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基因负荷
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  “基因负荷”译为未确定词的双语例句
     【Conclusions】 Anemia and ID are rare,but thalassemia and G6PDD are very common in this population,that is to say,there is a heavy genetic burden in the preschool children in the urban area of Zhuhai city.
     【结论】贫血和ID在珠海市3~6岁儿童中并不多见,但地贫和G6PDD则很常见,即该市儿童中有很高的遗传病基因负荷
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     4 new genes were obtained.
     U基因
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     On Segregational Load
     论单基因的分离负荷
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     Accelerated Genetic Algorithm Used to Load Forecasting for Power Systems
     电力系统负荷预测的加速基因方法
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     Overexpression of AK fbr gene in C.
     AKfbr基因在C.
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     The Method of Calculation for Heating/Cooling Load by Variable Indoor Temperature
     变温负荷计算法
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  gene load
In order to characterize the gene "load" of the IncP plasmids, restriction fragments were cloned and their DNA sequences established.
      
Figure 2 illustrates the effect of gene load on IL-6 levels and on mean IMT.
      
To examine the effect of overall gene load, a sum score for the 3 inflammatory gene haplotypes was calculated.
      
To determine whether smoking influenced the effects of gene load on IMT, interaction terms for smoking were included in the regression model.
      
Table 4 shows the odds ratios for an IMT in the highest quartile according to the number of gene load score in smokers and nonsmokers.
      


Objective To investigate the relationship between the expression of viral main genes and pathologic outcomes of infected cells in HCMV infection. Methods HCMV echelon-infected cell model was set up in vitro by coincubating passage cultured cells and HCMV with different titers. FQ-PCR was performed to evaluate the number of IE gene copies in infected cells and the expression of p52 was determined by immunohistochemical test and quantitated by computer imaging analysis. Major capsid protein (MCP) mRNA was also...

Objective To investigate the relationship between the expression of viral main genes and pathologic outcomes of infected cells in HCMV infection. Methods HCMV echelon-infected cell model was set up in vitro by coincubating passage cultured cells and HCMV with different titers. FQ-PCR was performed to evaluate the number of IE gene copies in infected cells and the expression of p52 was determined by immunohistochemical test and quantitated by computer imaging analysis. Major capsid protein (MCP) mRNA was also measured by RT-PCR. Meanwhile the cytopathologic effects(CPE) was observed by microscopy and TEM. Results Compared with group B and C in high HCMV titer, group A with low viral titer showed low IE gene load(P<0.05, P<0.01), while in group B and C, p52 expression was significantly elevated (P<0.01) and MCP mRNA was detected. At the same time, group B and C carried developing CPE and ultrastructural impairment during HCMV infection, that wasn′t detected in group A. Conclusion In HCMV infected cells, IE gene load is associated with initiation of viral genome functioning and efficient p52 expression is necessary for its complete performance. The detected MCP mRNA is an indicator of active HCMV infection.

目的 探讨人巨细胞病毒 (HCMV)主要基因序列表达情况对其感染结局的影响。方法采用不同滴度HCMV感染传代培养的人胚肺成纤维细胞 ,建立HCMV梯度感染细胞模型。分别应用FQ PCR法测定感染细胞内IE基因拷贝数、免疫组化法联合计算机病理图文分析系统检测p5 2蛋白表达水平以及RT PCR法测定MCPmRNA转录水平 ,光镜观察致细胞病变作用 (CPE)、电镜检查细胞超微结构改变。结果 IE基因在病毒滴度最低的A组细胞内负荷量最低 (P <0 .0 5 ,P <0 .0 1) ;p5 2蛋白在病毒滴度较高的B、C组表达水平明显高于A组 (P <0 .0 1) ,其表达定位于受染细胞核内 ;仅在B、C组内检测到MCPmRNA。A组未检出细胞病变 ,B、C组细胞检测到随感染时间延长而加重的CPE及超微结构改变。结论 HCMV受染细胞内IE基因负荷量与病毒基因组表达启动密切相关 ,p5 2蛋白高效表达是病毒基因组完整表达的必要条件 ,MCPmRNA转录是活动性HCMV感染的标志。

【Objective】 To investigate the prevalence of common hematological disorders in the children aged 3~6 years in the urban area of Zhuhai city. 【Methods】 1 396 children were randomly sampled among 4 653 children aged 3~6 years in the urban area.Their hematological parameters,iron status,genotypes of globin and glucoe-6-phosphate dehydrogenase(G6PD) activity were analyzed systematically by using established techniques or methods. 【Results】 The prevalence of anemia,iron deficiency(ID),G6PD deficiency(G6PDD),α-and...

【Objective】 To investigate the prevalence of common hematological disorders in the children aged 3~6 years in the urban area of Zhuhai city. 【Methods】 1 396 children were randomly sampled among 4 653 children aged 3~6 years in the urban area.Their hematological parameters,iron status,genotypes of globin and glucoe-6-phosphate dehydrogenase(G6PD) activity were analyzed systematically by using established techniques or methods. 【Results】 The prevalence of anemia,iron deficiency(ID),G6PD deficiency(G6PDD),α-and β-thalassemia were 2.72%,2.44%,3.15%,8.67% and 2.22%,respectively. 【Conclusions】 Anemia and ID are rare,but thalassemia and G6PDD are very common in this population,that is to say,there is a heavy genetic burden in the preschool children in the urban area of Zhuhai city.

【目的】了解珠海市城区3~6岁儿童常见血液病的发生率或基因携带率,为制订相应的预防、干预措施提供依据。【方法】随机从珠海市城区健康检查的4 653名3~6岁儿童中抽取1 396名作为研究对象,对其血样进行系统的血红蛋白(Hb)分析、铁代谢状态评估和珠蛋白基因分型以及6-磷酸-葡萄糖-脱氢酶缺乏症(G6PDD)的筛查。【结果】该市城区3~6岁儿童贫血、铁缺乏(iron deficiency,ID)和G6PDD发生率分别为2.72%、2.44%和3.15%;α-和β-地中海贫血(简称地贫)基因检出率各为8.67%和2.22%。【结论】贫血和ID在珠海市3~6岁儿童中并不多见,但地贫和G6PDD则很常见,即该市儿童中有很高的遗传病基因负荷

 
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