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Tris碱
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     Basic knowledge of analytical reagents——alkalis
     二、
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     PCR reaction system, Taq enzyme, primer et al.
     PCR反应体系、Taq酶、引物,Tris及聚丙烯酰胺等;
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     About Alkali Saving
     节工程
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     Methods CA activity in unbalanced IMCD cells was measured with imidazole Tris technique.
     方法 采用咪唑 Tris技术测定呼吸性酸失衡IMCD细胞的CA活性 ;
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  tris base
Unbound dye is removed by a series of four 2.5 min washes in 1% acetic acid, and protein-bound dye extracted with 10 mM unbuffered Tris base for spectrophotometric optical density determination at 433 nm.
      
A stock peptide solution was prepared and adjusted to pH 7.4 with Tris base.
      
Antibody was eluted in 100 mM glycine, pH 2.4, and the eluate was neutralized with Tris base.
      
Bound antibodies were eluted with 0.1 M glycineHCl, pH 2.8, and were immediately neutralized by the addition of 1 M Tris base.
      
Control solution was titrated to pH 7.4 with NaOH and Na-free solution with Tris base.
      
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At 37 ℃ and in Tris-HCl buffer (pH=7.4), enthalpy magnification technique and thermoki- netic initial rate method were employed to study the hydrolysis reaction of acetylcholine bromide catalyzed by acetylcholinesterase (AchE) and the inhibition of the reaction by sodium dodecyl sulfate (SDS) under near physiological conditions. Molar enthalpy (?Hm,1) of the enzymatic reaction was determined to be 0.63 kJ?mol-1 by deducting enthalpy of protonization of Tris base from total enthalpy of reaction system. Micha-...

At 37 ℃ and in Tris-HCl buffer (pH=7.4), enthalpy magnification technique and thermoki- netic initial rate method were employed to study the hydrolysis reaction of acetylcholine bromide catalyzed by acetylcholinesterase (AchE) and the inhibition of the reaction by sodium dodecyl sulfate (SDS) under near physiological conditions. Molar enthalpy (?Hm,1) of the enzymatic reaction was determined to be 0.63 kJ?mol-1 by deducting enthalpy of protonization of Tris base from total enthalpy of reaction system. Micha- elis constant (Km) and substrate inhibition constant (KS) were also determined to be 0.85~0.94 mmol?L-1 and 0.74~0.83 mmol?L-1, respectively. SDS could remarkably decrease the rate of enzyme reaction but has less influence on biochemical constants of the enzyme. In SDS solution, inactivation rate of AchE follows first order reaction kinetics, the apparent first order inactivation constant is linear to action time and the fourth power of SDS concentration, and inactivation constant was calculated to be (2.47~2.69)×1013 mol-4?L4?min-1.

在37℃,pH=7.4的Tris-HCl缓冲体系中,利用热焓放大技术和热动力学初始速率法研究了近生理条件下的乙酰胆碱酯酶(AchE)催化溴化乙酰胆碱水解反应及十二烷基硫酸钠(SDS)对反应的抑制动力学.通过测量实验条件下反应体系的总反应焓及相同条件下的Tris碱的质子化焓,确定了酶反应的摩尔反应焓ΔHm,1为0.63kJ?mol-1,米氏常数Km和底物抑制常数KS分别为0.85~0.94mmol?L-1和0.74~0.83mmol?L-1.SDS能够显著地降低反应速率,但对酶反应的生化常数的影响较小,SDS对AchE的抑制表现为不可逆抑制.在一定浓度的SDS溶液中,AchE的失活符合一级反应动力学规律,表观一级失活速率常数与作用时间及SDS浓度的四次方呈线性关系,失活常数为(2.47~2.69)×1013mol-4?L4?min-1.

 
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