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   转录泡 的翻译结果: 查询用时:0.375秒
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转录泡
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     About Opera
     歌剧
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     Studies on RNA Transcription Activity in Interphase Nuclei in Physarum polycephalum
     多头绒菌间期细胞核中RNA的转录状况(英文)
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     About Opera
     次歌剧
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     Advances in the FoxO Class of Transcription Factors
     FoxO转录因子
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     2. In vitro transcription;
     (2)体外转录
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  transcription bubble
A byproduct of transcription bubble mapping with permanganate was the ability to detect TBP protection of the TATA sequences.
      
Moreover, density of within the transcription bubble would enable similar size and position was seen in the X-ray structure in the channel.
      
These have the appearance of the transcription bubble seen with paused Pol II on Drosophila heat shock genes.
      
The presence of a paused pol II should result in a transcription bubble that contains a region of unpaired DNA.
      
We next added the nontemplate strand and the upstream double-stranded DNA to the model to complete the transcription bubble.
      


Characterization of the processivity determinants in eukaryotic RNA polymeraseⅡ is crucial for understanding both the mechanisms of eukaryotic gene expression at the level of promoter escape, pausing, release of the RNA from transcription terminator regions, and the mechanisms of transcription-coupled repair of DNA damage (TCR). The sliding clamp model of transcription processivity suggests the formation of a highly stable elongation complex (EC), in which RNA polymerase is tightly bound to the nascent transcript...

Characterization of the processivity determinants in eukaryotic RNA polymeraseⅡ is crucial for understanding both the mechanisms of eukaryotic gene expression at the level of promoter escape, pausing, release of the RNA from transcription terminator regions, and the mechanisms of transcription-coupled repair of DNA damage (TCR). The sliding clamp model of transcription processivity suggests the formation of a highly stable elongation complex (EC), in which RNA polymerase is tightly bound to the nascent transcript and template forming the characteristic transcription "bubble". Here, an in vitro system for promoter-independent assembly of a functional mammalian RNA polymeraseⅡ elongation complex using highly purified polymerases and synthetic RNA and DNA oligonucleotides was presented. It was shown that the 9-nucleotide RNA∶DNA template hybrid is necessary and sufficient for the formation of a stable elongation complex with human RNA polymeraseⅡ, while further inclusion of the non-template DNA strand to form the complete transcription "bubble" actually destabilizes the already formed RNA:DNA:RNA polymeraseⅡ complex. In addition, by introducing a DNA damage at a specific site in the template DNA, this assay can potentially be used for characterizing the processes of transcription-coupled repair of DNA damages.

真核生物RNA聚合酶Ⅱ的持续合成能力对基因转录过程中每一个阶段,包括启动子脱离、转录暂停、转录终止以及转录偶联DNA损伤修复过程的调节至关重要.在RNA聚合酶Ⅱ介导的转录延伸过程中,其和模板DNA及转录产物RNA紧密结合,形成一个非常稳定的延伸三维复合物(elongationcomplex,EC).此特征性“泡”状结构的形成是RNA聚合酶Ⅱ持续合成能力所必需的.在不依赖启动子及众多转录起始因子的条件下,利用人工合成的RNA与DNA寡核苷酸,在体外组装形成具有功能转录活性的延伸复合物.结果表明,长度为9个核苷酸的RNA与模板DNA形成的杂合分子对转录延伸复合物的形成是必需的,而非转录模板DNA链的加入导致最终活性转录“泡”状复合物的形成,并可转录形成与模板相关的转录产物,进一步通过在模板DNA的特定位置引入一个乙酰氧乙酰氨基芴修饰基团,可特异性地阻断转录延伸过程,从而显示该系统在研究真核基因转录及转录偶联DNA损伤修复机制中的潜在应用价值.

 
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