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稳态RNA
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  相似匹配句对
     RNA of changing with each passing day
     日新月异的RNA
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     RNA Folding
     RNA折叠
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     Steady-State Surface Photovoltage Technique
     稳态光电压测量技术
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     Steady-state sound field in enclosures
     室内稳态声场
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Objective To investigate the influence of adenovirus major late tripartite leader sequence on specific exportation of virus mRNA in the late phase of adenovirus infection.Methods We constructed a marked, human beta actin minigene under the control of the glucocorticoid inducible enhancer and promoter of mouse mammary tumor virus, and inserted it into the left end of the adenovirus type V genome. The promoter gene, designated MA, as well as of a sibling, which differed only in inclusion of a cDNA copy of...

Objective To investigate the influence of adenovirus major late tripartite leader sequence on specific exportation of virus mRNA in the late phase of adenovirus infection.Methods We constructed a marked, human beta actin minigene under the control of the glucocorticoid inducible enhancer and promoter of mouse mammary tumor virus, and inserted it into the left end of the adenovirus type V genome. The promoter gene, designated MA, as well as of a sibling, which differed only in inclusion of a cDNA copy of the adenovirus major late tripartite leader sequence upstream of beta actin sequences termed MtplA, in recombinant virus infected cells was strictly dependent on addition of deoxymethesone to the medium. Nuclear and cytoplasmic reporter RNA species were compared by Northern blotting, primer extension and pulse chase assay.Results The high concentrations of newly synthesized RNA were accumulated in cytoplasm when tripartite leader sequence was present in reporter RNA, despite the equal rates of transcription of the two reporter genes. Nuclear and cytoplasmic reporter RNA species compared by Northern blotting, primer extension, and pulse chase provided no evidence for altered processing induced by tripartite leader sequence. Conclusion The tripartite leader sequence, known to facilitate translation of mRNA during the late phase of adenovirus infection, can also modulate mRNA export from the nucleus.

目的观察腺病毒主要晚期三联先导序列(tripartiteleadersequence,tpl)在腺病毒C型感染细胞晚期对病毒mRNA转运的影响。方法用构建的激素诱导型腺病毒重组体MA及MtplA(含有腺病毒主要晚期先导序列)感染细胞,采用Northern印迹、引物延伸及脉冲示踪等方法比较感染细胞中特异性报告基因β肌动蛋白mRNA的转送特征。结果尽管两种报告基因转录产物的合成速率相同,但在含有tpl的MtplA腺病毒感染的细胞中,胞浆内报道基因mRNA浓度更高。结论对细胞核与细胞质之间MA和MtplA的新合成和稳态RNA的分析表明,tpl并不能增加病毒mRNA在胞质中的稳定性,但可促进病毒mRNA从胞核到胞质的转送

 
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