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阻遏蛋白     
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  repressor protein
     In order to construct a new negative regulation system which is regulated by tetracycline and lower toxin, the cloned Tetracycline Repressor Protein TetR of Ecoil. XL1-BLUE with Heat Shock Transcriptional Factor Athsf1a of Arabidopsis thaliana lacking fragment AtHSFC157 by the gene fusing was recombined. Then, the expression vector-pXNSC2020 to construct the Tetracycline negative regulable system of p35SC157-Om35S gus was cloned.
     为构建一新型低毒且受四环素负调控的基因表达调控系统,克隆了大肠杆菌XL1-BLUE四环素阻遏蛋白基因TetR和拟南芥热激因子基因Athsf1a缺失片段AtHSFC157,将两基因融合获得TetR:AtHSFC157融合片段作为四环素调控系统转录激活子,将其克隆入表达载体pXNSC2020中,成功构建了四环素负调控系统p35SC157-Om35Sgus。
短句来源
     Using DNA Shuffling Method to Generate Thermosensitive CI Repressor Protein of λ Phage
     利用DNA改组技术产生λ噬菌体CI温敏阻遏蛋白
短句来源
     The Kinetic Study on the Interactions of λ cI Repressor Protein with Operator
     λ噬菌体阻遏蛋白与操纵基因相互作用的动力学研究
短句来源
     The characterization of these sequences suggest that wild-type and mutant gene have the same open reading frame, which probably encode a repressor protein of 113 amino acids,and the putative promoter region and ribosome binding sites within the sequences have also been identified.
     核苷酸序列分析发现,野生型及其温度敏感型阻遏基因之间的碱基变异较大,但却存在几乎完全相同的开放读框,尤其是开放读框orfⅠ,可能编码着113个氨基酸的阻遏蛋白,并且还推定了开放读框的启动区和核糖体结合位点。
短句来源
  repressor
     Design and selection of high affinity DNA ligands for mutant single-chain derivatives of the bacteriophage 434 repressor
     噬菌体434单链阻遏蛋白的高亲和力DNA结合序列的设计和`筛选
短句来源
     The Tet repressor gene (TR) encoding the Tet repressor from pcDNA6/TR vector was incorporating into the backbone of the pVITRO3-TO.
     用相同的方法以pcDNA6/TR为模板扩增四环素阻遏蛋白基因(TR),经测序证实后插入pVITRO3-TO载体的一个多克隆位点。
短句来源
     The Tet repressor gene(TR) encoding the Tet repressor from pcDNA6/TR vector was incorporating into the backbone of the pVITRO3-TO.
     用相同的方法以pcDNA6/TR○c为模板扩增四环素阻遏蛋白基因(TR),经测序证实后插入pVITRO3-TO载体的一个多克隆位点。
短句来源
     , is a derivative of bacteriophage 434 repressor,which contains covalent1y dimerized DNA-binding domains (axnino acids 1-69) of thephage 434 repressor.
     单链阻遏蛋白RR_(TRES)是噬菌体434阻遏蛋白的衍生物,它是噬菌体434阻遏蛋白的N端DBD(1—69位氨基酸)组成的共价二聚体。
短句来源
     Using DNA Shuffling Method to Generate Thermosensitive CI Repressor Protein of λ Phage
     利用DNA改组技术产生λ噬菌体CI温敏阻遏蛋白
短句来源
更多       
  repressor proteins
     According to the characteristics of the interactions of λ cI repressor proteins with operators,the chemical kinetic model of their interactions is proposed.
     根据 λ噬菌体阻遏蛋白与操纵基因的相互作用特点 ,提出了他们相互作用的化学动力学模型 .
短句来源
  “阻遏蛋白”译为未确定词的双语例句
     Amino acid mutant site A43V locates in N-terminal headpiece of represser protein which binds specifically to DNAand amino acids mutant sites S93L, S102N, G315S, Q180R, M2491, I283T all locate in the core region of represser protein which binds to IPTG.
     氨基酸突变位点A43V位于阻遏蛋白氨基末端,是阻遏蛋白和操纵基因的结合区; S93L、S102N、G315S、Q180R、M249I、I283T均位于阻遏蛋白核心区,即阻遏蛋白和IPTG结合区。
短句来源
     To study binding funtion of 4 consensus bases in 16bp PUR box with purR protein, the directed site mutation for each was carried out which mutate from C to G, A to G, A to G, T to C, respectively.
     为研究16bP PUR box中除第3位的G与第14位的C外,余下6个完全保守碱基的4个在与PurR阻遏蛋白结合中的功能,对它们分别做了定点突变,使其分别从C、A、A和T突变为G、G、G和C。
短句来源
     Compared with the previous reported lad gene mutant site, A43V and S93L are new identified IPTG-inducible mutants.
     与已经报道lacI突变子的氨基酸突变位点比较表明:2W7(A43V)和3W1(S93L)是新发现的IPTG诱导型超级阻遏蛋白突变子;
短句来源
     coli transposon Tn10 was fused in frame with green fluorescent protein gene ( gfp ) from jellyfish Aequorea Victoria on an E.
     coli转座子Tn10的四环素阻遏蛋白基因 (tetR)共同构建到E .
短句来源
     DNA Shuffling and Screening for Obtaining Lactose Super-Repressor in Escherichia Coli
     DNA改组和筛选获得大肠杆菌乳糖操纵子超级阻遏蛋白
短句来源
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  repressor protein
The result strongly supported the idea that PUR box is the binding region of repressor protein and the 3rd position base G of PUR box is essential for the binding function with repressor protein.
      
