Objective: To further investigate the action mechanisms of berberine (Ber) and to assess the effects of Ber on the mRNA expression of phosphodiesterase type 5 (PDE5) in rat corpus cavernosum.
PDE activity was assayed by using 3H-cGMP as substrate, the PDE isoenzymes hydrolyzed it to 3H-GMP, and 3H-GMP was further hydrolyzed to 3H-guanosine by 5′-nuclease of snake venom. Add scintillation cocktail to observe the PDE isoenzymes activity.
方法以牛阴茎海绵体为原料 ,通过快速蛋白液相系统分离纯化得到PDE同工酶 ,然后以3 H cGMP为底物 ,经纯化的PDE同工酶催化水解为3 H GMP ,进一步在含 5′ 核苷酸酶的蛇毒液作用下 ,脱去 5′ 磷酸生成3 H 鸟苷 ,在闪烁剂激发下测定3 H 鸟苷的变化 ,绘制PDE活性曲线。
CONCLUSION Sildenafil increases cGMP concentration in RCC is attributed to the augmentation of NO/cGMP pathway, and the accumulation of cGMP improves the relaxation of RCC.
Needle biopsy of the corpus cavernosum with the Biopty gun system has been performed in impotent patients for obtaining erectile tissue to identify the presence of intracavernous structures (smooth muscle cells, collagen, arteries, and nerves).
Moreover, there is a potential risk of damage to the corpus cavernosum.
Penile fracture (PF) is an uncommon injury defined as the rupture of the tunica albuginea and corpus cavernosum of the penis, usually resulting from direct blunt trauma to the erect penis during sexual intercourse or masturbation.
Clearly, novel molecular targets in the corpus cavernosum smooth muscle (CCSM) need to be identified and evaluated for the potential in development of additional oral agents or gene therapy for ED.
Peyronie's disease is an idiopathic, localized connective tissue disorder of the penis that involves the tunica albuginea of the corpus cavernosum and the adjacent areolar space.
The availability of such cultures should facilitate the study of endothelium-mediated responses which play an important role in the erectile function of human penile corpus cavernosum.
Between Jan. and Dec.1994,self-injection of aloprostadil sterile powder(prostaglandin E_1,caverject)for erectile dysfunction(ED)has been studied in 60 patients. Optimal response has been achieved in 61 %. 26.7% of patients experienced some mild pain after the injection. No penile hematoma,priapism or cavemous fibrosis has been Observed.Intracavernous injection of aloprostadil sterile powder is believed to be safe and effective for ED of various origin with relatively low side effects.
Effect of 5 kinds of α-adrenoceptor antagonist on contractile response of the rabbit penile corpus cavernosum was studied by using functional study. The results showed that the competitive effect of prazosin was the strongest among the five agents, followed by tamsulosin. No significant effect was found with phentolamine, terazosin and yohimbine. The results provide experiment basis for selecting and appling α-adrenoceptor antagonists in the treatment of penile erectile dysfunction.
Sildenafil is the first oral therapeutic agent for erectile dysfunction. Sildenafil is a selective inhibitor of cGMP specific phosphodiesterase (PDE 5). Penile erection involves relaxation of the corpus cavernosum, an event mediated by NO and cGMP. The biological actions of cGMP are terminated by phosphodiesterase enzymes and PDE 5 is the major cGMP metabolising enzyme in this tissue. Sildenafil is relatively safe compared to erection injectables because it does not relax on isolated human corpus cavernosum,...
Sildenafil is the first oral therapeutic agent for erectile dysfunction. Sildenafil is a selective inhibitor of cGMP specific phosphodiesterase (PDE 5). Penile erection involves relaxation of the corpus cavernosum, an event mediated by NO and cGMP. The biological actions of cGMP are terminated by phosphodiesterase enzymes and PDE 5 is the major cGMP metabolising enzyme in this tissue. Sildenafil is relatively safe compared to erection injectables because it does not relax on isolated human corpus cavernosum, and does not cause priapism. Due to the tendency of abuse of sildenafil, its adverse cardiovascular associations with myocardial infaraction, ventricular arrhythmia and hypertension need to be alerted.$Key words sildenafil; sexual dysfunction; phosphodiesterase enzyme 5 was no remarkable difference between the two methods ( P >0 05) CONCLUSION: The sugarcoat had no effect on the determination of verapamil hydrochloride tablets
阴茎海绵体
corpus cavernosum
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