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  iodine
    Effect of Iodine and T_3 on~3H—Leuoine and~3H—Tdr uptake of brain cell from chick embryos in early cultivation in vitro
    、T_3对培养早期的鸡胚脑细胞摄取~3H—亮氨酸和~3H—TdR的影响
短句来源
    Effects of inborn iodine deficiency on T_3 T_4 nuclear T_3 receptor and T_45'-deiodinase activity in neonatal rat brain
    先天性缺乏对新生大鼠脑内 T_3T_4核 T_3受体与 T_45′-脱酶活性的影响
短句来源
    Effect of combined deficiency of Selenium and Iodine on myocardial thyroid hormone metabolism and α glycerophosphate dehydrogenase activity in rats
    硒缺乏对大鼠心肌甲状腺激素代谢和α-磷酸甘油脱氢酶活性的影响
短句来源
    Effects of High Dose Iodine on Brain Development in Mice
    高对小鼠脑发育的影响
短句来源
    Methods: HUVECs were subjected to iodine in various concentrations(0.5,1.0,2.0,3.0,4.0,5.0,6.0,and 8.0?μmol/L)for 24 hours,and the morphology of the cultured endothelium was observed. MTT colorimetry was used to determine the proliferation activities of the cultured endothelium.
    方法:体外培养人脐静脉血管内皮细胞株HUECV-304,在培养液中加入不同浓度的化钾,共分9组,分别为正常对照组及加组(0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0μmol/L),连续培养24h后,观察细胞形态改变并细胞计数,MTT比色法检测细胞活性。
短句来源
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  iodide
    The propidium iodide label- ing technique was applied to assay the effect of different doses of SP600125 on change of T lymphocyte cell-cycle stimulated by Con A or by phorbol ester(PDB)plus ionomycin(Ion).
    采用化丙锭染色分析不同剂量SP600125对Con A或佛波醇酯(PDB)加离子霉素(Ion)刺激的小鼠T细胞周期变化的作用。
短句来源
    Cloning and Nucleotide Sequencing of the Sodium/Iodide Symporter Gene of Human Thyroid Follicular Cell
    钠/同向转运体基因获取及其克隆与测序
短句来源
    Study of optimized reaction condition for detection of mitochondrial transmembrane staining with rhodamine 123 and propidium iodide
    罗丹明123和化丙锭测定线粒体跨膜电位反应条件优化的研究
短句来源
    Experimental Study on Effects of Potassium Iodate and Potassium Iodide in Different Dosage on Male Reproductive System of Iodine Deficiency Rat
    低大鼠补充不同剂量酸钾和化钾对雄性生殖系统影响的实验研究
短句来源
    cDNA Cloning and Expression of Rat Sodium/Iodide Symporter (rNIS) in E.coli
    大鼠钠/同向转运体(rNIS)cDNA克隆和在大肠杆菌的表达
短句来源
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  iodin e
    Effect of Iodine and T_3 on~3H—Leuoine and~3H—Tdr uptake of brain cell from chick embryos in early cultivation in vitro
    、T_3对培养早期的鸡胚脑细胞摄取~3H—亮氨酸和~3H—TdR的影响
短句来源
    Effects of inborn iodine deficiency on T_3 T_4 nuclear T_3 receptor and T_45'-deiodinase activity in neonatal rat brain
    先天性缺乏对新生大鼠脑内 T_3T_4核 T_3受体与 T_45′-脱酶活性的影响
短句来源
    Effect of combined deficiency of Selenium and Iodine on myocardial thyroid hormone metabolism and α glycerophosphate dehydrogenase activity in rats
    硒缺乏对大鼠心肌甲状腺激素代谢和α-磷酸甘油脱氢酶活性的影响
短句来源
    Effects of High Dose Iodine on Brain Development in Mice
    高对小鼠脑发育的影响
短句来源
    Methods: HUVECs were subjected to iodine in various concentrations(0.5,1.0,2.0,3.0,4.0,5.0,6.0,and 8.0?μmol/L)for 24 hours,and the morphology of the cultured endothelium was observed. MTT colorimetry was used to determine the proliferation activities of the cultured endothelium.
    方法:体外培养人脐静脉血管内皮细胞株HUECV-304,在培养液中加入不同浓度的化钾,共分9组,分别为正常对照组及加组(0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0μmol/L),连续培养24h后,观察细胞形态改变并细胞计数,MTT比色法检测细胞活性。
短句来源
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  “碘”译为未确定词的双语例句
    ~(125)I-LABELLED MONOCLONAL ANTIBODY IN THE DETECTION OF LEPTOSPIRAL ANTIGENS IN TISSUES OF GUINEA PIG
    ~(125)标记单克隆抗体检测钩端螺旋体抗原在豚鼠体内的分布
短句来源
    STUDY ON THE STABILITY OF RADIOIODINE-LABELED PORCINE ZONA PELLUCIDA ANTIGEN
    标记猪卵透明带抗原的稳定性研究
短句来源
    The study on the dependence of IgG concentration on the iodide-selective electrode potential change
    IgG含量对电极电极电位改变依赖关系的研究
短句来源
    Effect of lithium on the development of iodine-deficiency goitre
    锂与缺性甲状腺肿发病关系的实验研究
短句来源
    Reaction of 3-iodo-4-Oxo-4H-1-benzoprom-4-one with 2-thiocytosine
    3──苯并吡喃─4─酮与2─硫代胞嘧啶的反应
短句来源
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  iodine
Characterization of iodine species in the marine aerosol: To understand their roles in particle formation processes
      
