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近汇合
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  subconfluent
     Sensitivity to TSH was greater in adherent monolayers of cells rather than cell suspensions and was also greater in subconfluent rather than confluent monolayers of cells.
     其反应性在细胞单层高于细胞是液,近汇合细胞单层高于汇合细胞单层。
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  “近汇合”译为未确定词的双语例句
     RESULTS; ①In cytotoxic test, L-929 cells soaked in Ti materials for 7 days were confluenced in normal morphology and regular arrangement;
     结果:①细胞毒性评价:3种钛金属材料浸泡7d时L-929细胞形态正常,生长近汇合,排列密集规则;
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  相似匹配句对
     10.near to far.
     (10)从到远;
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     Although D.
     在缘种D.
短句来源
     Sensitivity to TSH was greater in adherent monolayers of cells rather than cell suspensions and was also greater in subconfluent rather than confluent monolayers of cells.
     其反应性在细胞单层高于细胞是液,汇合细胞单层高于汇合细胞单层。
短句来源
     Everything That Rises Must Converge
     上升的一切必将汇合
短句来源
     Converging Theories in Linguistics
     语言学中的汇合理论
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  subconfluent
Moreover, SEM analysis performed on cold plasma modified specimens showed the presence of a subconfluent monolayer of EC, with an elongated spread morphology.
      
The Sykes-Moore and Rose chambers have been used for many years in time lapse studies of subconfluent cell cultures.
      
Human subconfluent keratinocytes were labeled with fluorescence cell linker and cultured in two brands of fibrin glue (TissucolR and BeriplastR) in vitro for 5 days.
      
Growth-stimulatory activities for subconfluent fibroblasts in 10% test sera were measured by increased cell numbers; again, there was no significant difference between scleroderma-affected and normal controls.
      
Autocrine growth of human epidermal keratinocytes can be maintained in subconfluent cell cultures in the absence of exogenous growth factors.
      
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The effects of thyrotrophin(TSH)and carbachol(Cch)on intracellular free calcium concentration ([Ca~(2+)]_1)in primary cultures of porcine thyroid cells were examined.TSH and Cch caused an acute increase in[Ca~(2+)]_1 in porcine cells.The effect was mediated by the respective receptor.Responses were seen at concentration of TSH between 0.01 and 10 mU/ml,and at concentration of Cch between 10~(-5) and 10~(-4)mol/L.The sensitivity to TSH or Cch was greater in adherent monolayers of cells than that of cell suspensions...

The effects of thyrotrophin(TSH)and carbachol(Cch)on intracellular free calcium concentration ([Ca~(2+)]_1)in primary cultures of porcine thyroid cells were examined.TSH and Cch caused an acute increase in[Ca~(2+)]_1 in porcine cells.The effect was mediated by the respective receptor.Responses were seen at concentration of TSH between 0.01 and 10 mU/ml,and at concentration of Cch between 10~(-5) and 10~(-4)mol/L.The sensitivity to TSH or Cch was greater in adherent monolayers of cells than that of cell suspensions and was also greater in subconfluent monolayers of cells than that of confluent ones. Both TSH and Cch were able to transiently increase[Ca~(2+)]_1 in Ca~(2+)free/EGTA buffer,indicating that the increased[Ca~(2+)]_1 originated in the mobilization of[Ca~(2+)]_1 stored in the cells.

研究促甲状腺激素(TSH)和 Carbachol(Cch)对猪甲状腺细胞 Ca~(2+)的动员作用的结果表明,0.01~10mU/ml TSH 或10~(-5)~10~(-4)mol/L Cch 可引起甲状腺细胞[Ca~(2+)]急性升高.甲状腺细胞对 TSH 或 Cch 反应的敏感性在贴壁的单层细胞高于细胞悬液,在近汇合单层细胞高于汇合单层细胞。TSH 和 Cch 均能在无钙/EGTA 缓冲介质中刺激猪甲状腺细胞[Ca~(2+)]升高,提示此升高的[Ca~(2+)]源自细胞内储藏 Ca~(2+)的动员。

The effects of thyrotrophin (TSH)and forskolin were examined on intrace-llular free calcium ([Ca2+]l)and calmodulin in normal pig thyroid cells in primary culture.TSH was found to produce acute increases in[Ca2+]l in pig cells.Responses were seen at concentrations of TSH between 0.01 and 10mU/mL.Sensitivity to TSH was greater in adherent monolayers of cells rather than cell suspensions and was also greater in subconfluent rather than confluent monolayers of cells.The increase in [Ca2+], in response to TSH represented...

