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自身溶血
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  autohemolysis
     Morphology changes were observed by optic and electron microcope and membrane fragility, autohemolysis, cholesterol, methemoglobin, acetylcholin activity, ATP, 2,3-DPG and P50 were detected for evaluating membrane integrality, metamorphosis, oxygen, and so on.
     ③光镜和扫描电镜观察酶解前后猕猴红细胞的形态,检测其变形性、细胞膜脆性、自身溶血率、胆固醇、高铁血红蛋白、乙酰胆碱脂酶、ATP、2,3-DPG、P_(50)等红细胞的结构功能指标,了解酶解红细胞的膜完整性、变形能力、携氧能力以及代谢能力。
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  “自身溶血”译为未确定词的双语例句
     mPEG modification didn't effect the RBCs function and structure, including osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3-DPG, deformability and morphology.
     mPEG修饰没有影响红细胞的结构功能,包括渗透脆性、自身溶血、乙酰胆碱酯酶活性、膜胆固醇含量、ATP、2,3-DPG、红细胞变形性以及红细胞形态等;
短句来源
     It could camouflage RhD antigen efficiently when the concentration reached to 1 mmol/L. The result also showed that there were no harmful effects of mPEG modification on cell morphology, osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3 DPG and deformability.
     结果表明 ,mPEG BTC(苯并三唑端基PEG)较其他 3种PEG的修饰效果好 ,在 1mmol/L时可以有效地遮蔽红细胞表面RhD抗原 ,而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2 ,3 DPG含量以及变形性无影响。
短句来源
     Results: The antigenic activity of Jk a, Jk b, k and P 1 on RBC surface disappeared after modification by mPEG-BTC at the concentration of 1.0 mmol/L. There were no harmful effects on cell morphology,structure and function including osmotic fragility,hemolysis,AchE,cholesterol, ATP, 2,3-DPG and deformability. The other two kinds of mPEG could not achieve such camouflage effect.
     结果 :实验表明 ,在 1.0mmol/L时mPEG BTC(苯并三唑碳酸酯端基PEG)就可以使红细胞表面Jka,Jkb,k和P1抗原性消失 ,而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2 ,3 DPG含量、变形性以及存活期无影响 ,其他 2种mPEG对红细胞修饰效果不好。
短句来源
     Method The five macacas, two with group A and three with group B, were selected as subjects for their stronger anthropoid human ABO antigen from 30 macacas by the heat absorption-elution test. The anthropoid human B antigens of the macacas were treated by the enzymolysis of gene-recombined α-galactosidase in vitro and the enzyme-treated cells were transfused into monkeys.
     方法 采用热吸收放散试验从 30只华南猕猴中选取类人ABO血型抗原较强的 2只A型、3只B型猕猴做为实验对象 ,以基因重组的α 半乳糖苷酶体外酶解猕猴类人B型血抗原 ,并回输到A型猕猴体内 ,测定红细胞脆性、自身溶血率、胆固醇、高铁血红蛋白、乙酰胆碱脂酶、ATP等红细胞的结构功能指标。
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  相似匹配句对
     hemolytic test;
     溶血试验;
短句来源
     AUTOLOGOUS MIXED LYmPHOCYTE REACTION
     自身混合淋巴细胞反应
短句来源
     Hemolytic activity of swine peripheral mononuclear cells to autologous RBC
     猪单个核细胞对自身红细胞溶血活性的影响
短句来源
     Analysis of 23 patients with child hemolytic uremia syndrome
     小儿溶血尿毒症
短句来源
     (3)self condensation;
     (3)自身凝集;
短句来源
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  autohemolysis
The autohemolysis of the erythrocytes was diminished with dependence upon the doses until to 59.3% of the control value.
      
Therefore, only cytotoxic effects of uremic serum on the lymphocytes may be due to higher molecular urine metabolites retained in uremia whereas increased autohemolysis will be induced by toxins of lower molecular weight.
      
The cells were stomatocytic with increased osmotic fragility and autohemolysis.
      
Red cell survival in chronic uraemia: Its relationship with the spontaneous in vitro autohemolysis and with the degree of anaemi
      
Erythrocytes' shows increased osmotic fragility with autohemolysis in hypotonic solutions.
      
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Rh is a very important blood group like ABO blood system in transfusion medicine. It causes severe transfusion reaction and hemolytic disease of the newborn (HDN) if RhD blood group does not match between the donor and the recipient. The population of RhD negative is only about 0.2%-0.5% in Chinese. Conversion of RhD positive RBCs to RhD negative is very important in clinical transfusion. This study was to try to modify RhD antigen located on the surface of A, B,O and AB red blood cells in order to convert RhD...

Rh is a very important blood group like ABO blood system in transfusion medicine. It causes severe transfusion reaction and hemolytic disease of the newborn (HDN) if RhD blood group does not match between the donor and the recipient. The population of RhD negative is only about 0.2%-0.5% in Chinese. Conversion of RhD positive RBCs to RhD negative is very important in clinical transfusion. This study was to try to modify RhD antigen located on the surface of A, B,O and AB red blood cells in order to convert RhD positive to RhD negative by the modification of four kinds of methoxypolyethylene glycol (mPEG) derivatives and to observe the effect of mPEG modification on cell morphology, structure and function. The result demonstrated that modification efficiency of mPEG BTC (mPEG benzotriazole carbonate) was better than other three kinds of mPEG derivatives. It could camouflage RhD antigen efficiently when the concentration reached to 1 mmol/L. The result also showed that there were no harmful effects of mPEG modification on cell morphology, osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3 DPG and deformability. It is suggested that success in converting RhD positive RBCs to RhD negative was preliminarily achieved.

