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| | sing highly purified complement C 1q component extracted from fresh pig sera,we detected hu-man C1q binding circulating immune complex (CIC) by ELISA system. We found concentrations of CIC in normal control were less than 3 3mg/L, AHG but markedly elevated in the acute phase of acuts glomerulonephritis and Kawasaki disease and gradually returned to normal during remission. It indicates that this method is very sensitive and reliable. The advantages of pig sera C1q compared to human sera C1q are less expensi... | | 为更好测定人血清循环免疫复合物(CIC),从新鲜猪血中据取高纯度的猪血补体成分C1q,采用酶联免疫吸附技术建立了猪血C1q测定CIC的方法。人血清CIC测定结果用热凝集免疫球蛋白(AHG)表示,正常人群血清CIC<33mg/LAHG。测定37例急性肾小球肾炎和14例川崎病患者,其血清CIC浓度在发病急性期普遍升高,经过治疗缓解或病愈后,CIC浓度逐步降为正常水平。该法所用的猪血来源丰富,可避免乙型肝炎、艾滋病源的污染,降低了实验背景的测定值,使结果更接近于真实水平,有助于临床对免疫复合物性疾病的诊断及疗效判断。 | | 文摘来源 | | This paper studied by alkali dissolution and enzyme hydrolysis to extract protein of sesame dregs.Firstly,the optimum processing of Alkali Dissolution extracting protein was determined;then these factors of pH,temperature,enzyme content,time that effect the extraction ratio of enzyme hydrolysis was studied,and uthe optimum level of every factor was determined,then the best condition of enzyme hydrolysis were determined by orthogonal experiments.The results showed that the optimum processing of alkali dissol... | | 本文采用碱酶两步法提取麻渣中的蛋白质,首先确定了碱溶法最佳工艺;然后对影响酶解法提取率的四个因素pH值、温度、酶用量、时间分别做了单因素试验,确定了各因素的最佳水平,再通过正交实验确定了酶解法的最佳工艺条件。结果表明:碱溶法最佳工艺为固液比1:20,pH值为10,时间3h,温度50℃;酶解法的最佳工艺条件为pH值11,酶用量150U/mL,酶解温度40℃,时间4h。碱酶两步法提取使麻渣蛋白质提取率达到81.21%。本文还对所提麻渣蛋白的吸水性、湿润性、吸油性、热凝集性和粘度等功能特性进行了研究,并与大豆分离蛋白、酪蛋白和芝麻蛋白的相关性质进行比较。结果表明:麻渣蛋白具有较好吸水性、持水力、湿润性;较弱的吸油性;pH值对热凝集性和粘度的影响较大。 | | 文摘来源 | |   |
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