ultrastructural marker of the secretin cell.
      
Secretin cell granules have been shown to react with chromogranin A antibodies and Grimelius' silver, while lacking chromogranin B immunoreactivity.
      
Distribution, ontogeny and ultrastructure of the mammalian secretin cell
      
Immunohistochemical and ultrastructural detection of the secretin cell in the pig intestinal mucosa
      
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  sternberg cells
Npm/alk fusion mRNA expression in Hodgkin and Reed-Sternberg cells is rare but does occur: Results from single-cell cDNA analysi
      
Background: Reed-Sternberg cells of Hodgkin's disease express CD30and CD40 receptors.
      
Npm/alk fusion mRNA expression in Hodgkin and Reed-Sternberg cells is rare but does occur: Results from single-cell cDNA analysi
      
Background: Reed-Sternberg cells of Hodgkin's disease express CD30and CD40 receptors.
      
Clonality and germinal centre B-cell derivation of Hodgkin/Reed-Sternberg cells in Hodgkin's disease
      
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Sixteen mutants resistant to ouabain,which inhibits the plasma membrane Na~++K~+-ATPase,have been isolated fromWg3-h.Ouabain at concentrations of 0.5mM with 3.0 mM K~+,or 0.025 mMwith 0.3 mM K~+,inhibits the growthof the wildtype cells and is ultimatelycytotoxic.Clones 10-fold more resistantthan wild-type in terms of dose wereobtained by single-step selection from awild-type population in the presence ofouabain.The phenotypes of ouabain-re-sistant(Ouα~R)clones are reproduciblewith high fidelity and stable over...

Sixteen mutants resistant to ouabain,which inhibits the plasma membrane Na~++K~+-ATPase,have been isolated fromWg3-h.Ouabain at concentrations of 0.5mM with 3.0 mM K~+,or 0.025 mMwith 0.3 mM K~+,inhibits the growthof the wildtype cells and is ultimatelycytotoxic.Clones 10-fold more resistantthan wild-type in terms of dose wereobtained by single-step selection from awild-type population in the presence ofouabain.The phenotypes of ouabain-re-sistant(Ouα~R)clones are reproduciblewith high fidelity and stable over one-year interval of growth in the absenceof the selecting compound.Wild-typeand Ouα~R Wg3-h cell clones were com-pared with respect to their susceptibilityto ouabain inhibition of K~+ uptake bywhole cells.Ouα~R cells are less sensitiveto ouabain inhibition on active Na~+,K~+transport than are wild type cells.Ouα~Rcells are fully sensitive to HAT mediumand digitoxin just like the cells of theoriginal cell line.There are no signifi-cant differences in growth rate and re-quirement of growth conditions betweenthe Ouα~R cells and Ouα~S cells. The morphological features were ob-served by scanning electronic microscopy.The nature of Ouα~R is discussed withthe result of 2 D-gel electrophoresis. The Ouα~R marker can be useful forselection of somatic cell hybrids.

乌本苷是质膜Na~+、K~+ATP酶的特异性抑制剂。在3.0 mM K~+的培养液中,0.5mM浓度的乌本苷可完全抑制野生型Wg 3-h细胞的生长;而在0.3 mM K~+的培养液中,则只需0.025 mM的药物浓度即可。以5 mM乌本苷为选择标准,本文作者以一步群选法从Wg 3-h细胞中选出16个抗性克隆(Ouα~R)。Ouα~R表型稳定,在无药物选择压力长达一年之久的情况下仍保持不变。比较Ouα~s和Ouα~R细胞的Na~+/K~+运输,发现Ouα~R细胞对乌本苷抑制作用的敏感性降低。Ouα~R细胞与Ouα~s细胞两者在生长速度、对培养液的需求,对HAT培养液和洋地黄毒苷的敏感性方面,均无明显差异。运用扫描电镜技术,观察到一些在光学显微镜下所见不到的乌本苷对细胞的影响。本文还就蛋白质双向电泳分析的结果,对Ouα~R的本质作了探讨。Ouα~R遗传标记,可用于体细胞融合中杂种细胞的筛选。

18 cases of malignant lympomas were studied by using ABC immunohisto- logical method with various monoclonal and polyclonal antisera.In all cases it was found staining with OKT9 antiseram positive,while cytokeratin nega- tive.Among B cell lymphomas 9 cases,all cases staining with B1 antiserum, 7 cases with I_2 and Leu 10,6 cases with J5 and 5 cases with B2 were posi- tive.Among T cell lymphoma 6 cases,5 cases staining with T 3 and Tac,4 cases with T11,2 cases with T6 were positive while all cases with T4 posi-...