This is the first time to extend target genes at which adaptive mutations could occur from structural genes involved in carbon metabolism and amino acid biosynthesis totrans regulatory gene coding repressor protein.
      
Common mechanisms for the regulation of B cell differentiation and transformation by the transcriptional repressor protein BCL-6
      
The BCL-6 transcriptional repressor protein is a critical regulator of normal B cell differentiation and BCL-6 has recently been shown to act as an oncogene in several mouse model systems.
      
Purification of Lac repressor protein using polymer displacement and immobilization of the protein
      
更多          
  aporepressor
It is shown that selectively deuteratedtrp aporepressor proteins display larger calculated NOESY intensities than those for the same interproton distances in the natural abundance protein.
      
Our results suggest that the Trp aporepressor can exist in the ground state in two distinct conformational states which differ in the microenvironment of the Trp residues.
      
Tre6P is the inducer, whereas Tre binds at the same binding site without reducing the aporepressor's affinity to the operator-DNA.
      
  repressor
Overexpression of ZNF569 in COS-7 cells dramatically inhibited the transcriptional repressor activities of SRE and AP-1, which was also confirmed by subcellular localization analysis.
      
The results suggested that ZNF569 protein might act as a transcriptional repressor in MARK signaling pathway to regulate cellular processes.
      
The effects of phytohormones involve not only induction of novel protein synthesis via activation of their gene expression, but also degradation of repressor proteins through the ubiquitin system.
      
The findings suggest a direct involvement of GATA-3 in regulation of the human IL-4 gene transcription as a repressor of the promoter activity.
      
A study was made of the antirestriction activity of Acidiphilium multivorum AIU 301 ArsR, a repressor of the ars operon which confers resistance to arsenite and arsenate and is contained in pKW301.
      
更多          
  repressible protein
Our results are consistent with the idea that the glucose-induced signaling pathway contains a glucose-repressible protein and that the protein is located before the point where intracellular acidification triggers activation of the pathway.
      
  其他


Chuangxtnmycin (CXM) and L-trp cause repression of trp-operon in wild strain of E. coli and indolepropanic acid (IPA) causes derepression of this strain. In CXM-resistant mutant strain, CXM and IPA both cause derepression,and interaction of CXM and IPA is competitive. However, L-trp results in neither repression nor derepression in the mutant, but it can counteract the activity of CXM or IPA. These results suggest that there are repression and derepression sites on repressor of E. coli. CXM can bind to both...

Chuangxtnmycin (CXM) and L-trp cause repression of trp-operon in wild strain of E. coli and indolepropanic acid (IPA) causes derepression of this strain. In CXM-resistant mutant strain, CXM and IPA both cause derepression,and interaction of CXM and IPA is competitive. However, L-trp results in neither repression nor derepression in the mutant, but it can counteract the activity of CXM or IPA. These results suggest that there are repression and derepression sites on repressor of E. coli. CXM can bind to both sites, but mainly to repression site. The structure of repression site of the CXM-resistant mutant is altered, so that CXM can not bind to the repression site and binds mainly to the derepression site, thus leading to the derepression of trp-operon.