In this contribution, iodine chemistry in the Marine Boundary Layer (MBL) is introduced.
      
A series of methodologies for the measurements of iodine species in the gas and particle phases of the coastal atmosphere has been developed.
      
Iodine species in the gas phase in real air samples has been determined in two field campaigns at the west coast of Ireland, indicating that gaseous iodo-hydrocarbons and elemental iodine are the precursors of new particle formation.
      
Particulate iodine speciation from the same measurement campaigns show that the non-water-soluble iodine compounds are the main iodine species during the marine particle formation.
      
更多          
  iodide
Mercury iodide and nitrate contribute more to inhibiting cathepsin B and calpains activities in the above tissues, respectively.
      
The reduction of copper iodide occurs through the solution with the formation of Cu nanoparticles, the sizes of which are comparable to those of the initial iodide particles, and is limited only by the CuI dissolution rate.
      
Computer Simulation of the Initial Stage of Water Vapor Nucleation on a Silver Iodide Crystal Surface: 1.
      
The nucleation of water vapors on the surface of a fragment of silver iodide crystal is simulated by the Monte Carlo method under the conditions similar to natural conditions in a humid atmosphere.
      
Computer Simulation of the Initial Stage of the Nucleation of Water Vapors on the Silver Iodide Crystal Surface: 2.
      
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  iodin e
The possibility of iodine-induced hyperthy roidism was not considered because there was no known previous io dine administration.
      


In this paper, we report for thefirst time the mediation of cytotoxicimmune reactions against human livercancer cells in vitro by xenogeneic "im-mune" RNA. The human liver cancercell strain (BEL-7402) employed wasestablished in tissue culture in ourlaboratory by explant from a surgicalspecimen. The cell-mediated assayusing ~(125)I-iododeoxyuridine (~(125)IUdR)-labeled target cells was used for thestudies. Normal, nonimmune humanperipheral blood lymphocytes were con-verted into "killer cells", which effectedimmune...

In this paper, we report for thefirst time the mediation of cytotoxicimmune reactions against human livercancer cells in vitro by xenogeneic "im-mune" RNA. The human liver cancercell strain (BEL-7402) employed wasestablished in tissue culture in ourlaboratory by explant from a surgicalspecimen. The cell-mediated assayusing ~(125)I-iododeoxyuridine (~(125)IUdR)-labeled target cells was used for thestudies. Normal, nonimmune humanperipheral blood lymphocytes were con-verted into "killer cells", which effectedimmune cytolysis of human liver cancercells, by incubation with "immune"RNA extracted from the lymph nodesand spleens of sheep which had pre-viously been immunized with the hu-man liver cancer cells being studied.It was observed that the cytotoxicityindex of lymphocytes increased signi-ficantly after incubation with "immune"RNA in ten experiments. The controlRNA from sheep non-specifically im-munized with complete Freund's adju-vent did not enhance cytotoxicity indexof lymphocytes except in one experi-ment. The immune response mediatedby the "immune" RNA was abrogatedwhen the "immune" RNA was treatedwith RNase prior to incubation withlymphocytes. Treatment with DNaseor pronase did not effect the activityof the "immune" RNA. From thesestudies, it was demonstrated that thexenogeneic "immune" RNA couldtransfer the cell-mediated immunitydirected against the surface antigenson the human liver cancer cells tonormal human lymphocytes. Thus, itwould be suggested that xenogeneic"immune" RNA might be consideredas an approach to immunotherapy forhuman hepatocarcinoma.