The effects of thyrotrophin (TSH)and forskolin were examined on intrace-llular free calcium ([Ca2+]l)and calmodulin in normal pig thyroid cells in primary culture.TSH was found to produce acute increases in[Ca2+]l in pig cells.Responses were seen at concentrations of TSH between 0.01 and 10mU/mL.Sensitivity to TSH was greater in adherent monolayers of cells rather than cell suspensions and was also greater in subconfluent rather than confluent monolayers of cells.The increase in [Ca2+], in response to TSH represented just over a doubling in [Ca2+]l whether examined at 22C or 37C.Forskolin falied to affect [Ca2+]l TSH increased [Ca2+]l in the absence of extracellular calcium.TSH, but not forskolin, produced a significant increase in intracellular calmodulin after 3 days of culture of cells with TSH.The increase in calmodulin was of the order of 60% and did not relate to any effect of TSH on thyroid cell number.

本文研究TSH和forskolin对原代培养的猪甲状腺细胞[Ca~(2+)]_i和钙调蛋白的影响。结果表明,TSH可引起甲状腺细胞[Ca~(2+)]_1急性升高。此反应是剂量依赖关系,而与细胞外钙的存在与否无关。其反应性在细胞单层高于细胞是液,近汇合细胞单层高于汇合细胞单层。TSH作用3天,可使甲状腺细胞的钙调蛋白含量增高,此作用与TSH对甲状腺细胞数的影响无关。Forskolin对甲状腺细胞的[Ca~(2+)]_i和钙调蛋白均无明显的影响。

AIM: To evaluate cell compatibility and biocompatibility of Ti-6Al-7Nb alloy. METHODS; The experiment was carried out in the Department of Oral Biology, Stomatological College of the Fourth Military Medical University of Chinese PLA from May to October of 2006.①Cytotoxic test: According to National Standard of Medical Appliance (GB/T16886), the cytotoxic test was conducted in vitro among 5 groups: Ti-6Al-7Nb alloy group, Ti-6Al-4V alloy group, pure Ti group, pure plumbi group and blank group. L-929 cells at...