18 cases of malignant lympomas were studied by using ABC immunohisto- logical method with various monoclonal and polyclonal antisera.In all cases it was found staining with OKT9 antiseram positive,while cytokeratin nega- tive.Among B cell lymphomas 9 cases,all cases staining with B1 antiserum, 7 cases with I_2 and Leu 10,6 cases with J5 and 5 cases with B2 were posi- tive.Among T cell lymphoma 6 cases,5 cases staining with T 3 and Tac,4 cases with T11,2 cases with T6 were positive while all cases with T4 posi- tive and T8a negative or weakly positive.There are 1 cases of histiocytic lym- photon and 2 cases of Hodgkin's lymphoma showing positive reaction with Mol, 2H9 and lysozyme antibody in their tumor cells and R-S cells respectively. Based on the results the cytogenesis,routine diagnosis and prognosis of malign- ant lymphomas were discussed.

本文应用较多种单克隆和多克隆抗血清,采用 ABC 法对18例恶性淋巴瘤进行标记,其结果 OKT_9均呈阳性而 Cytokeratin 均呈阴性。B 细胞淋巴瘤有9例,B_1均呈阳性,B_2~+5例、I_2~+和 Leu 10~+各7例,J_6~+6例。T 细胞淋巴瘤有6例,T_4均呈阳性而 T_(8A)呈阴性,T_3~+5例,Tac~+5例、T_(11)~+4例、T_6~+2例。组织细胞性淋巴瘤有1例,何杰金氏淋巴瘤有2例,其瘤细胞和 R—S 细胞的Mol、2H_9和溶菌酶均呈阳性。根据标记结果对恶性淋巴瘤的细胞来源、常规切片诊断和预后等问题进行了初步分析。

Using the histochemical and immunohistochemical methods,eight adult hu- man pituitary glands from autopsy cases were studied.The adenohypophysis were composed of five distinct types of cells,which produced at least six diff- erent kinds of hormones,showing different characteristics in their morphology, number,distribution and histochemical reaction,The acidophilic cells consi- sted of GH cells and PRL cells,The basophilic cells consisted of ACTH, TSH and GonT cells.The PFA-AB-PAS-OG method showed that most of...

Using the histochemical and immunohistochemical methods,eight adult hu- man pituitary glands from autopsy cases were studied.The adenohypophysis were composed of five distinct types of cells,which produced at least six diff- erent kinds of hormones,showing different characteristics in their morphology, number,distribution and histochemical reaction,The acidophilic cells consi- sted of GH cells and PRL cells,The basophilic cells consisted of ACTH, TSH and GonT cells.The PFA-AB-PAS-OG method showed that most of chromophobic cells were alcianophilic and immunohistochemically positive for TSH,LH or FSH.A pronounced hyperplasia of PRL cells accompanied by decrease of GH cells was noticed in a pregnant woman's adenohypophys- is.Some of the follicular epithelial cells and those arranged in“small folli- cles”in pars distalis were chromophilic and contained different types of hor- mones.It was supposed that the follicular epithelial cells might directly differentiate into any type of mature hormone-producing cells.The sensitivity,specificity and reproducidility of the lmmunohistochemical method which is suitable for the study of the functional classification of adenohypophyseal cells are demonstrated,

应用免疫组化对腺垂体细胞进行分型能较好地反映各型激素细胞形态与功能的统一关系,观察结果表明免疫组化 PAP 法敏感特异且重复性强,效果明显优于普通组织化学染色。嗜酸性细胞包括 GH 和 PRL 细胞。嗜碱性细胞包括 R 细胞(含 ACTH)和 S 细胞(含 TSH,LH,与 FSH)。部份滤泡上皮细胞可含有各种不同的激素物质,沪泡上皮多嫌色性。

 
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