从人肝癌细胞(BEL-7402)高度免疫过的绵羊的淋巴器官中抽提的肝癌“免疫”RNA,和正常人外周血淋巴细胞温育后,在体外能介导肝癌细胞的免疫细胞溶解。用5-~(125)-2′-脱氧尿嘧啶核苷标记人肝癌靶细胞的微量细胞毒性试验进行测定时观察到,正常人淋巴细胞经肝癌“免疫”RNA处理后,其细胞毒性指数在16次实验中有10次有显著增高,但是,在与对照RNA温育后其细胞毒性指数(除一次实验外)均未出现有显著意义的变化。肝癌“免疫”RNA在用RNA酶降解后,传递细胞毒性免疫反应的活性即消失,而用DNA酶或灰链霉菌蛋白酶处理,却仍保持这种传递能力。由此证明,异种“免疫”RNA能够把针对人肝癌细胞表面抗原的细胞免疫性传递给正常人淋巴细胞。

AEPs were recorded at 1046 paramedian midbraih sites in 31 cats of both sexes when electrical stimulations were applied to 46 points within the HVMs after the cats had been anesthetized with nembutal and paralyzed with flaxedil. In the 103 responsive sites where AEPs were recorded, 151 fast wave responses were obtained. When the HVM was stimulated with electrical pulses of increasing intensity, two types of midbrain responses were recorded:"graded" and "all or none". Responsiveness of AEPs was tested for using...

AEPs were recorded at 1046 paramedian midbraih sites in 31 cats of both sexes when electrical stimulations were applied to 46 points within the HVMs after the cats had been anesthetized with nembutal and paralyzed with flaxedil. In the 103 responsive sites where AEPs were recorded, 151 fast wave responses were obtained. When the HVM was stimulated with electrical pulses of increasing intensity, two types of midbrain responses were recorded:"graded" and "all or none". Responsiveness of AEPs was tested for using paired stimui (PST) of different frequencies. Of the 111 AEPs tested, 38 could follow PST above 500Hz, 27 between 200-499Hz, and 46 under 199Hz. In order to test the responsiveness of AEPs further, short trains of pulses were applied. The results suggested that there might be both transsynaptic and direct connections between the two brain regions. According to calculations,the conduction velocity over this path was about 0. 29-4. 1m/sec., similar to the conduction velocity in thin myelinated and nonmyelinated fibers of the peripheral nerve. On the basis of the electrophysiological and histological data, it is suggested that there might be a topographical organization between HVM and mesencephalic central gray in dorsal-dorsal ventral-ventral direction. Some characteristics of responses which may belong to central axons are described. Advantages of the method of combining PST test with short train test in the evaluation of responsveness of the neural tissues are discussed..

用电脉冲刺激戊巴比妥麻醉或三季铵酚麻醉的31只猫的HVM,记录了旁中央中脑区的AEP。HVM刺激点46个,旁中央中脑区引导点1046个,得阳性反应点103个,锋电位式反应151个。用逐渐增强的电脉刺激HVM可得“等级”式和“全或无”式两类反应。对111个反应用不同频率的双刺激测试其反应性,其中有38个能跟随500Hz以上的PST;27个能跟随200~499Hz的PST;46个只能跟随199Hz以下的PST。给予短串刺激时,得到跟随500Hz以上和500Hz以下的两类反应。这些结果提示HVM与旁中央中脑区既有经突触的投射,又有直接的纤维连接。据计算,这些直接纤维的传导速度约为0.29~4.1m/秒,相当于细有髓鞘和无髓鞘两类纤维。描述了可能属于中枢轴突的某些传导特性。反映直接纤维连接的HVM刺激点与中脑中央灰质反应点的位置关系提示两者之间可能具有背腹方向的对应关系。讨论了双刺激与短串刺激方法结合应用对于评价所研究神经组织反应特性的某些优点。

HRP has been conjugated to anti-HBs (goat IgG) through the two-step glutaralde-hyde coupling, benzoquinone coupling, or modified periiodate coupling methods. The characterization of conjugates were determined by agar diffusion and 3,3'-diaminobenzidine staining process. Besides, we have detected the concentration and activity of HEP, the concentration of sheep IgG and its immunoactivity on anti-HBs and have explained the meaning pf labelling rate (A403nm/A280nm). According to ELISA assay, we proposed that the...

HRP has been conjugated to anti-HBs (goat IgG) through the two-step glutaralde-hyde coupling, benzoquinone coupling, or modified periiodate coupling methods. The characterization of conjugates were determined by agar diffusion and 3,3'-diaminobenzidine staining process. Besides, we have detected the concentration and activity of HEP, the concentration of sheep IgG and its immunoactivity on anti-HBs and have explained the meaning pf labelling rate (A403nm/A280nm). According to ELISA assay, we proposed that the labelling rate might be used as a criterion for evaluating the quality of HBP conjugates and with the advantage of convenience for calculating.

辣根过氧化物酶(HRP)和抗HBs(山羊IgG)按戊二醛二步法、苯醌法和改良过酸盐法交联反应分别制成酶结合物,用琼脂扩散、二氨基联苯胺染色法对酶结合物定性,并测定其HRP浓度和活性、羊IgG浓度和抗HBs免疫活性,阐明标记率(A403nm/A280nm)的含义,通过ELISA检测表明标记率可作为评价酶结合物质量的指标,且其计算简便。

 
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