AIM: To evaluate cell compatibility and biocompatibility of Ti-6Al-7Nb alloy. METHODS; The experiment was carried out in the Department of Oral Biology, Stomatological College of the Fourth Military Medical University of Chinese PLA from May to October of 2006.①Cytotoxic test: According to National Standard of Medical Appliance (GB/T16886), the cytotoxic test was conducted in vitro among 5 groups: Ti-6Al-7Nb alloy group, Ti-6Al-4V alloy group, pure Ti group, pure plumbi group and blank group. L-929 cells at logarithmic growth phase were used to prepare the cellular suspension of 1×107 L-1 after trypin digestion and then were incubated on plate, which was followed by adhesive growth of cells, removal of primary medium, and wash with buffer phosphate. Then leaching liquor by four kinds of materials and DMEM were added respectively. Inverted phase contrast microscope was used to determine cellular morphology at days 1, 3, 5, 7 of culture. Each well of culture plate containing MTT was washed with buffer phosphate, added with dimethyl sulfoxide and leaching liquor was extracted. Enzyme-Linked Immunoassay was adopted for absorbance value (A value) at the wave length of 490 nm. Then relative proliferation rate of cells was calculated according to the formula of (A value of trial group/A value of blank group) ×100%. Cytotoxicity was ranked as 0, 1, 2, 3, 4 grades corresponding to relative proliferation rate ≥100%, 80%-99%, 50%-79%, 30%-49%, 0-29%.②Acute hemolysis test: Fresh anticoagulated and attenuated blood was sampled from 10 mL New Zealand rabbits. Ti-6Al-7Nb alloy and Ti-6Al-4V alloy of each 5 g were soaked into tube containing 10 mL saline, while distilled water and saline of each 10 mL into positive control group and negative control group, respectively. Supernatant liquid was removed after centrifugation. Spectrophotometer was used to assay A value and then hemolysis rate was calculated by (Dt-Dnc)/(Dpc-Dnc)×100% (Dt for alloy group, Dnc for negative control group, Dpc for positive control group).③Short-term subcutaneous implantation test: Implantation material was prepared in bladder between subcutaneouly and muscle in 10 New Zealand rabbits, the length from bladder bottom to incision was more than 10 mm and the interval between the two bladders was more than 10 mm, in order to prevent the contact. Each rabbit was implanted with four kinds of materials, one for each kind. Three rabbits were executed after anesthesia at weeks 1, 4, 12 post-operation to conduct hematoxylin-eosin staining in the surrounding tissues of materials and observe the number and type of inflammatory cells, together with thickness of fibrous coating membrane, which were all indices of tissue reaction degree. RESULTS; ①In cytotoxic test, L-929 cells soaked in Ti materials for 7 days were confluenced in normal morphology and regular arrangement; A large amount of cells died in pure plumbi group. The relative proliferation rates of Ti-6Al-7Nb alloy group, Ti-6Al-4V alloy group, pure Ti group, and pure plumbi group were 100%, 97%, 101% and 9% respectively. The level of cytotoxicity was grade 0 in Ti-6Al-7Nb alloy group and pure Ti group, grade 1 in Ti-6Al-4V alloy group, and grade 4 in pure plumbi group.②The hemolysis degree of Ti-6Al-7Nb alloy and Ti-6Al-4V alloy was 0.95% and 1.08%, which was lower than 5%, the national standard. No obvious acute hemolysis appeared.③In the subcutaneous implantation test, the fibrous membrane coated with Ti materials was transparent and thin, whereas that with pure plumbi was translucent and ivory white. And 12 weeks post-operation, the inflammatory cells surrounding Ti materials were lower in density, coating membrane became thinner. The number of inflammatory cells and coating thickness in pure plumbi group was higher than those of Ti material groups, and the tissue reaction was more serious. The inflammatory cell/tissue reaction degree was grade Ⅰ in Ti-6Al-7Nb alloy group and pure Ti group, grades Ⅰ and Ⅱ in Ti-6Al-4V alloy group, and grades Ⅱ and Ⅲ in pure plumbi group. CONCLUSION; Ti-6Al-7Nb alloy having good cell compatibility and biocompatibility is an ideal biomedical titanium alloy.

目的:评价Ti-6Al-7Nb合金的细胞相容性和组织相容性。方法:实验于2006-05/10在解放军第四军医大学口腔生物学实验室完成。实验分组:①细胞毒性试验:按照GB/T16886.5-2003《医疗器械生物学评价》体外细胞毒性的试验方法进行。分为5组:Ti-6Al-7Nb合金组、Ti-6Al-4V合金组、纯钛组、纯铅组、空白对照组。将对数生长期的L-929细胞用胰蛋白酶消化后制备成浓度为1×107L-1的细胞悬液,接种于培养板,待细胞贴壁生长后弃去原培养液。磷酸盐缓冲液冲洗后分别加入4种金属材料的浸提液和空白对照组的DMEM培养液。培养1,3,5,7d在倒置相差显微镜下观察细胞形态。每孔加入MTT后继续培养,抽出浸提液,磷酸盐缓冲液冲洗后加入二甲基亚砜,在490nm波长下用酶联免疫检测仪上测定吸光度值(A值),计算细胞相对增殖率,相对增殖率=(实验组A值/空白对照组A值)×100%。相对增殖率为≥100%,80% ̄99%,50% ̄79%,30% ̄49%,0 ̄29%时细胞毒性分别为0,1,2,3,4级。②急性溶血性试验:抽取新西兰兔血10mL,制成新鲜抗凝稀释兔血。将Ti-6Al-7Nb合金、Ti-6Al-...