测定创新霉素(CXM)及其类似物对大肠杆菌色氨酸操纵子表达的影响。结果发现,CXM和L-色氨酸(L-trp)对CXM敏感菌表现为阻遏作用,吲哚丙酸(IPA)则具有去阻遏作用;对CXM抗性变株,CXM与IPA均表现为去阻遏作用,L-trp不具阻遏作用,但可抵消CXM和IPA的去阻遏作用。提示阻遏蛋白和L-trp及其类以物至少有阻遏和去阻遏两个结合部位,CXM可以和这两个部位结合,在敏感菌中显示阻遏作用;在抗性变株中阻遏蛋白的阻遏部应结构改变,CXM显示去阻遏作用。

We have purified the a homogeneous trp aporepressor from an overproducing strain of E. coil carrying a plamid containing trpR positioned between the strongly regulated tac promoter and the rpoC transcription terminator. P11 cellulose chromatography is used in conjunction with a gel electrophoresis assay in which the repressor, when bound to the trp operator, protects an Rsa 1 restriction site from endonuclease cleavage. This method provides a simple way for studying the molecular basis of specific interaction...

We have purified the a homogeneous trp aporepressor from an overproducing strain of E. coil carrying a plamid containing trpR positioned between the strongly regulated tac promoter and the rpoC transcription terminator. P11 cellulose chromatography is used in conjunction with a gel electrophoresis assay in which the repressor, when bound to the trp operator, protects an Rsa 1 restriction site from endonuclease cleavage. This method provides a simple way for studying the molecular basis of specific interaction between proteins and DNA.

利用携带有克隆色氨酸阻遏蛋白基因的pJPR2质粒的大肠杆菌菌株,经过细菌培养、扩增及一系列生化方法,得到色氨酸阻遏蛋白的粗品,然后利用磷酸纤维素P11柱分离纯化得到单一的色氨酸阻遏蛋白,经SDS聚丙烯酰胺凝胶电泳鉴定和pRK9质粒中trpP/O片段保护实验证明,分离到的色氨酸阻遏蛋白是纯的,并具有生物活性。该方法和思维的建立,为研究核酸结合蛋白质的结构与功能提供了基础。

Purine represser protein, encoded by purR has shown to effect expression of purine structure genes except purB in de novo pathway in Salmonella typhimurium. However, up to date there is no direct evidence of the represser binding to operator DNA of these genes. Report here is the isolation and characterization of purine O6 mutants in Salmonella typhimurium. purD:: MudJ (lacZ Kanr) and purG:: MudJ(lacZ Kanr) were used as starting strains Both strains were mutagenezied by NTG, and derepressed mutants were selected...

Purine represser protein, encoded by purR has shown to effect expression of purine structure genes except purB in de novo pathway in Salmonella typhimurium. However, up to date there is no direct evidence of the represser binding to operator DNA of these genes. Report here is the isolation and characterization of purine O6 mutants in Salmonella typhimurium. purD:: MudJ (lacZ Kanr) and purG:: MudJ(lacZ Kanr) were used as starting strains Both strains were mutagenezied by NTG, and derepressed mutants were selected on the MacConkey plate containing an excess of adenosine (2mmol/L), 8 independent depressed mutants were obtained from purD:: MudJ (lacZ Kanr), 9 were obtained from purG:: MudJ (lacZ Kanr)- Transduction analysis and trans-acting or cis-acting test of the genes with merochromosome DNA tanden genetic duplication strains proved tha; 1 mutant for each group exert the constitute expression by cis-acting. This is first purine Oc mutant obtained from Salmonella typhimurium.

已有研究证明,编码阻遏蛋白的调节基因purR能调节嘌呤从头合成途径中除purB外所有结构基因的表达。但迄今还缺乏阻遏蛋白与这些基因的操纵基因相结合的直接证据。本文报道以嘌呤结构基因purD和purG的MudJ(lacZ,Kan~5)插入物为出发株,在外加过量腺嘌呤核苷(2mmol/L)的MacConkey平板上通过选择红色菌落分离O~c突变体的结果。从上述两株出发株分别获得了8株和9株独立的消阻遏突变体。共转导分析和顺反试验证明,两组突变体中各有1株顺式作用突变体(O~c)。这是在鼠伤寒沙门氏菌中首次获得的嘌呤O~c突变体,为研究阻遏蛋白与操纵基因相互作用提供了重要材料。

 
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