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| | A method of using dextran-Sulfate ( D.S. ) and Catt in combination with poly-aerylamide gel preparative electrophoresis to isolate serum high density lipoprotein ( HDL ) in a pure form was developed. In this method HDL was precipitated by D.S, and Catt from serum freed previously of very low density lipoprotein (VLDL ) and low density lipoprotein ( LDL ) in a form of complex, which can be redissolved in 12% NaCl and reprecipitated by dilution and addition of D.S. and Catt. The above process was repeated twi... | | 作者以多聚阴离子—硫酸右旋糖酐沉淀分离,再以聚丙烯酰胺凝胶制备电泳进一步纯化,可获得较纯净的血清高密度脂蛋白。为不具备超速离心机的实验室提供一个可以较大量分离提纯血清高密度脂蛋白的方法。 | | 文摘来源 | | An improved microanalytioal method for determination of serum high-density lipoprotein (HDL)-cholesterol is herein presented. 80 μl of serum in a small test tube was added to sodium phosphotungstate and Mg3+ mixed reagent. This solution was drawn up into a capillary tube, which was sealed and centrifuged at room temperature for 30 minutes for precipitation of both the serum low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL). Cholesterol was then determined in the supernatant by means of i... | | 本文介绍一种改进的微量快速测定血清高密度脂蛋白胆固醇的方法。取80微升血清置小试管中,加入20微升磷钨酸钠—镁混合试剂混匀,用毛细管吸取后封口,于室温1,500×g离心半小时以沉淀血清中的低密度和极低密度脂蛋白。然后用磷苯二甲醛方法测定上层清液中的高密度脂蛋白胆固醇。方法简便、快速,重复性良好。经用本法测定285例健康成人,结果与文献报道的全量法比较,极为相近,相关系数γ=0.84。而且用血量少,无需特殊仪器设备,又可用于高甘油三酯血症的标本。不仅适于临床常规,尤适用于大规模的流行病学调查。 | | 文摘来源 | | Serum high density lipoprotein (HDL)-cholesterol levels in 2,399 healthy subjects from newborn to 78 years of age were analyzed. Factors influencing on their variations were summarized as follows: (1) The frequency distribution of HDL-cholesterol levels was approximately normal in most age groups, although a skew to the right was observed before the age of 20 years for both sexes. (2) Serum HDL-cholesterol and ratio of HDL-cholesterol/total cholesterol varied with age. The mean value ( ± SD) of HDL-choleste... | | 本文测定2,399例从新生儿至78岁的上海市健康人的血清高密度脂蛋白(HDL)-胆固醇含量,分析某些可能的影响因素,所得结果如下: 1.各年龄组的频率分布,20岁以下,不论男女HDL-胆固醇含量均呈正偏态分布,而20岁以上则多数年龄组基本上均呈正态分布。2.健康人血清HDL-胆固醇含量及其与总胆固醇的比值均随着年龄而发生变化。30岁以上男女的HDL-胆固醇平均值±标准差分别为58.7±11.2和62.5±12.2毫克/100毫升,女性显著高于男性(P<0.01)。3.HDL-胆固醇含量随体重的增加而减低,它与体重/身高(W/H)指数呈显著的负相关。4.本研究中吸烟和饮酒习惯对HDL-胆固醇的影响尚无统计学意义。本研究中某些结果与西方国家不同,可能与环境因素有关。 | | 文摘来源 | |   | | << 更多相关文摘 |
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