目的:评价Ti-6Al-7Nb合金的细胞相容性和组织相容性。方法:实验于2006-05/10在解放军第四军医大学口腔生物学实验室完成。实验分组:①细胞毒性试验:按照GB/T16886.5-2003《医疗器械生物学评价》体外细胞毒性的试验方法进行。分为5组:Ti-6Al-7Nb合金组、Ti-6Al-4V合金组、纯钛组、纯铅组、空白对照组。将对数生长期的L-929细胞用胰蛋白酶消化后制备成浓度为1×107L-1的细胞悬液,接种于培养板,待细胞贴壁生长后弃去原培养液。磷酸盐缓冲液冲洗后分别加入4种金属材料的浸提液和空白对照组的DMEM培养液。培养1,3,5,7d在倒置相差显微镜下观察细胞形态。每孔加入MTT后继续培养,抽出浸提液,磷酸盐缓冲液冲洗后加入二甲基亚砜,在490nm波长下用酶联免疫检测仪上测定吸光度值(A值),计算细胞相对增殖率,相对增殖率=(实验组A值/空白对照组A值)×100%。相对增殖率为≥100%,80% ̄99%,50% ̄79%,30% ̄49%,0 ̄29%时细胞毒性分别为0,1,2,3,4级。②急性溶血性试验:抽取新西兰兔血10mL,制成新鲜抗凝稀释兔血。将Ti-6Al-7Nb合金、Ti-6Al-4V合金各5g浸泡入10mL生理盐水的试管。阳性对照组及阴性对照组分别为10mL的蒸馏水及生理盐水。每个试管中加0.2mL稀释兔血,离心后取上清液,用分光光度计在波长为545nm波长下测吸光度值,计算溶血率,溶血率=(Dt-Dnc)/(Dpc-Dnc)×100%。Dt,Dnc,Dpc分别代表Ti-6Al-7Nb合金组或Ti-6Al-4V合金组、阴性对照组、阳性对照组的A值。③短期皮下埋植试验:在10只新西兰大白兔皮下至肌肉间制备皮囊植入材料。囊底至切口>10mm,两皮囊间相隔>10mm,使植入材料相互之间不接触。每只兔植入4种金属材料各1个。于术后1,4,12周麻醉后处死动物各3只,取材料周围组织行苏木精-伊红染色,观察炎性细胞数量及种类,使用电子测量尺Version1.0测量纤维包膜厚度。参照GB/T16175-1996中的评价标准确定组织反应程度,其反应程度主要通过试验区组织中炎性细胞数量及种类变化,纤维包膜形成与否及厚度变化评价。结果:①细胞毒性评价:3种钛金属材料浸泡7d时L-929细胞形态正常,生长近汇合,排列密集规则;纯铅组可见大量细胞死亡。浸泡7d时Ti-6Al-7Nb、Ti-6Al-4V、纯钛、纯铅相对增殖率分别为100%,97%,101%,9%。经过评价,Ti-6Al-7Nb合金和纯钛的细胞毒性为0级,Ti-6Al-4V合金为1级,纯铅为4级。②细胞相容性评价:Ti-6Al-7Nb合金组、Ti-6Al-4V合金组溶血率分别0.95%,1.08%,低于国家标准规定的5%界限,无明显的急性溶血性。③组织相容性评价:肉眼可见材料被纤维包膜包裹,3组钛金属周围包膜透明且较薄,纯铅组包膜较厚呈半透明乳白色。术后12周时3种钛金属材料周围炎性细胞密度较低,包膜致密且进一步变薄。纯铅组炎性细胞数量及包膜厚度均较3种钛金属为高,组织反应较重。Ti-6Al-7Nb合金和纯钛炎性细胞/组织反应程度均为Ⅰ级,Ti-6Al-4V合金分别为Ⅰ、Ⅱ级,纯铅分别为Ⅱ、Ⅲ级。结论:Ti-6Al-7Nb合金具有良好的细胞相容性和组织相容性,是一种理想的生物医用钛合金。

 
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