Rh血型与ABO血型一样是重要的血型系统 ,Rh血型不符会引起严重的输血反应及新生儿溶血病。中国人群中RhD阴性血型的比例仅为 0 .2 % - 0 .5 % ,将RhD阳性红细胞改造成RhD阴性红细胞在临床输血中有重要的意义。本研究用 4种不同端基的甲氧基聚乙二醇 (mPEG) ,修饰A型、B型、AB型和O型的RhD( + )红细胞 ,比较 4种mPEG衍生物对RhD抗原的修饰效果。同时观察mPEG修饰对红细胞形态、结构和功能的影响。结果表明 ,mPEG BTC(苯并三唑端基PEG)较其他 3种PEG的修饰效果好 ,在 1mmol/L时可以有效地遮蔽红细胞表面RhD抗原 ,而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2 ,3 DPG含量以及变形性无影响。结论 :初步实现了将RhD( + )红细胞修饰改造成RhD( - )红细胞的目的。

Objective: To study camouflage of rare blood group antigen on red blood cells with methoxy polyethylene glycol(mPEG), and to find a possible way to prepare universal donor red blood cells( RBCs). Methods: Three kinds of mPEG derivatives were used to modify human RBCs.The camouflage effects of mPEG on four kinds of rare blood group antigen located on RBC surface and the influence of mPEG modification on cell structure and function were observed. Results: The antigenic activity of Jk a, Jk b, k and P 1 on RBC...

Objective: To study camouflage of rare blood group antigen on red blood cells with methoxy polyethylene glycol(mPEG), and to find a possible way to prepare universal donor red blood cells( RBCs). Methods: Three kinds of mPEG derivatives were used to modify human RBCs.The camouflage effects of mPEG on four kinds of rare blood group antigen located on RBC surface and the influence of mPEG modification on cell structure and function were observed. Results: The antigenic activity of Jk a, Jk b, k and P 1 on RBC surface disappeared after modification by mPEG-BTC at the concentration of 1.0 mmol/L. There were no harmful effects on cell morphology,structure and function including osmotic fragility,hemolysis,AchE,cholesterol, ATP, 2,3-DPG and deformability. The other two kinds of mPEG could not achieve such camouflage effect. Conclusion: The mPEG-BTC can camouflage the rare blood group antigen of Jk a, Jk b, k and P 1 on RBC surface efficiently and has no influence on structure and function of modified RBCs.

目的 :研究用甲氧基聚乙二醇 (methoxypolyethyleneglycol,mPEG)修饰红细胞稀有血型抗原 ,为制备通用型血寻找可能的途径。方法 :用 3种具有不同端基的mPEG修饰红细胞 ,观察其对红细胞表面 4种稀有血型抗原的修饰效果及对红细胞结构、功能和存活期的影响。结果 :实验表明 ,在 1.0mmol/L时mPEG BTC(苯并三唑碳酸酯端基PEG)就可以使红细胞表面Jka,Jkb,k和P1抗原性消失 ,而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2 ,3 DPG含量、变形性以及存活期无影响 ,其他 2种mPEG对红细胞修饰效果不好。结论 :mPEG BTC可有效遮蔽红细胞表面的稀有血型抗原 ,对其结构、功能及存活期无影响 ,本实验为mPEG包裹法制备通用型血提供了有力的实验证据

Objective To observe the enzymolysis effect of α-galactosidase on anthropoid human B antigen of the macacas and to explore the impact of enzyme-treatment by α-galactosidase on the structure and function of RBC. Method The five macacas, two with group A and three with group B, were selected as subjects for their stronger anthropoid human ABO antigen from 30 macacas by the heat absorption-elution test. The anthropoid human B antigens of the macacas were treated by the enzymolysis of gene-recombined α-galactosidase...

Objective To observe the enzymolysis effect of α-galactosidase on anthropoid human B antigen of the macacas and to explore the impact of enzyme-treatment by α-galactosidase on the structure and function of RBC. Method The five macacas, two with group A and three with group B, were selected as subjects for their stronger anthropoid human ABO antigen from 30 macacas by the heat absorption-elution test. The anthropoid human B antigens of the macacas were treated by the enzymolysis of gene-recombined α-galactosidase in vitro and the enzyme-treated cells were transfused into monkeys. And erythrocyte fragility, autohemolysis rate, cholesterol, methemoglobin, acetylcholin esterase, ATP were detected in recipient blood. Results The membranes of RBC of monkeys treated by α-galactosidase were intact and oxygen carrying capacity of RBC was normal. No transfusion reaction happened after the treated “general" blood was transfused into receptor monkeys. Conclusion The enzymolysis by the gene-recombined α-galactosidase had no adverse effects on appearance, structure and function of RBC and was safe in recipient macaca's body.

目的 观察α 半乳糖苷酶对猕猴类人B抗原的酶解效果 ,探讨α 半乳糖苷酶酶解对猕猴红细胞结构、功能的影响。方法 采用热吸收放散试验从 30只华南猕猴中选取类人ABO血型抗原较强的 2只A型、3只B型猕猴做为实验对象 ,以基因重组的α 半乳糖苷酶体外酶解猕猴类人B型血抗原 ,并回输到A型猕猴体内 ,测定红细胞脆性、自身溶血率、胆固醇、高铁血红蛋白、乙酰胆碱脂酶、ATP等红细胞的结构功能指标。结果 经α 半乳糖苷酶酶解后 ,猕猴红细胞胞膜完整、携氧能力正常 ,酶解后的“通用”型血回输给受体猕猴无任何输血反应发生。结论 α 半乳糖苷酶酶解对于猕猴红细胞的形态、结构、功能无不良影响 ,且在实验动物体内是安全的。

 
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